MORC3 (NM_015358) Human Tagged ORF Clone

CAT#: RC210530

MORC3 (Myc-DDK-tagged)-Human MORC family CW-type zinc finger 3 (MORC3)

Product Images

Circular map for RC210530

• 

  Western blot validation of overexpression lysate (Cat# LY414617) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210530 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MORC3 protein (Cat# TP310530). The protein was produced from HEK293T cells transfected with MORC3 cDNA clone (Cat# RC210530) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MORC3
• Synonyms: NXP2; ZCW5; ZCWCC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210530 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCAGCCACCCCGCGGGATACGCCTCAGCGCGCTTTGCCCGAAGTTTTTACATACAAATTCTA
  CTAGTCACACCTGGCCATTCAGTGCAGTTGCTGAATTAATAGATAATGCTTATGATCCTGATGTGAACGC
  TAAACAAATATGGATTGACAAAACAGTGATAAATGACCATATATGCTTGACATTCACCGACAATGGGAAT
  GGTATGACTTCTGATAAATTACATAAAATGCTAAGCTTTGGCTTCAGTGACAAAGTCACCATGAATGGTC
  ATGTCCCAGTTGGATTATATGGGAATGGCTTCAAGTCGGGTTCTATGCGTCTGGGTAAAGACGCAATCGT
  TTTTACCAAAAATGGAGAAAGCATGAGCGTGGGCCTTTTGTCTCAGACCTACTTGGAAGTCATAAAAGCG
  GAGCATGTTGTTGTTCCAATAGTGGCATTCAACAAGCACCGACAGATGATTAATTTAGCAGAATCAAAAG
  CCAGCCTTGCTGCAATTCTGGAACATTCTCTGTTTTCCACGGAACAGAAGTTACTGGCAGAACTTGATGC
  TATTATAGGCAAGAAGGGGACGAGGATCATCATTTGGAATCTTAGAAGCTACAAAAATGCAACAGAGTTC
  GATTTTGAAAAGGATAAATATGATATCAGAATTCCCGAGGATTTAGATGAGATAACAGGGAAGAAGGGGT
  ACAAGAAGCAGGAAAGGATGGACCAGATTGCCCCTGAGAGTGACTATTCCCTGAGGGCTTATTGCAGTAT
  ATTATATCTAAAGCCAAGAATGCAGATCATCCTACGTGGACAGAAAGTGAAGACACAGCTGGTTTCGAAG
  AGTCTTGCCTACATCGAACGTGATGTTTATCGACCAAAATTTTTATCTAAAACAGTGAGAATTACCTTTG
  GATTCAACTGCAGAAATAAAGATCATTATGGGATAATGATGTATCACAGAAATAGACTCATCAAAGCTTA
  TGAAAAAGTTGGATGTCAGTTAAGGGCAAACAACATGGGTGTTGGAGTGGTTGGAATTATAGAGTGTAAT
  TTCCTTAAGCCAACTCATAATAAACAAGATTTCGACTATACTAATGAGTACAGACTTACAATAACAGCAC
  TAGGAGAAAAGCTGAATGATTACTGGAATGAAATGAAAGTGAAGAAAAATACAGAATATCCTCTAAATTT
  GCCAGTTGAAGATATACAGAAGCGTCCTGATCAGACATGGGTTCAGTGTGATGCCTGTCTAAAGTGGCGG
  AAATTACCTGATGGGATGGATCAACTTCCTGAAAAATGGTATTGCTCCAATAACCCTGACCCACAGTTCA
  GAAATTGTGAGGTTCCAGAAGAACCTGAAGATGAGGATTTGGTACATCCCACTTATGAAAAAACCTACAA
  AAAGACCAACAAGGAAAAATTCAGGATCAGACAACCGGAAATGATCCCTCGGATTAATGCTGAACTGTTG
  TTTCGGCCAACTGCTCTTTCAACTCCAAGCTTTTCTTCTCCTAAGGAAAGTGTTCCAAGAAGACATCTTT
  CAGAAGGAACAAATTCTTATGCGACAAGACTTCTAAATAATCATCAAGTTCCACCTCAGTCTGAACCTGA
  GAGCAACAGCTTGAAACGGAGACTTTCTACTCGTTCCTCAATTTTGAATGCAAAGAATCGGAGATTGAGT
  AGTCAGTTTGAAAATTCAGTTTATAAAGGTGATGATGATGATGGAGATGTCATCATCTTAGAAGAAAACA
  GTACCCCCAAACCTGCAGTAGATCATGATATTGACATGAAATCAGAACAGAGTCACGTTGAGCAAGGTGG
  TGTTCAGGTTGAGTTTGTGGGTGACAGTGAACCTTGTGGCCAAACTGGTTCAACAAGCACCTCATCATCC
