p53 AIP1 (TP53AIP1) (NM_022112) Human Tagged ORF Clone

CAT#: RC210110

TP53AIP1 (Myc-DDK-tagged)-Human tumor protein p53 regulated apoptosis inducing protein 1 (TP53AIP1), transcript variant 1

Product Images

Circular map for RC210110

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  Western blot validation of overexpression lysate (Cat# LY411784) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210110 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: p53 AIP1
• Synonyms: P53AIP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210110 representing NM_022112
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATCTTCCTCTGAGGCGAGCTTCAGATCTGCTCAAGCTTCCTGCAGTGGGGCCAGGAGGCAGGGCC
  TGGGCAGGGGAGACCAGAACCTCTCGGTGATGCCTCCGAATGGCAGGGCTCAGACACACACACCTGGCTG
  GGTTTCAGATCCCTTAGTTTTGGGTGCCCAAGTTCACGGAGGGTGCCGGGGAATAGAAGCTCTGTCAGTC
  TCGTCTGGATCTTGGTCCTCAGCAACTGTCTGGATCCTGACAGGCCTTGGTCTAGGTCTCTCCAGGCCTT
  TCCTTCCTGGAGCCACAGTGCTTAGAGACAGGCCACTGGGGTCAGCATTTGAGCTCAGCTATGATCAGAA
  AAAAGCACCGTTGAGGTTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210110 representing NM_022112
  Red=Cloning site Green=Tags(s)
  
  MGSSSEASFRSAQASCSGARRQGLGRGDQNLSVMPPNGRAQTHTPGWVSDPLVLGAQVHGGCRGIEALSV
  SSGSWSSATVWILTGLGLGLSRPFLPGATVLRDRPLGSAFELSYDQKKAPLRLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022112
• ORF Size: 372 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022112.2, NP_071395.2
• RefSeq Size: 1278 bp
• RefSeq ORF: 375 bp
• Locus ID: 63970
• UniProt ID: Q9HCN2
• Cytogenetics: 11q24.3
• Protein Families: Druggable Genome
• Protein Pathways: p53 signaling pathway
• MW: 12.9 kDa
• Gene Summary: This gene is specifically expressed in the thymus, and encodes a protein that is localized to the mitochondrion. The expression of this gene is inducible by p53, and it is thought to play an important role in mediating p53-dependent apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]