DNA Ligase I (LIG1) (NM_000234) Human Tagged ORF Clone

CAT#: RC210760

LIG1 (Myc-DDK-tagged)-Human ligase I, DNA, ATP-dependent (LIG1)

Product Images

Circular map for RC210760

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  Western blot validation of overexpression lysate (Cat# LY424847) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210760 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LIG1 protein (Cat# TP310760). The protein was produced from HEK293T cells transfected with LIG1 cDNA clone (Cat# RC210760) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNA Ligase I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210760 representing NM_000234
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGAAGTATCATGTCATTTTTCCACCCCAAGAAAGAGGGTAAAGCAAAGAAGCCTGAGAAGGAGG
  CATCCAATAGCAGCAGAGAGACGGAGCCCCCTCCAAAGGCGGCACTGAAGGAGTGGAATGGAGTGGTGTC
  CGAGAGTGACTCTCCGGTGAAGAGGCCAGGGAGGAAGGCGGCCCGGGTCCTGGGCAGCGAAGGGGAAGAG
  GAGGATGAAGCCCTTAGCCCTGCTAAAGGCCAGAAGCCTGCCCTGGACTGCTCACAGGTCTCCCCGCCCC
  GTCCTGCCACATCTCCTGAGAACAATGCTTCCCTCTCTGACACCTCTCCCATGGACAGTTCCCCATCAGG
  GATTCCGAAGCGTCGCACAGCTCGGAAGCAGCTCCCGAAACGGACCATTCAGGAAGTCCTGGAAGAGCAG
  AGTGAGGACGAGGACAGAGAAGCCAAGAGGAAGAAGGAGGAGGAAGAAGAGGAGACCCCGAAAGAAAGCC
  TCACAGAGGCTGAAGTGGCCACAGAGAAGGAAGGAGAAGACGGGGACCAGCCCACCACGCCTCCCAAGCC
  CCTAAAGACCTCCAAAGCAGAGACCCCGACGGAAAGCGTTTCAGAGCCTGAGGTGGCCACGAAGCAGGAA
  CTGCAGGAGGAGGAAGAGCAGACCAAGCCTCCCCGCAGAGCTCCCAAGACGCTCAGCAGCTTCTTCACCC
  CCCGGAAGCCAGCAGTCAAAAAAGAAGTGAAGGAAGAGGAGCCAGGGGCTCCAGGAAAGGAGGGAGCTGC
  TGAGGGACCCCTGGATCCATCTGGTTACAATCCTGCCAAGAACAACTATCATCCCGTGGAAGATGCCTGC
  TGGAAACCGGGCCAGAAGGTTCCTTACCTGGCTGTGGCCCGGACGTTTGAGAAGATCGAGGAGGTGTCTG
  CTCGGCTCCGGATGGTGGAGACGCTGAGCAACTTGCTGCGCTCCGTGGTGGCCCTGTCGCCTCCAGACCT
  CCTCCCTGTCCTCTACCTCAGCCTCAACCACCTTGGGCCACCCCAGCAGGGCCTGGAGCTTGGCGTGGGT
  GATGGTGTCCTTCTCAAGGCAGTGGCCCAGGCCACAGGTCGGCAGCTGGAGTCCGTCCGGGCTGAGGCAG
  CCGAGAAAGGCGACGTGGGGCTGGTGGCCGAGAACAGCCGCAGCACCCAGAGGCTCATGCTGCCACCACC
  TCCGCTCACTGCCTCCGGGGTCTTCAGCAAGTTCCGCGACATCGCCAGGCTCACTGGCAGTGCTTCCACA
  GCCAAGAAGATAGACATCATCAAAGGCCTCTTTGTGGCCTGCCGCCACTCAGAAGCCCGGTTCATCGCTA
  GGTCCCTGAGCGGACGGCTGCGCCTTGGGCTGGCAGAGCAGTCGGTGCTGGCTGCCCTCTCCCAGGCAGT
  GAGCCTCACGCCCCCGGGCCAAGAATTCCCACCAGCCATGGTGGATGCTGGGAAGGGCAAGACAGCAGAG
  GCCAGAAAGACGTGGCTGGAGGAGCAAGGCATGATCCTGAAGCAGACGTTCTGCGAGGTTCCCGACCTGG
  ACCGAATTATCCCCGTGCTGCTGGAGCACGGCCTGGAACGTCTCCCGGAGCACTGCAAGCTGAGCCCAGG
  GATTCCCCTGAAACCAATGTTGGCCCATCCCACCCGGGGCATCAGCGAGGTCCTGAAACGCTTTGAGGAG
  GCAGCTTTCACCTGCGAATACAAATATGACGGGCAGAGGGCACAGATCCACGCCCTGGAAGGCGGGGAGG
  TGAAGATCTTCAGCAGGAATCAGGAAGACAACACTGGGAAGTACCCGGACATCATCAGCCGCATCCCCAA
  GATTAAACTCCCATCGGTCACATCCTTCATCCTGGACACCGAAGCCGTGGCTTGGGACCGGGAAAAGAAG
  CAGATCCAGCCATTCCAAGTGCTCACCACCCGCAAACGCAAGGAGGTGGATGCGTCTGAGATCCAGGTGC
  AGGTGTGTTTGTACGCCTTCGACCTCATCTACCTCAATGGAGAGTCCCTGGTACGTGAGCCCCTTTCCCG
  GCGCCGGCAGCTGCTCCGGGAGAACTTTGTGGAGACAGAGGGCGAGTTTGTCTTCGCCACCTCCCTGGAC
  ACCAAGGACATCGAGCAGATCGCCGAGTTCCTGGAGCAGTCAGTGAAAGACTCCTGCGAGGGGCTGATGG
  TGAAGACCCTGGATGTTGATGCCACCTACGAGATCGCCAAGAGATCGCACAACTGGCTCAAGCTGAAGAA
  GGACTACCTTGATGGCGTGGGTGACACCCTGGACCTGGTGGTGATCGGCGCCTACCTGGGCCGGGGGAAG
  CGGGCCGGCCGGTACGGGGGCTTCCTGCTGGCCTCCTACGACGAGGACAGTGAGGAGCTGCAGGCCATAT
  GCAAGCTTGGAACTGGCTTCAGTGATGAGGAGCTGGAGGAGCATCACCAGAGCCTCAAGGCGCTGGTGCT
  GCCCAGCCCACGCCCTTACGTGCGGATAGATGGCGCTGTGATTCCCGACCACTGGCTGGACCCCAGCGCT
  GTGTGGGAGGTGAAGTGCGCTGACCTCTCCCTCTCTCCCATCTACCCTGCTGCGCGGGGCCTGGTGGATA
  GTGACAAGGGCATCTCCCTTCGCTTCCCTCGGTTTATTCGAGTCCGTGAAGACAAGCAGCCGGAGCAGGC
  CACCACCAGTGCTCAGGTGGCCTGTTTGTACCGGAAGCAAAGTCAGATTCAGAACCAACAAGGCGAGGAC
  TCAGGCTCTGACCCTGAAGATACCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210760 representing NM_000234
  Red=Cloning site Green=Tags(s)
  
  MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESDSPVKRPGRKAARVLGSEGEE
  EDEALSPAKGQKPALDCSQVSPPRPATSPENNASLSDTSPMDSSPSGIPKRRTARKQLPKRTIQEVLEEQ
  SEDEDREAKRKKEEEEEETPKESLTEAEVATEKEGEDGDQPTTPPKPLKTSKAETPTESVSEPEVATKQE
  LQEEEEQTKPPRRAPKTLSSFFTPRKPAVKKEVKEEEPGAPGKEGAAEGPLDPSGYNPAKNNYHPVEDAC
  WKPGQKVPYLAVARTFEKIEEVSARLRMVETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLELGVG
  DGVLLKAVAQATGRQLESVRAEAAEKGDVGLVAENSRSTQRLMLPPPPLTASGVFSKFRDIARLTGSAST
  AKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAALSQAVSLTPPGQEFPPAMVDAGKGKTAE
  ARKTWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEE
  AAFTCEYKYDGQRAQIHALEGGEVKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFILDTEAVAWDREKK
  QIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLD
  TKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGK
  RAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSA
  VWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGED
  SGSDPEDTY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000234
• ORF Size: 2757 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000234.3
• RefSeq Size: 3083 bp
• RefSeq ORF: 2760 bp
• Locus ID: 3978
• UniProt ID: P18858
• Cytogenetics: 19q13.33
• Domains: DNA_ligase
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair, DNA replication, Mismatch repair, Nucleotide excision repair
• MW: 101.6 kDa
• Gene Summary: This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]