  CGATGCGACCAGGGAAATACTGCAGCTACCCAGACTGAAGTACCAAGTTTAGTTGTTAAAAAAGAAGAAA
  CTGTTGAAGACGAGATAGACGTAAGAAATGATGCAGTGATTCTGCCCTCCTGTGTAGAAGCTGAAGCAAA
  GATACATGAAACCCAGGAAACCACCGATAAATCTGCAGATGATGCAGGCTGCCAATTACAAGAACTGAGA
  AACCAGCTACTCCTTGTCACTGAGGAAAAAGAGAATTATAAAAGACAGTGTCATATGTTTACTGATCAAA
  TCAAAGTGTTACAACAGAGGATACTAGAAATGAATGACAAGTATGTTAAGAAAGAAACTTGCCATCAGTC
  CACTGAAACCGATGCTGTATTTTTACTTGAAAGTATTAATGGCAAATCTGAAAGTCCAGACCATATGGTA
  TCTCAGTATCAGCAAGCTTTGGAAGAAATAGAAAGGCTGAAAAAACAATGTAGTGCTTTGCAACATGTAA
  AGGCTGAATGCAGCCAGTGTTCCAATAATGAGAGTAAAAGTGAAATGGATGAGATGGCTGTGCAGCTTGA
  CGATGTGTTTAGACAACTGGACAAATGCAGTATTGAGAGGGACCAGTATAAAAGTGAGGTTGAATTGCTG
  GAAATGGAAAAGTCACAAATCCGTTCACAGTGTGAAGAACTCAAAACTGAAGTAGAACAGTTAAAATCTA
  CAAATCAACAGACGGCAACAGATGTTTCAACATCAAGTAACATTGAGGAGTCTGTAAATCATATGGATGG
  AGAAAGCCTCAAACTCCGATCTCTTCGAGTTAACGTAGGACAACTGCTGGCTATGATTGTGCCTGATCTT
  GATCTTCAGCAAGTGAATTACGATGTTGATGTAGTTGATGAGATTTTAGGACAAGTTGTTGAACAAATGA
  GTGAAATCAGTAGTACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210530 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAQPPRGIRLSALCPKFLHTNSTSHTWPFSAVAELIDNAYDPDVNAKQIWIDKTVINDHICLTFTDNGN
  GMTSDKLHKMLSFGFSDKVTMNGHVPVGLYGNGFKSGSMRLGKDAIVFTKNGESMSVGLLSQTYLEVIKA
  EHVVVPIVAFNKHRQMINLAESKASLAAILEHSLFSTEQKLLAELDAIIGKKGTRIIIWNLRSYKNATEF
  DFEKDKYDIRIPEDLDEITGKKGYKKQERMDQIAPESDYSLRAYCSILYLKPRMQIILRGQKVKTQLVSK
  SLAYIERDVYRPKFLSKTVRITFGFNCRNKDHYGIMMYHRNRLIKAYEKVGCQLRANNMGVGVVGIIECN
  FLKPTHNKQDFDYTNEYRLTITALGEKLNDYWNEMKVKKNTEYPLNLPVEDIQKRPDQTWVQCDACLKWR
  KLPDGMDQLPEKWYCSNNPDPQFRNCEVPEEPEDEDLVHPTYEKTYKKTNKEKFRIRQPEMIPRINAELL
  FRPTALSTPSFSSPKESVPRRHLSEGTNSYATRLLNNHQVPPQSEPESNSLKRRLSTRSSILNAKNRRLS
  SQFENSVYKGDDDDGDVIILEENSTPKPAVDHDIDMKSEQSHVEQGGVQVEFVGDSEPCGQTGSTSTSSS
  RCDQGNTAATQTEVPSLVVKKEETVEDEIDVRNDAVILPSCVEAEAKIHETQETTDKSADDAGCQLQELR
  NQLLLVTEEKENYKRQCHMFTDQIKVLQQRILEMNDKYVKKETCHQSTETDAVFLLESINGKSESPDHMV
  SQYQQALEEIERLKKQCSALQHVKAECSQCSNNESKSEMDEMAVQLDDVFRQLDKCSIERDQYKSEVELL
  EMEKSQIRSQCEELKTEVEQLKSTNQQTATDVSTSSNIEESVNHMDGESLKLRSLRVNVGQLLAMIVPDL
  DLQQVNYDVDVVDEILGQVVEQMSEISST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015358
• ORF Size: 2817 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015358.3
• RefSeq Size: 4246 bp
• RefSeq ORF: 2820 bp
• Locus ID: 23515
• UniProt ID: Q14149
• Cytogenetics: 21q22.12
• MW: 107 kDa
• Gene Summary: This gene encodes a protein that localizes to the nuclear matrix and forms nuclear bodies via an ATP-dependent mechanism. The protein is predicted to have coiled-coil and zinc finger domains and has RNA binding activity. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2016]