PSMD1 (NM_002807) Human Tagged ORF Clone

CAT#: RC210486

PSMD1 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1

Product Images

Circular map for RC210486

• 

  Western blot validation of overexpression lysate (Cat# LY400995) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210486 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSMD1
• Synonyms: P112; Rpn2; S1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210486 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCACCTCGGCCGCTGGAATTATTTCTCTTCTGGATGAAGATGAACCACAGCTTAAGGAATTTGCAC
  TACACAAATTGAATGCAGTTGTTAATGACTTCTGGGCAGAAATTTCCGAGTCCGTAGACAAAATAGAGGT
  TTTATACGAAGATGAAGGTTTCCGGAGTCGGCAGTTTGCAGCCTTAGTGGCATCTAAAGTATTTTATCAC
  CTGGGGGCTTTTGAGGAGTCTCTGAATTATGCTCTTGGAGCAGGGGACCTCTTCAATGTCAATGATAACT
  CTGAATATGTGGAAACTATTATAGCAAAATGCATTGATCACTACACCAAACAATGTGTGGAAAATGCAGA
  TTTGCCTGAAGGAGAAAAAAAACCAATTGACCAGAGATTGGAAGGCATCGTAAATAAAATGTTCCAGCGA
  TGTCTAGATGATCACAAGTATAAACAGGCTATTGGCATTGCTCTGGAGACACGAAGACTGGACGTCTTTG
  AAAAGACCATACTGGAGTCGAATGATGTCCCAGGAATGTTAGCTTATAGCCTTAAGCTCTGCATGTCTTT
  AATGCAGAATAAACAGTTTCGGAATAAAGTACTAAGAGTTCTAGTTAAAATCTACATGAACTTGGAGAAA
  CCTGATTTCATCAATGTTTGTCAGTGCTTAATTTTCTTAGATGATCCTCAGGCTGTGAGTGATATCTTAG
  AGAAACTGGTAAAGGAAGACAACCTCCTGATGGCATATCAGATTTGTTTTGATTTGTATGAAAGTGCTAG
  CCAGCAGTTTTTGTCATCTGTAATCCAGAATCTTCGAACTGTTGGCACCCCTATTGCTTCTGTGCCTGGA
  TCCACTAATACGGGTACTGTTCCGGGATCAGAGAAAGACAGTGACTCGATGGAAACAGAAGAAAAGACAA
  GCAGTGCATTTGTAGGAAAGACACCAGAAGCCAGTCCAGAGCCTAAGGACCAGACTTTGAAAATGATTAA
  AATTTTAAGTGGTGAAATGGCTATTGAGTTACATCTGCAGTTCTTAATACGAAACAATAATACAGACCTC
  ATGATTCTAAAAAACACAAAGGATGCAGTACGGAATTCTGTATGTCATACTGCAACCGTTATAGCAAACT
  CTTTTATGCACTGTGGGACAACCAGTGACCAGTTTCTTAGAGATAATTTGGAATGGTTAGCCAGAGCCAC
  TAACTGGGCAAAATTTACTGCTACAGCCAGTTTGGGTGTAATTCATAAGGGTCATGAAAAAGAAGCATTA
  CAGTTAATGGCAACATACCTTCCCAAGGATACTTCTCCAGGATCAGCCTATCAGGAAGGTGGAGGTCTCT
  ATGCACTAGGTCTTATTCATGCCAATCATGGTGGTGATATAATTGACTATCTGCTTAATCAGCTTAAGAA
  CGCCAGCAATGATATCGTTAGACACGGTGGCAGTCTGGGCCTTGGTTTGGCAGCCATGGGAACTGCACGT
  CAAGATGTTTATGATTTGCTAAAAACAAACCTTTATCAGGATGATGCAGTAACAGGGGAAGCAGCTGGCC
  TGGCCCTAGGTTTGGTTATGTTGGGCTCTAAAAATGCTCAGGCTATTGAGGACATGGTTGGTTATGCACA
  AGAAACTCAACATGAGAAGATTCTGCGTGGTCTTGCAGTTGGCATAGCTTTAGTAATGTATGGGAGGATG
  GAAGAGGCTGATGCTCTCATTGAATCTCTCTGTCGTGACAAGGACCCAATTCTTCGAAGGTCTGGAATGT
  ATACTGTAGCCATGGCTTATTGTGGCTCTGGTAACAACAAAGCAATTCGACGCCTGCTACATGTTGCTGT
  AAGTGATGTTAATGATGATGTCAGGAGGGCAGCAGTAGAATCACTTGGGTTCATTCTATTCAGAACCCCT
  GAACAGTGCCCAAGTGTTGTCTCTTTGTTGTCAGAGAGTTACAACCCTCATGTGCGCTACGGAGCTGCAA
  TGGCCTTGGGGATATGCTGTGCTGGTACAGGAAACAAGGAAGCCATTAATTTGCTAGAACCAATGACAAA
  CGACCCCGTGAACTACGTGAGGCAAGGGGCACTCATAGCTTCAGCTCTCATCATGATCCAGCAGACTGAA
  ATCACTTGTCCAAAGGTGAATCAGTTCAGACAGCTGTATTCCAAAGTCATCAATGATAAGCATGATGATG
  TCATGGCCAAGTTTGGCGCTATTCTGGCCCAGGGCATACTGGATGCAGGTGGTCATAATGTCACAATCTC
  CTTGCAGTCCAGGACTGGGCATACTCATATGCCTTCTGTGGTTGGCGTCCTTGTATTTACCCAGTTTTGG
  TTCTGGTTTCCTCTTTCACACTTCCTGTCATTGGCTTATACCCCTACCTGTGTCATTGGCCTTAACAAGG
  ACTTAAAGATGCCGAAAGTTCAGTATAAATCGAACTGTAAACCATCCACATTTGCATATCCTGCCCCTCT
  GGAAGTACCAAAAGAAAAAGAAAAGGAAAAGGTTTCTACTGCTGTATTATCTATAACTGCCAAGGCTAAA
  AAGAAGGAAAAAGAAAAGGAAAAAAAGGAGGAGGAGAAAATGGAAGTGGATGAGGCAGAGAAAAAGGAGG
  AAAAAGAGAAGAAAAAAGAACCTGAGCCAAACTTCCAGTTATTGGATAACCCAGCCCGAGTTATGCCTGC
  CCAGCTTAAGGTCCTAACCATGCCGGAGACCTGTAGATACCAGCCTTTCAAACCACTCTCTATTGGAGGC
  ATCATCATTCTGAAGGATACCAGTGAAGACATTGAGGAGCTGGTGGAACCTGTGGCAGCACATGGCCCAA
  AAATCGAGGAGGAGGAACAAGAGCCAGAACCCCCAGAACCATTTGAGTATATTGATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210486 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYH
  LGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQR
  CLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEK
  PDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPG
  STNTGTVPGSEKDSDSMETEEKTSSAFVGKTPEASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDL
  MILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEAL
  QLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTAR
  QDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVMYGRM
  EEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTP
  EQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTE
  ITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFW
  FWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAK
  KKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGG
  IIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002807
• ORF Size: 2859 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002807.4
• RefSeq Size: 3329 bp
• RefSeq ORF: 2862 bp
• Locus ID: 5707
• UniProt ID: Q99460
• Cytogenetics: 2q37.1
• Domains: PC_rep
• Protein Pathways: Proteasome
• MW: 105.8 kDa
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes the largest non-ATPase subunit of the 19S regulator lid, which is responsible for substrate recognition and binding. There is evidence that this proteasome and its subunits interact with viral proteins, including those of coronaviruses. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Aug 2020]