TRPC3 (NM_003305) Human Tagged ORF Clone

CAT#: RC210794

TRPC3 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant 2

Product Images

Circular map for RC210794

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  Western blot validation of overexpression lysate (Cat# LY401140) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210794 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TRPC3 protein (Cat# TP310794). The protein was produced from HEK293T cells transfected with TRPC3 cDNA clone (Cat# RC210794) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPC3
• Synonyms: SCA41; TRP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210794 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGAAGCCCATCCCTGAGACGCATGACAGTGATGCGGGAGAAGGGCCGGCGCCAGGCTGTCAGGG
  GCCCGGCCTTCATGTTCAATGACCGCGGCACCAGCCTCACCGCCGAGGAGGAGCGCTTCCTCGACGCCGC
  CGAGTACGGCAACATCCCAGTGGTGCGCAAGATGCTGGAGGAGTCCAAGACGCTGAACGTCAACTGCGTG
  GACTACATGGGCCAGAACGCGCTGCAGCTGGCTGTGGGCAACGAGCACCTGGAGGTGACCGAGCTGCTGC
  TCAAGAAGGAGAACCTGGCGCGCATTGGCGACGCCCTGCTGCTCGCCATCAGCAAGGGCTACGTGCGCAT
  CGTAGAGGCCATCCTCAACCACCCTGGCTTCGCGGCCAGCAAGCGTCTCACTCTGAGCCCCTGTGAGCAG
  GAGCTGCAGGACGACGACTTCTACGCTTACGACGAGGACGGCACGCGCTTCTCGCCGGACATCACCCCCA
  TCATCCTGGCGGCGCACTGCCAGAAATACGAAGTGGTGCACATGCTGCTGATGAAGGGTGCCAGGATCGA
  GCGGCCGCACGACTATTTCTGCAAGTGCGGGGACTGCATGGAGAAGCAGAGGCACGACTCCTTCAGCCAC
  TCACGCTCGAGGATCAATGCCTACAAGGGGCTGGCCAGCCCGGCTTACCTCTCATTGTCCAGCGAGGACC
  CGGTGCTTACGGCCCTAGAGCTCAGCAACGAGCTGGCCAAGCTGGCCAACATAGAGAAGGAGTTCAAGAA
  TGACTATCGGAAGCTCTCCATGCAATGCAAAGACTTTGTAGTGGGTGTGCTGGATCTCTGCCGAGACTCA
  GAAGAGGTAGAAGCCATTCTGAATGGAGATCTGGAATCAGCAGAGCCTCTGGAGGTACACAGGCACAAAG
  CTTCATTAAGTCGTGTCAAACTTGCCATTAAGTATGAAGTCAAAAAGTTTGTGGCTCATCCCAACTGCCA
  GCAGCAGCTCTTGACGATCTGGTATGAGAACCTCTCAGGCCTAAGGGAGCAGACCATAGCTATCAAGTGT
  CTCGTTGTGCTGGTCGTGGCCCTGGGCCTTCCATTCCTGGCCATTGGCTACTGGATCGCACCTTGCAGCA
  GGCTGGGGAAAATTCTGCGAAGCCCTTTTATGAAGTTTGTAGCACATGCAGCTTCTTTCATCATCTTCCT
  GGGTCTGCTTGTGTTCAATGCCTCAGACAGGTTCGAAGGCATCACCACGCTGCCCAATATCACAGTTACT
  GACTATCCCAAACAGATCTTCAGGGTGAAAACCACCCAGTTTACATGGACTGAAATGCTAATTATGGTCT
  GGGTTCTTGGAATGATGTGGTCTGAATGTAAAGAGCTCTGGCTGGAAGGACCTAGGGAATACATTTTGCA
  GTTGTGGAATGTGCTTGACTTTGGGATGCTGTCCATCTTCATTGCTGCTTTCACAGCCAGATTCCTAGCT
  TTCCTTCAGGCAACGAAGGCACAACAGTATGTGGACAGTTACGTCCAAGAGAGTGACCTCAGTGAAGTGA
  CACTCCCACCAGAGATACAGTATTTCACTTATGCTAGAGATAAATGGCTCCCTTCTGACCCTCAGATTAT
