HSDL2 (NM_032303) Human Tagged ORF Clone

CAT#: RC210663

HSDL2 (Myc-DDK-tagged)-Human hydroxysteroid dehydrogenase like 2 (HSDL2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410245) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210663 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HSDL2 protein (Cat# TP310663). The protein was produced from HEK293T cells transfected with HSDL2 cDNA clone (Cat# RC210663) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HSDL2
• Synonyms: C9orf99; SDR13C1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210663 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTACCCAACACCGGGAGGCTGGCAGGATGTACAGTTTTTATCACAGGTGCAAGCCGTGGCATTGGCA
  AAGCTATTGCATTGAAAGCAGCAAAGGATGGAGCAAATATTGTTATTGCTGCAAAGACCGCCCAGCCACA
  TCCAAAACTTCTAGGCACAATCTATACTGCTGCTGAAGAAATTGAAGCAGTTGGAGGAAAGGCCTTGCCA
  TGTATTGTTGATGTGAGAGATGAACAGCAGATCAGTGCTGCAGTGGAGAAAGCCATCAAGAAATTTGGAG
  GAATTGATATTCTGGTAAATAATGCCAGTGCCATTAGTTTGACCAATACATTGGACACACCTACCAAGAG
  ATTGGATCTGATGATGAACGTGAACACCAGAGGCACCTACCTTGCATCTAAAGCATGTATTCCTTATTTG
  AAAAAGAGCAAAGTTGCTCATATCCTCAATATCAGTCCACCACTGAACCTAAATCCAGTTTGGTTCAAAC
  AGCACTGTGCTTATACCATTGCTAAGTATGGTATGTCTATGTATGTGCTTGGAATGGCAGAAGAATTTAA
  AGGTGAAATTGCAGTCAATGCATTATGGCCTAAAACAGCCATACACACTGCTGCTATGGATATGCTGGGA
  GGACCTGGTATCGAAAGCCAGTGTAGAAAAGTTGATATCATTGCAGATGCAGCATATTCCATTTTCCAAA
  AGCCAAAAAGTTTTACTGGCAACTTTGTCATTGATGAAAATATCTTAAAAGAAGAAGGAATAGAAAATTT
  TGACGTTTATGCAATTAAACCAGGTCATCCTTTGCAACCAGATTTCTTCTTAGATGAATACCCAGAAGCA
  GTTAGCAAGAAAGTGGAATCAACTGGTGCTGTTCCAGAATTCAAAGAAGAGAAACTGCAGCTGCAACCAA
  AACCACGTTCTGGAGCTGTGGAAGAAACATTTAGAATTGTTAAGGACTCTCTCAGTGATGATGTTGTTAA
  AGCCACTCAAGCAATCTATCTGTTTGAACTCTCCGGTGAAGATGGTGGCACGTGGTTTCTTGATCTGAAA
  AGCAAGGGTGGGAATGTCGGATATGGAGAGCCTTCTGATCAGGCAGATGTGGTGATGAGTATGACTACTG
  ATGACTTTGTAAAAATGTTTTCAGGGAAACTAAAACCAACAATGGCATTCATGTCAGGGAAATTGAAGAT
  TAAAGGTAACATGGCCCTAGCAATCAAATTGGAGAAGCTAATGAATCAGATGAATGCCAGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210663 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPNTGRLAGCTVFITGASRGIGKAIALKAAKDGANIVIAAKTAQPHPKLLGTIYTAAEEIEAVGGKALP
  CIVDVRDEQQISAAVEKAIKKFGGIDILVNNASAISLTNTLDTPTKRLDLMMNVNTRGTYLASKACIPYL
  KKSKVAHILNISPPLNLNPVWFKQHCAYTIAKYGMSMYVLGMAEEFKGEIAVNALWPKTAIHTAAMDMLG
  GPGIESQCRKVDIIADAAYSIFQKPKSFTGNFVIDENILKEEGIENFDVYAIKPGHPLQPDFFLDEYPEA
  VSKKVESTGAVPEFKEEKLQLQPKPRSGAVEETFRIVKDSLSDDVVKATQAIYLFELSGEDGGTWFLDLK
  SKGGNVGYGEPSDQADVVMSMTTDDFVKMFSGKLKPTMAFMSGKLKIKGNMALAIKLEKLMNQMNARL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032303
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032303.5
• RefSeq Size: 3393 bp
• RefSeq ORF: 1257 bp
• Locus ID: 84263
• UniProt ID: Q6YN16
• Cytogenetics: 9q32
• Domains: SCP2
• Protein Families: Druggable Genome
• MW: 45.4 kDa
• Gene Summary: Has apparently no steroid dehydrogenase activity.[UniProtKB/Swiss-Prot Function]