SLC10A1 (NM_003049) Human Tagged ORF Clone
SLC10A1 (Myc-DDK-tagged)-Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1)
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC210241 representing NM_003049
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210241 representing NM_003049
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-RsrII Cloning Scheme for this gene Plasmid Map|
|ORF Size||1047 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1580 bp|
|RefSeq ORF||1050 bp|
|Protein Families||Druggable Genome, Transmembrane|
|Gene Summary||The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis. [provided by RefSeq, Oct 2011]|
|cDNA Clone Resources|
|RC210241L1||Lenti ORF clone of Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1), Myc-DDK-tagged||
|RC210241L2||Lenti ORF clone of Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1), mGFP tagged||
|RC210241L3||Lenti ORF clone of Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1), Myc-DDK-tagged||
|RC210241L4||Lenti ORF clone of Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1), mGFP tagged||
|RG210241||SLC10A1 (GFP-tagged) - Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1)||
|SC118232||SLC10A1 (untagged)-Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1)||