HSDL2 (NM_032303) Human Tagged ORF Clone

CAT#: RC210663

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HSDL2 (Myc-DDK-tagged)-Human hydroxysteroid dehydrogenase like 2 (HSDL2), transcript variant 1



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol HSDL2
Synonyms C9orf99; SDR13C1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210663 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTACCCAACACCGGGAGGCTGGCAGGATGTACAGTTTTTATCACAGGTGCAAGCCGTGGCATTGGCA
AAGCTATTGCATTGAAAGCAGCAAAGGATGGAGCAAATATTGTTATTGCTGCAAAGACCGCCCAGCCACA
TCCAAAACTTCTAGGCACAATCTATACTGCTGCTGAAGAAATTGAAGCAGTTGGAGGAAAGGCCTTGCCA
TGTATTGTTGATGTGAGAGATGAACAGCAGATCAGTGCTGCAGTGGAGAAAGCCATCAAGAAATTTGGAG
GAATTGATATTCTGGTAAATAATGCCAGTGCCATTAGTTTGACCAATACATTGGACACACCTACCAAGAG
ATTGGATCTGATGATGAACGTGAACACCAGAGGCACCTACCTTGCATCTAAAGCATGTATTCCTTATTTG
AAAAAGAGCAAAGTTGCTCATATCCTCAATATCAGTCCACCACTGAACCTAAATCCAGTTTGGTTCAAAC
AGCACTGTGCTTATACCATTGCTAAGTATGGTATGTCTATGTATGTGCTTGGAATGGCAGAAGAATTTAA
AGGTGAAATTGCAGTCAATGCATTATGGCCTAAAACAGCCATACACACTGCTGCTATGGATATGCTGGGA
GGACCTGGTATCGAAAGCCAGTGTAGAAAAGTTGATATCATTGCAGATGCAGCATATTCCATTTTCCAAA
AGCCAAAAAGTTTTACTGGCAACTTTGTCATTGATGAAAATATCTTAAAAGAAGAAGGAATAGAAAATTT
TGACGTTTATGCAATTAAACCAGGTCATCCTTTGCAACCAGATTTCTTCTTAGATGAATACCCAGAAGCA
GTTAGCAAGAAAGTGGAATCAACTGGTGCTGTTCCAGAATTCAAAGAAGAGAAACTGCAGCTGCAACCAA
AACCACGTTCTGGAGCTGTGGAAGAAACATTTAGAATTGTTAAGGACTCTCTCAGTGATGATGTTGTTAA
AGCCACTCAAGCAATCTATCTGTTTGAACTCTCCGGTGAAGATGGTGGCACGTGGTTTCTTGATCTGAAA
AGCAAGGGTGGGAATGTCGGATATGGAGAGCCTTCTGATCAGGCAGATGTGGTGATGAGTATGACTACTG
ATGACTTTGTAAAAATGTTTTCAGGGAAACTAAAACCAACAATGGCATTCATGTCAGGGAAATTGAAGAT
TAAAGGTAACATGGCCCTAGCAATCAAATTGGAGAAGCTAATGAATCAGATGAATGCCAGACTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210663 protein sequence
Red=Cloning site Green=Tags(s)

MLPNTGRLAGCTVFITGASRGIGKAIALKAAKDGANIVIAAKTAQPHPKLLGTIYTAAEEIEAVGGKALP
CIVDVRDEQQISAAVEKAIKKFGGIDILVNNASAISLTNTLDTPTKRLDLMMNVNTRGTYLASKACIPYL
KKSKVAHILNISPPLNLNPVWFKQHCAYTIAKYGMSMYVLGMAEEFKGEIAVNALWPKTAIHTAAMDMLG
GPGIESQCRKVDIIADAAYSIFQKPKSFTGNFVIDENILKEEGIENFDVYAIKPGHPLQPDFFLDEYPEA
VSKKVESTGAVPEFKEEKLQLQPKPRSGAVEETFRIVKDSLSDDVVKATQAIYLFELSGEDGGTWFLDLK
SKGGNVGYGEPSDQADVVMSMTTDDFVKMFSGKLKPTMAFMSGKLKIKGNMALAIKLEKLMNQMNARL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032303
ORF Size 1254 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032303.5
RefSeq Size 3393 bp
RefSeq ORF 1257 bp
Locus ID 84263
UniProt ID Q6YN16
Cytogenetics 9q32
Domains SCP2
Protein Families Druggable Genome
MW 45.4 kDa
Gene Summary Has apparently no steroid dehydrogenase activity.[UniProtKB/Swiss-Prot Function]
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