RPS15 (NM_001018) Human Tagged ORF Clone
RPS15 (Myc-DDK-tagged)-Human ribosomal protein S15 (RPS15)
|Product Name||RPS15 (NM_001018) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC210640 representing NM_001018
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210640 representing NM_001018
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||435 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001018.1, NM_001018.2, NM_001018.3, NM_001018.4, NP_001009.1|
|Gene Summary||Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]|
|The use of this cDNA Clones has been cited in the following citations:|
Genetic evolution in chronic lymphocytic leukaemia
,Delgado, J;Villamor, N;López-Guillermo, A;Campo, E;,
Best Pract Res Clin Haematol 2016
,PubMed ID 27742073
Whole-exome sequencing in relapsing chronic lymphocytic leukemia: clinical impact of recurrent RPS15 mutations
,Ljungström, V;Cortese, D;Young, E;Pandzic, T;Mansouri, L;Plevova, K;Ntoufa, S;Baliakas, P;Clifford, R;Sutton, LA;Blakemore, S;Stavroyianni, N;Agathangelidis, A;Rossi, D;Höglund, M;Kotaskova, J;Juliusson, G;Belessi, C;Chiorazzi, N;Panagiotidis, P;Langerak, AW;Smedby, KE;Oscier, D;Gaidano, G;Schuh, A;Davi, F;Pott, C;Strefford, JC;Trentin, L;Pospisilova, S;Ghia, P;Stamatopoulos, K;Sjöblom, T;Rosenquist, R;,
Blood Dec 2015
,PubMed ID 26675346
|cDNA Clone Resources|
|SC119525||RPS15 (untagged)-Human ribosomal protein S15 (RPS15)||
|SC324559||RPS15 (untagged)-Human ribosomal protein S15 (RPS15)||
|RG210640||RPS15 (GFP-tagged) - Human ribosomal protein S15 (RPS15)||
|RC210640L3||Lenti ORF clone of Human ribosomal protein S15 (RPS15), Myc-DDK-tagged||
|RC210640L4||Lenti ORF clone of Human ribosomal protein S15 (RPS15), mGFP tagged||