Coatomer subunit delta (ARCN1) (NM_001655) Human Tagged ORF Clone

CAT#: RC210778

ARCN1 (Myc-DDK-tagged)-Human archain 1 (ARCN1), transcript variant 1

Product Images

Circular map for RC210778

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ARCN1 (Cat# RC210778, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARCN1(Cat# TA504314). Positive lysates LY400624 (100ug) and LC400624 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400624) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210778 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ARCN1 protein (Cat# TP310778). The protein was produced from HEK293T cells transfected with ARCN1 cDNA clone (Cat# RC210778) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Coatomer subunit delta
• Synonyms: COPD; SRMMD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210778 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTGTTGGCAGCAGCGGTCTGCACAAAAGCAGGAAAGGCTATTGTTTCTCGACAGTTTGTGGAAA
  TGACCCGAACTCGGATTGAGGGCTTATTAGCAGCTTTTCCAAAGCTCATGAACACTGGAAAACAACATAC
  GTTTGTTGAAACAGAGAGTGTAAGATATGTCTACCAGCCTATGGAGAAACTGTATATGGTACTGATCACT
  ACCAAAAACAGCAACATTTTAGAAGATTTGGAGACCCTAAGGCTCTTCTCAAGAGTGATCCCTGAATATT
  GCCGAGCCTTAGAAGAGAATGAAATATCTGAGCACTGTTTTGATTTGATTTTTGCTTTTGATGAAATTGT
  CGCACTGGGATACCGGGAGAATGTTAACTTGGCACAGATCAGAACCTTCACAGAAATGGATTCTCATGAG
  GAGAAGGTGTTCAGAGCCGTCAGAGAGACTCAAGAACGTGAAGCTAAGGCTGAGATGCGTCGTAAAGCAA
  AGGAATTACAACAGGCCCGAAGAGATGCAGAGAGACAGGGCAAAAAAGCACCAGGATTTGGCGGATTTGG
  CAGCTCTGCAGTATCTGGAGGCAGCACAGCTGCCATGATCACAGAGACCATCATTGAAACTGATAAACCA
  AAAGTGGCACCTGCACCAGCCAGGCCTTCAGGCCCCAGCAAGGCTTTAAAACTTGGAGCCAAAGGAAAGG
  AAGTAGATAACTTTGTGGACAAATTAAAATCTGAAGGTGAAACCATCATGTCCTCTAGTATGGGCAAGCG
  TACTTCTGAAGCAACCAAAATGCATGCTCCACCCATTAATATGGAAAGTGTACATATGAAGATTGAAGAA
  AAGATAACATTAACCTGTGGACGAGACGGAGGATTACAGAATATGGAGTTGCATGGCATGATCATGCTTA
  GGATCTCAGATGACAAGTATGGCCGAATTCGTCTTCATGTGGAAAATGAAGATAAGAAAGGGGTGCAGCT
  ACAGACCCATCCAAATGTGGATAAAAAACTTTTCACTGCAGAGTCTCTAATTGGCCTGAAGAATCCAGAG
  AAGTCATTTCCAGTCAACAGTGACGTAGGGGTGCTAAAGTGGAGACTACAAACCACAGAGGAATCTTTTA
  TTCCACTGACAATTAATTGCTGGCCCTCGGAGAGTGGAAATGGCTGTGATGTCAACATAGAATATGAGCT
  ACAAGAAGATAATTTAGAACTGAATGATGTGGTTATCACCATCCCACTCCCGTCTGGTGTCGGCGCGCCT
  GTTATCGGTGAGATCGATGGGGAGTATCGACATGACAGTCGACGAAATACCCTGGAGTGGTGCCTGCCTG
  TGATTGATGCCAAAAATAAGAGTGGCAGCCTGGAGTTTAGCATTGCTGGGCAGCCCAATGACTTCTTCCC
  TGTTCAAGTTTCCTTTGTCTCCAAGAAAAATTACTGTAACATACAGGTTACCAAAGTGACCCAGGTAGAT
  GGAAACAGCCCCGTCAGGTTTTCCACAGAGACCACTTTCCTAGTGGATAAGTATGAAATTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210778 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLLAAAVCTKAGKAIVSRQFVEMTRTRIEGLLAAFPKLMNTGKQHTFVETESVRYVYQPMEKLYMVLIT
  TKNSNILEDLETLRLFSRVIPEYCRALEENEISEHCFDLIFAFDEIVALGYRENVNLAQIRTFTEMDSHE
  EKVFRAVRETQEREAKAEMRRKAKELQQARRDAERQGKKAPGFGGFGSSAVSGGSTAAMITETIIETDKP
  KVAPAPARPSGPSKALKLGAKGKEVDNFVDKLKSEGETIMSSSMGKRTSEATKMHAPPINMESVHMKIEE
  KITLTCGRDGGLQNMELHGMIMLRISDDKYGRIRLHVENEDKKGVQLQTHPNVDKKLFTAESLIGLKNPE
  KSFPVNSDVGVLKWRLQTTEESFIPLTINCWPSESGNGCDVNIEYELQEDNLELNDVVITIPLPSGVGAP
  VIGEIDGEYRHDSRRNTLEWCLPVIDAKNKSGSLEFSIAGQPNDFFPVQVSFVSKKNYCNIQVTKVTQVD
  GNSPVRFSTETTFLVDKYEIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001655
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001655.5
• RefSeq Size: 4032 bp
• RefSeq ORF: 1536 bp
• Locus ID: 372
• UniProt ID: P48444
• Cytogenetics: 11q23.3
• Domains: Adap_comp_sub
• MW: 57.2 kDa
• Gene Summary: This gene maps in a region, which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes, where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. [provided by RefSeq, Jul 2008]