SNRPB (NM_003091) Human Tagged ORF Clone

CAT#: RC210290

SNRPB (Myc-DDK-tagged)-Human small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 2

Product Images

Circular map for RC210290

• 

  Western blot validation of overexpression lysate (Cat# LY418902) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210290 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNRPB
• Synonyms: CCMS; COD; Sm-B/B'; SmB/B'; SmB/SmB'; snRNP-B; SNRPB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210290 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTGGGCAAGAGCAGCAAGATGCTGCAGCATATTGATTACAGGATGAGGTGCATCCTGCAGGACG
  GCCGGATCTTCATTGGCACCTTCAAGGCTTTTGACAAGCACATGAATTTGATCCTCTGTGACTGTGATGA
  GTTCAGAAAGATCAAGCCAAAGAACTCCAAACAAGCAGAAAGGGAAGAGAAGCGAGTCCTCGGTCTGGTG
  CTGCTGCGAGGGGAGAATCTGGTCTCAATGACAGTAGAGGGACCTCCTCCCAAAGATACTGGTATTGCTC
  GAGTTCCACTTGCTGGAGCTGCCGGGGGCCCAGGGATCGGCAGGGCTGCTGGCAGAGGAATCCCAGCTGG
  GGTTCCCATGCCCCAGGCTCCTGCAGGACTTGCTGGGCCAGTCCGTGGGGTTGGCGGGCCATCCCAACAG
  GTGATGACCCCACAAGGAAGAGGTACTGTTGCAGCCGCTGCAGCTGCTGCCACAGCCAGTATTGCCGGGG
  CTCCAACCCAGTACCCACCTGGCCGTGGGGGTCCTCCCCCACCTATGGGCCGAGGAGCACCCCCTCCAGG
  CATGATGGGCCCACCTCCTGGTATGAGACCTCCTATGGGTCCCCCAATGGGGATCCCCCCTGGAAGAGGG
  ACTCCAATGGGCATGCCCCCTCCGGGAATGCGGCCTCCTCCCCCTGGGATGCGAGGCCTTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210290 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLV
  LLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPMPQAPAGLAGPVRGVGGPSQQ
  VMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAPPPGMMGPPPGMRPPMGPPMGIPPGRG
  TPMGMPPPGMRPPPPGMRGLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003091
• ORF Size: 693 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003091.4
• RefSeq Size: 1153 bp
• RefSeq ORF: 696 bp
• Locus ID: 6628
• UniProt ID: P14678
• Cytogenetics: 20p13
• Domains: Sm
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Spliceosome, Systemic lupus erythematosus
• MW: 23.7 kDa
• Gene Summary: The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene. [provided by RefSeq, Jul 2008]