MC2 receptor (MC2R) (NM_000529) Human Tagged ORF Clone

CAT#: RC210483

Reviews ()
Write a review

MC2R (Myc-DDK-tagged)-Human melanocortin 2 receptor (adrenocorticotropic hormone) (MC2R)



  "NM_000529" in other vectors (6)

Reconstitution Protocol

USD 450.00


Availability*
In Stock

Size
    • 10 ug


Product images

Other products for "MC2R"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MC2R
Synonyms ACTHR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210483 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCACATTATCAACTCGTATGAAAACATCAACAACACAGCAAGAAATAATTCCGACTGTCCTCGTG
TGGTTTTGCCGGAGGAGATATTTTTCACAATTTCCATTGTTGGAGTTTTGGAGAATCTGATCGTCCTGCT
GGCTGTGTTCAAGAATAAGAATCTCCAGGCACCCATGTACTTTTTCATCTGTAGCTTGGCCATATCTGAT
ATGCTGGGCAGCCTATATAAGATCTTGGAAAATATCCTGATCATATTGAGAAACATGGGCTATCTCAAGC
CACGTGGCAGTTTTGAAACCACAGCCGATGACATCATCGACTCCCTGTTTGTCCTCTCCCTGCTTGGCTC
CATCTTCAGCCTGTCTGTGATTGCTGCGGACCGCTACATCACCATCTTCCACGCACTGCGGTACCACAGC
ATCGTGACCATGCGCCGCACTGTGGTGGTGCTTACGGTCATCTGGACGTTCTGCACGGGGACTGGCATCA
CCATGGTGATCTTCTCCCATCATGTGCCCACAGTGATCACCTTCACGTCGCTGTTCCCGCTGATGCTGGT
CTTCATCCTGTGCCTCTATGTGCACATGTTCCTGCTGGCTCGATCCCACACCAGGAAGATCTCCACCCTC
CCCAGAGCCAACATGAAAGGGGCCATCACACTGACCATCCTGCTCGGGGTCTTCATCTTCTGCTGGGCCC
CCTTTGTGCTTCATGTCCTCTTGATGACATTCTGCCCAAGTAACCCCTACTGCGCCTGCTACATGTCTCT
CTTCCAGGTGAACGGCATGTTGATCATGTGCAATGCCGTCATTGACCCCTTCATATATGCCTTCCGGAGC
CCAGAGCTCAGGGACGCATTCAAAAAGATGATCTTCTGCAGCAGGTACTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210483 protein sequence
Red=Cloning site Green=Tags(s)

MKHIINSYENINNTARNNSDCPRVVLPEEIFFTISIVGVLENLIVLLAVFKNKNLQAPMYFFICSLAISD
MLGSLYKILENILIILRNMGYLKPRGSFETTADDIIDSLFVLSLLGSIFSLSVIAADRYITIFHALRYHS
IVTMRRTVVVLTVIWTFCTGTGITMVIFSHHVPTVITFTSLFPLMLVFILCLYVHMFLLARSHTRKISTL
PRANMKGAITLTILLGVFIFCWAPFVLHVLLMTFCPSNPYCACYMSLFQVNGMLIMCNAVIDPFIYAFRS
PELRDAFKKMIFCSRYW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000529
ORF Size 891 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000529.2, NP_000520.1
RefSeq Size 3652 bp
RefSeq ORF 894 bp
Locus ID 4158
UniProt ID Q01718
Cytogenetics 18p11.21
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 33.9 kDa
Gene Summary MC2R encodes one member of the five-member G-protein associated melanocortin receptor family. Melanocortins (melanocyte-stimulating hormones and adrenocorticotropic hormone) are peptides derived from pro-opiomelanocortin (POMC). MC2R is selectively activated by adrenocorticotropic hormone, whereas the other four melanocortin receptors recognize a variety of melanocortin ligands. Mutations in MC2R can result in familial glucocorticoid deficiency. Alternate transcript variants have been found for this gene. [provided by RefSeq, May 2014]

Other Versions

Frequently bought together (4)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 177.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.