PON1 (NM_000446) Human Tagged ORF Clone

CAT#: RC210356

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PON1 (Myc-DDK-tagged)-Human paraoxonase 1 (PON1)



  "NM_000446" in other vectors (6)

Reconstitution Protocol

USD 457.00


Availability*
In Stock

Size
    • 10 ug


Other products for "PON1"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PON1
Synonyms ESA; MVCD5; PON
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210356 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAAGCTGATTGCGCTCACCCTCTTGGGGATGGGACTGGCACTCTTCAGGAACCACCAGTCTTCTT
ACCAAACACGACTTAATGCTCTCCGAGAGGTACAACCCGTAGAACTTCCTAACTGTAATTTAGTTAAAGG
AATCGAAACTGGCTCTGAAGACTTGGAGATACTGCCTAATGGACTGGCTTTCATTAGCTCTGGATTAAAG
TATCCTGGAATAAAGAGCTTCAACCCCAACAGTCCTGGAAAAATACTTCTGATGGACCTGAATGAAGAAG
ATCCAACAGTGTTGGAATTGGGGATCACTGGAAGTAAATTTGATGTATCTTCATTTAACCCTCATGGGAT
TAGCACATTCACAGATGAAGATAATGCCATGTACCTCCTGGTGGTGAACCATCCAGATGCCAAGTCCACA
GTGGAGTTGTTTAAATTTCAAGAAGAAGAAAAATCGCTTTTGCATCTAAAAACCATCAGACATAAACTTC
TGCCTAATTTGAATGATATTGTTGCTGTGGGACCTGAGCACTTTTATGGCACAAATGATCACTATTTTCT
TGACCCCTACTTACAATCCTGGGAGATGTATTTGGGTTTAGCGTGGTCGTATGTTGTCTACTATAGTCCA
AGTGAAGTTCGAGTGGTGGCAGAAGGATTTGATTTTGCTAATGGAATCAACATTTCACCCGATGGCAAGT
ATGTCTATATAGCTGAGTTGCTGGCTCATAAGATTCATGTGTATGAAAAGCATGCTAATTGGACTTTAAC
TCCATTGAAGTCCCTTGACTTTAATACCCTCGTGGATAACATATCTGTGGATCCTGAGACAGGAGACCTT
TGGGTTGGATGCCATCCCAATGGCATGAAAATCTTCTTCTATGACTCAGAGAATCCTCCTGCATCAGAGG
TGCTTCGAATCCAGAACATTCTAACAGAAGAACCTAAAGTGACACAGGTTTATGCAGAAAATGGCACAGT
GTTGCAAGGCAGTACAGTTGCCTCTGTGTACAAAGGGAAACTGCTGATTGGCACAGTGTTTCACAAAGCT
CTTTACTGTGAGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210356 protein sequence
Red=Cloning site Green=Tags(s)

MAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLK
YPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKST
VELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWEMYLGLAWSYVVYYSP
SEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDL
WVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKA
LYCEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000446
ORF Size 1065 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000446.7
RefSeq Size 1769 bp
RefSeq ORF 1068 bp
Locus ID 5444
UniProt ID P27169
Cytogenetics 7q21.3
Domains Arylesterase
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Metabolic pathways
MW 39.7 kDa
Gene Summary This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7. [provided by RefSeq, Aug 2017]

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