LZTS1 (NM_021020) Human Tagged ORF Clone

CAT#: RC210424

LZTS1 (Myc-DDK-tagged)-Human leucine zipper, putative tumor suppressor 1 (LZTS1)

Product Images

Circular map for RC210424

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LZTS1 (Cat# RC210424, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LZTS1 (Cat# TA810241)(1:2000). Positive lysates LY412145 (100ug) and LC412145 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412145) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210424 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LZTS1
• Synonyms: F37; FEZ1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210424 representing NM_021020
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCGTCAGTAGCCTCATCTCCGGCCACAGCTTCCACAGCAAGCACTGCCGGGCTTCGCAGTACA
  AGCTGCGCAAGTCCTCCCACCTCAAGAAGCTCAACCGGTATTCCGACGGGCTGCTGAGGTTTGGCTTCTC
  CCAGGACTCCGGTCACGGCAAGTCCAGCTCCAAAATGGGCAAGAGCGAAGACTTCTTCTACATCAAGGTC
  AGCCAGAAAGCCCGGGGCTCCCATCACCCAGATTACACGGCACTGTCCAGCGGGGATTTAGGGGGCCAGG
  CTGGGGTGGACTTTGACCCGTCCACACCCCCCAAGCTCATGCCCTTCTCCAATCAGCTAGAAATGGGCTC
  CGAGAAGGGTGCAGTGAGGCCCACAGCCTTCAAGCCTGTGCTGCCACGGTCAGGAGCCATCCTGCACTCC
  TCCCCGGAGAGTGCCAGCCACCAGCTGCACCCCGCCCCTCCAGACAAGCCCAAGGAGCAGGAGCTGAAGC
  CTGGCCTGTGCTCTGGGGCGCTGTCAGACTCCGGCCGGAACTCCATGTCCAGCCTGCCCACACACAGCAC
  CAGCAGCAGCTACCAGCTGGACCCGCTGGTCACACCCGTGGGACCCACAAGCCGTTTTGGGGGCTCCGCC
  CACAACATCACCCAGGGCATCGTCCTCCAGGACAGCAACATGATGAGCCTGAAGGCTCTGTCCTTCTCCG
  ACGGAGGTAGCAAGCTGGGCCACTCGAACAAGGCAGACAAGGGCCCCTCGTGTGTCCGCTCCCCCATCTC
  CACGGACGAGTGCAGCATCCAGGAGCTGGAGCAGAAGCTGTTGGAGAGGGAGGGCGCCCTCCAGAAGCTG
  CAGCGCAGCTTTGAGGAGAAGGAGCTTGCCTCCAGCCTGGCCTACGAGGAGCGGCCGCGGCGCTGCAGGG
  ACGAGCTGGAGGGCCCGGAGCCCAAAGGCGGCAACAAGCTCAAGCAGGCCTCGCAGAAGAGCCAGCGCGC
  GCAGCAGGTCCTGCACCTGCAGGTACTGCAGCTTCAGCAGGAGAAGCGGCAGCTCCGGCAGGAGCTCGAG
  AGCCTCATGAAGGAGCAGGACCTGCTGGAGACCAAGCTCAGGTCCTACGAGAGGGAGAAGACCAGCTTCG
  GCCCCGCGCTGGAGGAGACCCAGTGGGAGGTGTGCCAGAAGTCAGGCGAGATCTCCCTCCTGAAGCAGCA
  GCTGAAGGAGTCCCAGACGGAGGTGAACGCCAAGGCTAGCGAGATCCTGGGTCTCAAGGCACAGCTGAAG
  GACACGCGGGGCAAGCTGGAGGGCCTGGAGCTGAGGACCCAGGACCTGGAGGGCGCCCTGCGCACCAAGG
  GCCTGGAGCTGGAGGTCTGTGAGAATGAGCTGCAGCGCAAGAAGAACGAGGCGGAGCTGCTGCGGGAGAA
  GGTGAACCTGCTGGAGCAGGAGCTGCAGGAGCTGCGGGCCCAGGCCGCCCTGGCCCGCGACATGGGGCCG
  CCCACCTTCCCCGAGGACGTCCCTGCCCTGCAGCGGGAGCTGGAGCGGCTGCGGGCCGAGCTGCGGGAGG
  AGCGGCAAGGCCATGACCAGATGTCCTCGGGCTTCCAGCATGAGCGGCTCGTGTGGAAGGAGGAGAAGGA
  GAAGGTGATTCAGTACCAGAAACAGCTGCAGCAGAGCTACGTGGCCATGTACCAGCGGAACCAGCGCCTG
  GAGAAGGCCCTGCAGCAGCTGGCACGTGGGGACAGCGCCGGGGAGCCCTTGGAGGTTGACCTGGAAGGGG
  CTGACATCCCCTACGAGGACATCATAGCCACTGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210424 representing NM_021020
  Red=Cloning site Green=Tags(s)
  
  MGSVSSLISGHSFHSKHCRASQYKLRKSSHLKKLNRYSDGLLRFGFSQDSGHGKSSSKMGKSEDFFYIKV
  SQKARGSHHPDYTALSSGDLGGQAGVDFDPSTPPKLMPFSNQLEMGSEKGAVRPTAFKPVLPRSGAILHS
  SPESASHQLHPAPPDKPKEQELKPGLCSGALSDSGRNSMSSLPTHSTSSSYQLDPLVTPVGPTSRFGGSA
  HNITQGIVLQDSNMMSLKALSFSDGGSKLGHSNKADKGPSCVRSPISTDECSIQELEQKLLEREGALQKL
  QRSFEEKELASSLAYEERPRRCRDELEGPEPKGGNKLKQASQKSQRAQQVLHLQVLQLQQEKRQLRQELE
  SLMKEQDLLETKLRSYEREKTSFGPALEETQWEVCQKSGEISLLKQQLKESQTEVNAKASEILGLKAQLK
  DTRGKLEGLELRTQDLEGALRTKGLELEVCENELQRKKNEAELLREKVNLLEQELQELRAQAALARDMGP
  PTFPEDVPALQRELERLRAELREERQGHDQMSSGFQHERLVWKEEKEKVIQYQKQLQQSYVAMYQRNQRL
  EKALQQLARGDSAGEPLEVDLEGADIPYEDIIATEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021020
• ORF Size: 1788 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021020.5
• RefSeq Size: 5492 bp
• RefSeq ORF: 1791 bp
• Locus ID: 11178
• UniProt ID: Q9Y250
• Cytogenetics: 8p21.3
• Protein Families: Druggable Genome
• MW: 66.4 kDa
• Gene Summary: This gene encodes a tumor suppressor protein that is ubiquitously expressed in normal tissues. In uveal melanomas, expression of this protein is silenced in rapidly metastasizing and metastatic tumor cells but has normal expression in slowly metastasizing or nonmetastasizing tumor cells. This protein may have a role in cell-cycle control by interacting with the Cdk1/cyclinB1 complex. This gene is located on chromosomal region 8p22. Loss of heterozygosity (LOH) in the 8p arm is a common characteristic of many types of cancer. [provided by RefSeq, Nov 2009]