RAB5C (NM_201434) Human Tagged ORF Clone

CAT#: RC210698

RAB5C (Myc-DDK-tagged)-Human RAB5C, member RAS oncogene family (RAB5C), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY404416) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210698 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RAB5C protein (Cat# TP310698). The protein was produced from HEK293T cells transfected with RAB5C cDNA clone (Cat# RC210698) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAB5C
• Synonyms: L1880; RAB5CL; RAB5L; RABL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210698 representing NM_201434
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGTCGGGGAGGCGCAGCACGACCCAATGGACCAGCTGCTGGGAACAAGATCTGTCAATTTAAGC
  TGGTTCTGCTGGGGGAGTCTGCGGTAGGCAAATCCAGCCTCGTCCTCCGCTTTGTCAAGGGACAGTTTCA
  CGAGTACCAGGAGAGCACAATTGGAGCGGCCTTCCTCACACAGACTGTCTGCCTGGATGACACAACAGTC
  AAGTTTGAGATCTGGGACACAGCTGGACAGGAGCGGTATCACAGCCTGGCCCCCATGTACTATCGGGGGG
  CCCAGGCTGCCATCGTGGTCTATGACATCACCAACACAGATACATTTGCACGGGCCAAGAACTGGGTGAA
  GGAGCTACAGAGGCAGGCCAGCCCCAACATCGTCATTGCACTCGCGGGTAACAAGGCAGACCTGGCCAGC
  AAGAGAGCCGTGGAATTCCAGGAAGCACAAGCCTATGCAGACGACAACAGTTTGCTGTTCATGGAGACAT
  CAGCAAAGACTGCAATGAACGTGAACGAAATCTTCATGGCAATAGCTAAGAAGCTTCCCAAGAACGAGCC
  CCAGAATGCAACTGGTGCTCCAGGCCGAAACCGAGGTGTGGACCTCCAGGAGAACAACCCAGCCAGCCGG
  AGCCAGTGCTGCAGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210698 representing NM_201434
  Red=Cloning site Green=Tags(s)
  
  MAGRGGAARPNGPAAGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDTTV
  KFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNTDTFARAKNWVKELQRQASPNIVIALAGNKADLAS
  KRAVEFQEAQAYADDNSLLFMETSAKTAMNVNEIFMAIAKKLPKNEPQNATGAPGRNRGVDLQENNPASR
  SQCCSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201434
• ORF Size: 648 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201434.3
• RefSeq Size: 1791 bp
• RefSeq ORF: 651 bp
• Locus ID: 5878
• UniProt ID: P51148
• Cytogenetics: 17q21.2
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• MW: 23.3 kDa
• Gene Summary: Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).[supplied by OMIM, Nov 2010]