UNC93B (UNC93B1) (NM_030930) Human Tagged ORF Clone

CAT#: RC210505

UNC93B1 (Myc-DDK-tagged)-Human unc-93 homolog B1 (C. elegans) (UNC93B1)

Product Images

Circular map for RC210505

• 

  Western blot validation of overexpression lysate (Cat# LY410646) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210505 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UNC93B
• Synonyms: IIAE1; Unc-93B1; UNC93; UNC93B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210505 representing NM_030930
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGGAGCCGCCGCTCTACCCGATGGCGGGGGCTGCGGGGCCGCAGGGCGACGAGGACCTGCTCG
  GGGTCCCGGACGGGCCCGAGGCCCCGCTGGACGAGCTGGTGGGCGCGTACCCCAACTACAACGAGGAGGA
  GGAGGAGCGCCGCTACTACCGCCGCAAGCGCCTGGGCGTGCTCAAGAACGTGCTGGCTGCCAGCGCCGGG
  GGCATGCTCACCTACGGCGTCTACCTGGGCCTCCTGCAGATGCAGCTGATCCTGCACTACGACGAGACCT
  ACCGCGAGGTGAAGTATGGCAACATGGGGCTGCCCGACATCGACAGCAAAATGCTGATGGGCATCAACGT
  GACTCCCATCGCCGCCCTGCTCTACACACCTGTGCTCATCAGGTTTTTTGGAACGAAGTGGATGATGTTC
  CTCGCTGTGGGCATCTACGCCCTCTTTGTCTCCACCAACTACTGGGAGCGCTACTACACGCTTGTGCCCT
  CGGCTGTGGCCCTGGGCATGGCCATCGTGCCTCTTTGGGCTTCCATGGGCAACTACATCACCAGGATGGC
  GCAGAAGTACCATGAGTACTCCCACTACAAGGAGCAGGATGGGCAGGGGATGAAGCAGCGGCCTCCGCGG
  GGCTCCCACGCGCCCTATCTCCTGGTCTTCCAAGCCATCTTCTACAGCTTCTTCCATCTGAGCTTCGCCT
  GCGCCCAGCTGCCCATGATTTATTTCCTGAACCACTACCTGTATGACCTGAACCACACGCTGTACAATGT
  GCAGAGCTGCGGCACCAACAGCCACGGGATCCTCAGCGGCTTCAACAAGACGGTTCTGCGGACGCTCCCG
  CGGAGCGGAAACCTCATTGTGGTGGAGAGCGTGCTCATGGCAGTGGCCTTCCTGGCCATGCTGCTGGTGC
  TGGGTTTGTGCGGAGCCGCTTACCGGCCCACGGAGGAGATCGATCTGCGCAGCGTGGGCTGGGGCAACAT
  CTTCCAGCTGCCCTTCAAGCACGTGCGTGACTACCGCCTGCGCCACCTCGTGCCTTTCTTTATCTACAGC
  GGCTTCGAGGTGCTCTTTGCCTGCACTGGTATCGCCTTGGGCTATGGCGTGTGCTCGGTGGGGCTGGAGC
  GGCTGGCTTACCTCCTCGTGGCTTACAGCCTGGGCGCCTCAGCCGCCTCACTCCTGGGCCTGCTGGGCCT
  GTGGCTGCCACGCCCGGTGCCCCTGGTGGCCGGAGCAGGGGTGCACCTGCTGCTCACCTTCATCCTCTTT
  TTCTGGGCCCCTGTGCCTCGGGTCCTGCAACACAGCTGGATCCTCTATGTGGCAGCTGCCCTTTGGGGTG
  TGGGCAGTGCCCTGAACAAGACTGGACTCAGCACACTCCTGGGAATCTTGTACGAAGACAAGGAGAGACA
  GGACTTCATCTTCACCATCTACCACTGGTGGCAGGCTGTGGCCATCTTCACCGTGTACCTGGGCTCGAGC
  CTGCACATGAAGGCTAAGCTGGCGGTGCTGCTGGTGACGCTGGTGGCGGCCGCGGTCTCCTACCTGCGGA
  TGGAGCAGAAGCTGCGCCGGGGCGTGGCCCCGCGCCAGCCCCGCATCCCGCGGCCCCAGCACAAGGTGCG
  CGGTTACCGCTACTTGGAGGAGGACAACTCGGACGAGAGCGACGCGGAGGGCGAGCATGGGGACGGCGCG
  GAGGAGGAGGCGCCGCCCGCAGGGCCCAGGCCTGGCCCCGAGCCCGCTGGACTCGGCCGCCGGCCCTGCC
  CGTACGAACAGGCGCAGGGGGGAGACGGGCCGGAGGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210505 representing NM_030930
  Red=Cloning site Green=Tags(s)
  
  MEAEPPLYPMAGAAGPQGDEDLLGVPDGPEAPLDELVGAYPNYNEEEEERRYYRRKRLGVLKNVLAASAG
  GMLTYGVYLGLLQMQLILHYDETYREVKYGNMGLPDIDSKMLMGINVTPIAALLYTPVLIRFFGTKWMMF
  LAVGIYALFVSTNYWERYYTLVPSAVALGMAIVPLWASMGNYITRMAQKYHEYSHYKEQDGQGMKQRPPR
  GSHAPYLLVFQAIFYSFFHLSFACAQLPMIYFLNHYLYDLNHTLYNVQSCGTNSHGILSGFNKTVLRTLP
  RSGNLIVVESVLMAVAFLAMLLVLGLCGAAYRPTEEIDLRSVGWGNIFQLPFKHVRDYRLRHLVPFFIYS
  GFEVLFACTGIALGYGVCSVGLERLAYLLVAYSLGASAASLLGLLGLWLPRPVPLVAGAGVHLLLTFILF
  FWAPVPRVLQHSWILYVAAALWGVGSALNKTGLSTLLGILYEDKERQDFIFTIYHWWQAVAIFTVYLGSS
  LHMKAKLAVLLVTLVAAAVSYLRMEQKLRRGVAPRQPRIPRPQHKVRGYRYLEEDNSDESDAEGEHGDGA
  EEEAPPAGPRPGPEPAGLGRRPCPYEQAQGGDGPEEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030930
• ORF Size: 1791 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030930.4
• RefSeq Size: 2334 bp
• RefSeq ORF: 1794 bp
• Locus ID: 81622
• UniProt ID: Q9H1C4
• Cytogenetics: 11q13.2
• Protein Families: Transmembrane
• MW: 66.5 kDa
• Gene Summary: This gene encodes a protein that is involved in innate and adaptive immune response by regulating toll-like receptor signaling. The encoded protein traffics nucleotide sensing toll-like receptors to the endolysosome from the endoplasmic reticulum. Deficiency of the encoded protein has been associated with herpes simplex encephalitis. [provided by RefSeq, Feb 2014]