DCK (NM_000788) Human Tagged ORF Clone

CAT#: RC210767

DCK (Myc-DDK-tagged)-Human deoxycytidine kinase (DCK)

Product Images

Circular map for RC210767

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DCK (Cat# RC210767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCK(Cat# TA502698). Positive lysates LY400272 (100ug) and LC400272 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400272) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210767 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DCK protein (Cat# TP310767). The protein was produced from HEK293T cells transfected with DCK cDNA clone (Cat# RC210767) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210767 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCCCGCCCAAGAGAAGCTGCCCGTCTTTCTCAGCCAGCTCTGAGGGGACCCGCATCAAGAAAA
  TCTCCATCGAAGGGAACATCGCTGCAGGGAAGTCAACATTTGTGAATATCCTTAAACAATTGTGTGAAGA
  TTGGGAAGTGGTTCCTGAACCTGTTGCCAGATGGTGCAATGTTCAAAGTACTCAAGATGAATTTGAGGAA
  CTTACAATGTCTCAGAAAAATGGTGGGAATGTTCTTCAGATGATGTATGAGAAACCTGAACGATGGTCTT
  TTACCTTCCAAACATATGCTTGTCTCAGTCGAATAAGAGCTCAGCTTGCCTCTCTGAATGGCAAGCTCAA
  AGATGCAGAGAAACCTGTATTATTTTTTGAACGATCTGTGTATAGTGACAGGTATATTTTTGCATCTAAT
  TTGTATGAATCTGAATGCATGAATGAGACAGAGTGGACAATTTATCAAGACTGGCATGACTGGATGAATA
  ACCAATTTGGCCAAAGCCTTGAATTGGATGGAATCATTTATCTTCAAGCCACTCCAGAGACATGCTTACA
  TAGAATATATTTACGGGGAAGAAATGAAGAGCAAGGCATTCCTCTTGAATATTTAGAGAAGCTTCATTAT
  AAACATGAAAGCTGGCTCCTGCATAGGACACTGAAAACCAACTTCGATTATCTTCAAGAGGTGCCTATCT
  TAACACTGGATGTTAATGAAGACTTTAAAGACAAATATGAAAGTCTGGTTGAAAAGGTCAAAGAGTTTTT
  GAGTACTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210767 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEE
  LTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASN
  LYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHY
  KHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000788
• ORF Size: 780 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000788.3
• RefSeq Size: 2618 bp
• RefSeq ORF: 783 bp
• Locus ID: 1633
• UniProt ID: P27707
• Cytogenetics: 4q13.3
• Domains: dNK
• Protein Families: Druggable Genome
• Protein Pathways: Purine metabolism, Pyrimidine metabolism
• MW: 30.5 kDa
• Gene Summary: Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]