Myostatin Propeptide (MSTN) (NM_005259) Human Tagged ORF Clone

CAT#: RC210368

MSTN (Myc-DDK-tagged)-Human myostatin (MSTN)

Product Images

Circular map for RC210368

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MSTN (Cat# RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN (Cat# TA807791)(1:2000). Positive lysates LY401622 (100ug) and LC401622 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401622) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210368 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MSTN protein (Cat# TP310368). The protein was produced from HEK293T cells transfected with MSTN cDNA clone (Cat# RC210368) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myostatin Propeptide
• Synonyms: GDF8; MSLHP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210368 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAAACTGCAACTCTGTGTTTATATTTACCTGTTTATGCTGATTGTTGCTGGTCCAGTGGATCTAA
  ATGAGAACAGTGAGCAAAAAGAAAATGTGGAAAAAGAGGGGCTGTGTAATGCATGTACTTGGAGACAAAA
  CACTAAATCTTCAAGAATAGAAGCCATTAAGATACAAATCCTCAGTAAACTTCGTCTGGAAACAGCTCCT
  AACATCAGCAAAGATGTTATAAGACAACTTTTACCCAAAGCTCCTCCACTCCGGGAACTGATTGATCAGT
  ATGATGTCCAGAGGGATGACAGCAGCGATGGCTCTTTGGAAGATGACGATTATCACGCTACAACGGAAAC
  AATCATTACCATGCCTACAGAGTCTGATTTTCTAATGCAAGTGGATGGAAAACCCAAATGTTGCTTCTTT
  AAATTTAGCTCTAAAATACAATACAATAAAGTAGTAAAGGCCCAACTATGGATATATTTGAGACCCGTCG
  AGACTCCTACAACAGTGTTTGTGCAAATCCTGAGACTCATCAAACCTATGAAAGACGGTACAAGGTATAC
  TGGAATCCGATCTCTGAAACTTGACATGAACCCAGGCACTGGTATTTGGCAGAGCATTGATGTGAAGACA
  GTGTTGCAAAATTGGCTCAAACAACCTGAATCCAACTTAGGCATTGAAATAAAAGCTTTAGATGAGAATG
  GTCATGATCTTGCTGTAACCTTCCCAGGACCAGGAGAAGATGGGCTGAATCCGTTTTTAGAGGTCAAGGT
  AACAGACACACCAAAAAGATCCAGAAGGGATTTTGGTCTTGACTGTGATGAGCACTCAACAGAATCACGA
  TGCTGTCGTTACCCTCTAACTGTGGATTTTGAAGCTTTTGGATGGGATTGGATTATCGCTCCTAAAAGAT
  ATAAGGCCAATTACTGCTCTGGAGAGTGTGAATTTGTATTTTTACAAAAATATCCTCATACTCATCTGGT
  ACACCAAGCAAACCCCAGAGGTTCAGCAGGCCCTTGCTGTACTCCCACAAAGATGTCTCCAATTAATATG
  CTATATTTTAATGGCAAAGAACAAATAATATATGGGAAAATTCCAGCGATGGTAGTAGACCGCTGTGGGT
  GCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210368 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQKLQLCVYIYLFMLIVAGPVDLNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAP
  NISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQVDGKPKCCFF
  KFSSKIQYNKVVKAQLWIYLRPVETPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKT
  VLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESR
  CCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINM
  LYFNGKEQIIYGKIPAMVVDRCGCS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005259
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005259.3
• RefSeq Size: 2823 bp
• RefSeq ORF: 1128 bp
• Locus ID: 2660
• UniProt ID: O14793
• Cytogenetics: 2q32.2
• Protein Families: Druggable Genome, Secreted Protein
• MW: 42.8 kDa
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jul 2016]