  ATCTGAAGGCCTTTATGCCATAGCTGTTGTGCTCAGCTTCTCTCGGATTGCGTACATCCTCCCTGCAAAT
  GAGAGCTTTGGCCCCCTGCAGATCTCTCTTGGAAGGACTGTAAAGGACATATTCAAGTTCATGGTCCTCT
  TTATTATGGTGTTTTTTGCCTTTATGATTGGCATGTTCATACTTTATTCTTACTACCTTGGGGCTAAAGT
  TAATGCTGCTTTTACCACTGTAGAAGAAAGTTTCAAGACTTTATTTTGGTCAATATTTGGGTTGTCTGAA
  GTGACTTCCGTTGTGCTCAAATATGATCACAAATTCATAGAAAATATTGGATACGTTCTTTATGGAATAT
  ACAATGTAACTATGGTGGTCGTTTTACTCAACATGCTAATTGCTATGATTAATAGCTCATATCAAGAAAT
  TGAGGATGACAGTGATGTAGAATGGAAGTTTGCTCGTTCAAAACTTTGGTTATCCTATTTTGATGATGGA
  AAAACATTACCTCCACCTTTCAGTCTAGTTCCTAGTCCAAAATCATTTGTTTATTTCATCATGCGAATTG
  TTAACTTTCCCAAATGCAGAAGGAGAAGGCTTCAGAAGGATATAGAAATGGGAATGGGTAACTCAAAGTC
  CAGGTTAAACCTCTTCACTCAGTCTAACTCAAGAGTTTTTGAATCACACAGTTTTAACAGCATTCTCAAT
  CAGCCAACACGTTATCAGCAGATAATGAAAAGACTTATAAAGCGGTATGTTTTGAAAGCACAAGTAGACA
  AAGAAAATGATGAAGTTAATGAAGGTGAATTAAAAGAAATCAAGCAAGATATCTCCAGCCTTCGTTATGA
  ACTTTTGGAAGACAAGAGCCAAGCAACTGAGGAATTAGCCATTCTAATTCATAAACTTAGTGAGAAACTG
  AATCCCAGCATGCTGAGATGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210794 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGSPSLRRMTVMREKGRRQAVRGPAFMFNDRGTSLTAEEERFLDAAEYGNIPVVRKMLEESKTLNVNCV
  DYMGQNALQLAVGNEHLEVTELLLKKENLARIGDALLLAISKGYVRIVEAILNHPGFAASKRLTLSPCEQ
  ELQDDDFYAYDEDGTRFSPDITPIILAAHCQKYEVVHMLLMKGARIERPHDYFCKCGDCMEKQRHDSFSH
  SRSRINAYKGLASPAYLSLSSEDPVLTALELSNELAKLANIEKEFKNDYRKLSMQCKDFVVGVLDLCRDS
  EEVEAILNGDLESAEPLEVHRHKASLSRVKLAIKYEVKKFVAHPNCQQQLLTIWYENLSGLREQTIAIKC
  LVVLVVALGLPFLAIGYWIAPCSRLGKILRSPFMKFVAHAASFIIFLGLLVFNASDRFEGITTLPNITVT
  DYPKQIFRVKTTQFTWTEMLIMVWVLGMMWSECKELWLEGPREYILQLWNVLDFGMLSIFIAAFTARFLA
  FLQATKAQQYVDSYVQESDLSEVTLPPEIQYFTYARDKWLPSDPQIISEGLYAIAVVLSFSRIAYILPAN
  ESFGPLQISLGRTVKDIFKFMVLFIMVFFAFMIGMFILYSYYLGAKVNAAFTTVEESFKTLFWSIFGLSE
  VTSVVLKYDHKFIENIGYVLYGIYNVTMVVVLLNMLIAMINSSYQEIEDDSDVEWKFARSKLWLSYFDDG
  KTLPPPFSLVPSPKSFVYFIMRIVNFPKCRRRRLQKDIEMGMGNSKSRLNLFTQSNSRVFESHSFNSILN
  QPTRYQQIMKRLIKRYVLKAQVDKENDEVNEGELKEIKQDISSLRYELLEDKSQATEELAILIHKLSEKL
  NPSMLRCE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003305
• ORF Size: 2544 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003305.2
• RefSeq Size: 3330 bp
• RefSeq ORF: 2547 bp
• Locus ID: 7222
• UniProt ID: Q13507
• Cytogenetics: 4q27
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 97.4 kDa
• Gene Summary: The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]