P5CS (ALDH18A1) (NM_002860) Human Tagged ORF Clone

CAT#: RC210726

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ALDH18A1 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 18 family, member A1 (ALDH18A1), nuclear gene encoding mitochondrial protein, transcript variant 1



  "NM_002860" in other vectors (5)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ALDH18A1
Synonyms ADCL3; ARCL3A; GSAS; P5CS; PYCS; SPG9; SPG9A; SPG9B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210726 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAGTCAAGTTTACCGCTGTGGGTTCCAGCCCTTCAACCAACATCTTCTGCCCTGGGTCAAGTGTA
CAACCGTCTTCAGATCTCATTGTATCCAGCCTTCAGTCATCAGACATGTTCGTTCTTGGAGCAACATCCC
GTTTATCACTGTACCCCTCAGTCGTACACATGGCAAGTCCTTCGCCCACCGCAGTGAGCTGAAGCATGCC
AAGAGAATCGTGGTGAAGCTCGGCAGTGCCGTGGTGACCCGAGGGGATGAATGTGGCCTGGCCCTGGGGC
GCTTGGCATCTATTGTTGAGCAGGTATCAGTGCTGCAGAATCAGGGCAGAGAGATGATGCTGGTGACCAG
TGGAGCCGTAGCCTTTGGCAAACAACGCTTGCGCCATGAGATCCTTCTGTCTCAGAGCGTGCGGCAGGCC
CTCCACTCGGGGCAGAACCAGCTGAAAGAAATGGCAATTCCAGTCTTAGAGGCACGAGCCTGTGCAGCTG
CCGGACAGAGTGGGCTGATGGCCTTGTATGAGGCTATGTTTACCCAGTACAGCATCTGTGCTGCCCAGAT
TTTGGTGACCAATTTGGATTTCCATGATGAGCAGAAGCGCCGGAACCTCAATGGAACACTTCATGAACTC
CTTAGAATGAACATTGTCCCCATTGTCAACACAAATGATGCTGTTGTCCCCCCAGCTGAGCCCAACAGTG
ACCTGCAGGGGGTAAATGTTATTAGTGTTAAAGATAATGATAGCCTGGCTGCCCGACTGGCTGTGGAAAT
GAAAACTGATCTCTTGATTGTTCTTTCAGATGTAGAAGGCCTTTTTGACAGCCCCCCAGGTTCAGATGAT
GCAAAGCTTATTGATATATTTTATCCCGGAGATCAGCAGTCTGTGACATTTGGAATCAAGTCTAGAGTGG
GAATGGGTGGCATGGAAGCCAAGGTGAAAGCAGCCCTCTGGGCTTTGCAAGGTGGCACTTCTGTTGTTAT
TGCCAATGGAACCCACCCAAAGGTGTCTGGGCACGTCATCACAGACATTGTGGAGGGGAAGAAAGTTGGT
ACCTTCTTTTCAGAAGTAAAGCCTGCAGGCCCTACTGTTGAGCAGCAGGGAGAAATGGCGCGATCTGGAG
GAAGGATGTTGGCCACCTTGGAACCTGAGCAGAGAGCAGAAATTATCCATCATCTGGCTGATCTGTTGAC
GGACCAGCGTGATGAGATCCTGTTAGCCAACAAAAAAGACTTGGAGGAGGCAGAGGGGAGACTTGCAGCT
CCTCTGCTGAAACGTTTAAGCCTCTCCACATCCAAATTGAACAGCCTGGCCATCGGTCTGCGACAGATCG
CAGCCTCCTCCCAGGACAGCGTGGGACGTGTTTTGCGCCGCACCCGAATCGCCAAAAACTTGGAACTGGA
ACAAGTGACTGTCCCAATTGGAGTTCTGCTGGTGATCTTTGAATCTCGTCCTGACTGTCTACCCCAGGTG
GCAGCTTTGGCTATCGCAAGTGGCAATGGCTTGTTACTCAAAGGAGGGAAGGAGGCTGCACACAGCAACC
GGATTCTCCACCTCCTGACCCAGGAGGCTCTCTCAATCCATGGAGTCAAGGAGGCCGTGCAACTGGTGAA
TACCAGAGAAGAAGTTGAAGATCTTTGCCGCCTAGACAAAATGATAGATCTGATCATTCCACGTGGCTCT
TCCCAGCTGGTCAGAGACATCCAGAAAGCTGCTAAGGGGATTCCAGTGATGGGGCACAGCGAAGGGATCT
GTCACATGTATGTGGATTCCGAGGCCAGTGTTGATAAGGTCACCAGGCTAGTCAGAGACTCTAAATGTGA
ATATCCAGCTGCCTGTAATGCTTTGGAGACTTTGTTAATCCACCGGGATCTGCTCAGGACACCATTATTT
GACCAGATCATTGATATGCTGAGAGTGGAACAGGTAAAAATTCATGCAGGCCCCAAATTTGCCTCCTATC
TGACCTTCAGCCCCTCCGAAGTGAAGTCACTCCGAACTGAGTATGGGGACCTGGAATTATGCATTGAAGT
AGTGGACAACGTTCAGGATGCCATTGACCACATCCACAAGTATGGCAGCTCCCACACGGATGTCATCGTC
ACAGAGGACGAAAACACAGCGGAGTTCTTCCTGCAGCACGTAGACAGTGCCTGTGTGTTCTGGAATGCCA
GCACTCGCTTTTCTGATGGTTACCGCTTTGGACTGGGAGCTGAAGTGGGAATCAGTACATCGAGAATCCA
CGCCCGGGGACCAGTAGGACTTGAGGGACTGCTTACTACTAAGTGGCTGCTGCGAGGGAAGGACCACGTG
GTCTCAGATTTCTCAGAGCATGGAAGTTTAAAATATCTTCATGAGAACCTCCCTATTCCTCAGAGAAACA
CCAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210726 protein sequence
Red=Cloning site Green=Tags(s)

MLSQVYRCGFQPFNQHLLPWVKCTTVFRSHCIQPSVIRHVRSWSNIPFITVPLSRTHGKSFAHRSELKHA
KRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQA
LHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHEL
LRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDD
AKLIDIFYPGDQQSVTFGIKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVG
TFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIIHHLADLLTDQRDEILLANKKDLEEAEGRLAA
PLLKRLSLSTSKLNSLAIGLRQIAASSQDSVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQV
AALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVKEAVQLVNTREEVEDLCRLDKMIDLIIPRGS
SQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLF
DQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKSLRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIV
TEDENTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGKDHV
VSDFSEHGSLKYLHENLPIPQRNTN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002860
ORF Size 2385 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002860.4
RefSeq Size 3470 bp
RefSeq ORF 2388 bp
Locus ID 5832
UniProt ID P54886
Cytogenetics 10q24.1
Domains aakinase, aldedh
Protein Families Druggable Genome
Protein Pathways Arginine and proline metabolism, Metabolic pathways
MW 87.3 kDa
Gene Summary This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene. [provided by RefSeq, Jul 2008]

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