SLC7A2 (NM_001008539) Human Tagged ORF Clone

CAT#: RC210214

SLC7A2 (Myc-DDK-tagged)-Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 (SLC7A2), transcript variant 2

Product Images

Circular map for RC210214

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  Western blot validation of overexpression lysate (Cat# LY422792) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210214 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SLC7A2 protein (Cat# TP310214). The protein was produced from HEK293T cells transfected with SLC7A2 cDNA clone (Cat# RC210214) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC7A2
• Synonyms: ATRC2; CAT2; HCAT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210214 representing NM_001008539
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCTTGCAGAGCCGCGCTGACCTTTGCCCGATGTCTGATCCGGAGAAAAATCGTGACCCTGGACA
  GTCTAGAAGACACCAAATTATGCCGCTGCTTATCCACCATGGACCTCATTGCCCTGGGCGTTGGAAGCAC
  CCTTGGGGCCGGGGTTTATGTCCTCGCTGGGGAGGTGGCCAAGGCAGACTCGGGCCCCAGCATCGTGGTG
  TCCTTCCTCATTGCTGCCCTGGCTTCAGTGATGGCTGGCCTCTGCTATGCCGAATTTGGGGCCCGTGTTC
  CCAAGACGGGGTCTGCATATTTGTACACCTACGTGACTGTCGGAGAGCTGTGGGCCTTCATCACTGGCTG
  GAATCTCATTTTATCGTATGTGATAGGTACATCAAGTGTTGCAAGAGCCTGGAGTGGCACCTTTGATGAA
  CTTCTTAGCAAACAGATTGGTCAGTTTTTGAGGACATACTTCAGAATGAATTACACTGGTCTTGCAGAAT
  ATCCCGATTTTTTTGCTGTGTGCCTTATATTACTTCTAGCAGGTCTTTTGTCTTTTGGAGTAAAAGAGTC
  TGCTTGGGTGAATAAAGTCTTCACAGCTGTTAATATTCTCGTCCTTCTGTTTGTGATGGTTGCTGGGTTT
  GTGAAAGGAAATGTGGCAAACTGGAAGATTAGTGAAGAGTTTCTCAAAAATATATCAGCAAGTGCCAGAG
  AGCCACCTTCTGAAAACGGAACAAGTATCTATGGGGCTGGTGGCTTTATGCCTTATGGCTTTACGGGAAC
  GTTGGCTGGTGCTGCAACTTGCTTTTATGCCTTTGTGGGATTTGACTGCATTGCAACAACTGGTGAAGAA
  GTTCGGAATCCCCAGAAAGCTATTCCCATTGGAATTGTGACGTCTTTGCTTGTTTGCTTTATGGCCTATT
  TTGGGGTCTCTGCAGCTTTAACACTTATGATGCCGTACTACCTCCTCGATGAAAAAAGCCCCCTTCCTGT
  AGCGTTTGAATATGTGGGATGGGGTCCTGCCAAATATGTCGTCGCAGCTGGTTCTCTCTGCGCCTTGTCA
  ACAAGTCTTCTTGGATCCATTTTCCCAATGCCTCGTGTAATCTATGCTATGGCGGAGGATGGGTTGCTTT
  TCAAATGTCTAGCTCAAATCAATTCCAAAACGAAGACACCAATAATTGCTACTTTATCATCGGGTGCAGT
  GGCAGCTTTGATGGCCTTTCTGTTTGACCTGAAGGCGCTTGTGGACATGATGTCCATTGGCACCCTCATG
  GCCTACTCTCTGGTGGCAGCCTGTGTTCTCATCCTCAGGTACCAGCCTGGCTTATCTTACGACCAGCCCA
  AATGTTCTCCTGAGAAAGATGGTCTGGGATCGTCTCCCAGGGTAACCTCGAAGAGTGAGTCCCAGGTCAC
  CATGCTGCAGAGACAGGGCTTCAGCATGCGGACCCTCTTCTGCCCCTCCCTTCTGCCAACACAGCAGTCA
  GCTTCTCTCGTGAGCTTTCTGGTAGGATTCCTAGCTTTCCTCGTGTTGGGCCTGAGTGTCTTGACCACTT
  ACGGAGTTCATGCCATCACCAGGCTGGAGGCCTGGAGCCTCGCTCTCCTCGCGCTGTTTCTTGTTCTCTT
  CGTTGCCATCGTTCTCACCATCTGGAGGCAGCCCCAGAATCAGCAAAAAGTAGCCTTCATGGTTCCATTC
  TTACCATTTTTGCCAGCGTTCAGCATCTTGGTGAACATTTACTTGATGGTCCAGTTAAGTGCAGACACTT
  GGGTCAGATTCAGCATTTGGATGGCAATTGGCTTCCTGATTTACTTTTCTTATGGCATTAGACACAGCCT
  GGAGGGTCATCTGAGAGATGAAAACAATGAAGAAGATGCTTATCCAGACAACGTTCATGCAGCAGCAGAA
  GAAAAATCTGCCATTCAAGCAAATGACCATCACCCAAGAAATCTCAGTTCACCTTTCATATTCCATGAAA
  AGACAAGTGAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210214 representing NM_001008539
  Red=Cloning site Green=Tags(s)
  
  MIPCRAALTFARCLIRRKIVTLDSLEDTKLCRCLSTMDLIALGVGSTLGAGVYVLAGEVAKADSGPSIVV
  SFLIAALASVMAGLCYAEFGARVPKTGSAYLYTYVTVGELWAFITGWNLILSYVIGTSSVARAWSGTFDE
  LLSKQIGQFLRTYFRMNYTGLAEYPDFFAVCLILLLAGLLSFGVKESAWVNKVFTAVNILVLLFVMVAGF
  VKGNVANWKISEEFLKNISASAREPPSENGTSIYGAGGFMPYGFTGTLAGAATCFYAFVGFDCIATTGEE
  VRNPQKAIPIGIVTSLLVCFMAYFGVSAALTLMMPYYLLDEKSPLPVAFEYVGWGPAKYVVAAGSLCALS
  TSLLGSIFPMPRVIYAMAEDGLLFKCLAQINSKTKTPIIATLSSGAVAALMAFLFDLKALVDMMSIGTLM
  AYSLVAACVLILRYQPGLSYDQPKCSPEKDGLGSSPRVTSKSESQVTMLQRQGFSMRTLFCPSLLPTQQS
  ASLVSFLVGFLAFLVLGLSVLTTYGVHAITRLEAWSLALLALFLVLFVAIVLTIWRQPQNQQKVAFMVPF
  LPFLPAFSILVNIYLMVQLSADTWVRFSIWMAIGFLIYFSYGIRHSLEGHLRDENNEEDAYPDNVHAAAE
  EKSAIQANDHHPRNLSSPFIFHEKTSEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008539
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008539.4
• RefSeq Size: 7389 bp
• RefSeq ORF: 1977 bp
• Locus ID: 6542
• UniProt ID: P52569
• Cytogenetics: 8p22
• Protein Families: Druggable Genome, Transmembrane
• MW: 71.5 kDa
• Gene Summary: The protein encoded by this gene is a cationic amino acid transporter and a member of the APC (amino acid-polyamine-organocation) family of transporters. The encoded membrane protein is responsible for the cellular uptake of arginine, lysine and ornithine. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]