COP1 (RFWD2) (NM_022457) Human Tagged ORF Clone

CAT#: RC210492

RFWD2 (Myc-DDK-tagged)-Human ring finger and WD repeat domain 2 (RFWD2), transcript variant 1

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_022457" in other vectors (4)

Reconstitution Protocol

USD 669.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "COP1"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol COP1
Synonyms CFAP78; FAP78; RFWD2; RNF200
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210492 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGGTAGCCGCCAGGCCGGGTCGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCGG
TGACTTCCGCCTCCTCGTCTTTATCCTCTTCCCCGTCGCCGCCTTCCGTGGCGGTTTCGGCGGCAGCGCT
GGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGCCTCGGGGGCCCGGTGCGGCCTGTGTTGGTG
GCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCCCGGCACAGCTGCGCGGCCA
GGCCCAGCGCCGGCGTAGGAGGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGC
CCCCCTCTGCAACGGGCTCATCAACTCCTACGAGGACAAAAGCAACGACTTCGTATGCCCCATCTGCTTT
GATATGATTGAAGAAGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGTGTATTCATCAGAGTT
TGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATTGACCATCTGTATCCTAATTT
CTTGGTGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGACCACTCA
GTGAGTAGCACCAATGGCCACAGGTGGCAGATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTG
ATTTGGCCAATGTCAATCTTATGTTGGAGTTACTAGTGCAGAAGAAGAAACAACTGGAAGCAGAATCACA
TGCAGCCCAACTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAACTGGAA
CAGATCCAGAAGGAGCTAAGTGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGTGGCTTATACT
CTCCTGTCAGTGAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGTATTATTGA
TTCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGTTCTCAGACAAAGAAACAGCCTTGGTATAAT
AGCACGTTAGCATCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTA
CAAGGATGTCTCGTATCTCAGATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTC
CAAGTTTACTCGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGT
TCCAGTATAGTCTCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGA
AGATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGGATATTCATTACCCTGAGAATGAAAT
GACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCAGTGAT
TATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGA
AGAGGTGTTGGAGTGTTGACTTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAA
AGTGAAGCTGTGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGT
GTTAAATTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTCCACTACTATG
ATCTTCGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTT
TGTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTGTGGAATGTAGGGAAACCA
TACTGCCTACGTTCCTTCAAGGGTCATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATT
ATATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAAC
TTTTAAGTTTGATACAGTCAAAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTT
AGTGCTGTGTGCTGGAGGGCACTACCAGATGGGGAGTCCAATGTGCTGATTGCTGCTAACAGTCAGGGTA
CAATTAAGGTGCTAGAATTGGTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210492 protein sequence
Red=Cloning site Green=Tags(s)

MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLV
APAVSGSGGGAVSTGLSRHSCAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICF
DMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHS
VSSTNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLE
QIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYN
STLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNG
SSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSD
YEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCC
VKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKP
YCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFV
SAVCWRALPDGESNVLIAANSQGTIKVLELV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022457
ORF Size 2193 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022457.7
RefSeq Size 2806 bp
RefSeq ORF 2196 bp
Locus ID 64326
UniProt ID Q8NHY2
Cytogenetics 1q25.1-q25.2
Domains WD40, RING
Protein Pathways p53 signaling pathway, Ubiquitin mediated proteolysis
MW 80.5 kDa
Gene Summary E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).[UniProtKB/Swiss-Prot Function]

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