KLF2 (NM_016270) Human Tagged ORF Clone

CAT#: RC210042

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KLF2 (Myc-DDK-tagged)-Human Kruppel-like factor 2 (lung) (KLF2)



  "NM_016270" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KLF2
Synonyms LKLF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210042 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGAGTGAACCCATCCTGCCGTCCTTCTCCACTTTCGCCAGCCCGTGCCGCGAGCGCGGCCTGC
AGGAGCGCTGGCCGCGCGCCGAACCCGAGTCCGGCGGCACCGACGACGACCTCAACAGCGTGCTGGACTT
CATCCTGTCCATGGGGCTGGATGGCCTGGGCGCCGAGGCCGCCCCGGAGCCGCCGCCGCCGCCCCCGCCG
CCTGCGTTCTATTACCCCGAACCCGGCGCGCCCCCGCCCTACAGCGCCCCCGCGGGTGGCCTGGTGTCTG
AGCTGCTGCGACCCGAGCTGGATGCGCCGCCGGGGCCCGCACTGCACGGCCGCTTTCTGCTGGCGCCGCC
CGGCCGCCTGGTCAAGGCCGAGCCCCCTGAAGCGGACGGCGGCGGCGGCTACGGCTGCGCCCCCGGGCTG
ACCCGTGGACCGCGCGGCCTCAAGCGCGAGGGCGCCCCGGGCCCGGCGGCTTCGTGCATGCGAGGTCCCG
GGGGCCGCCCCCCGCCGCCGCCCGACACACCGCCGCTCAGCCCCGACGGCCCCGCGCGCCTGCCCGCGCC
CGGTCCGCGCGCCTCCTTCCCGCCGCCTTTCGGTGGCCCTGGTTTCGGCGCGCCCGGGCCCGGCCTGCAT
TACGCGCCGCCTGCGCCCCCAGCCTTCGGTCTCTTCGACGACGCGGCCGCCGCCGCGGCAGCCCTGGGCC
TGGCGCCCCCCGCCGCCCGCGGTCTCCTCACGCCGCCTGCGTCCCCGCTGGAGCTGCTGGAGGCCAAGCC
AAAGCGCGGCCGCCGCTCTTGGCCCCGCAAACGCACCGCCACTCACACCTGCAGCTACGCGGGCTGCGGC
AAGACCTACACCAAGAGTTCGCATCTGAAGGCGCATCTGCGCACGCACACAGGTGAGAAGCCCTACCACT
GCAACTGGGACGGCTGCGGCTGGAAGTTTGCGCGCTCAGACGAGCTCACGCGCCACTACCGAAAGCACAC
GGGCCACCGGCCATTCCAGTGCCATCTGTGCGATCGTGCCTTCTCGCGCTCCGATCACCTGGCGCTGCAC
ATGAAACGGCACATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210042 protein sequence
Red=Cloning site Green=Tags(s)

MALSEPILPSFSTFASPCRERGLQERWPRAEPESGGTDDDLNSVLDFILSMGLDGLGAEAAPEPPPPPPP
PAFYYPEPGAPPPYSAPAGGLVSELLRPELDAPPGPALHGRFLLAPPGRLVKAEPPEADGGGGYGCAPGL
TRGPRGLKREGAPGPAASCMRGPGGRPPPPPDTPPLSPDGPARLPAPGPRASFPPPFGGPGFGAPGPGLH
YAPPAPPAFGLFDDAAAAAAALGLAPPAARGLLTPPASPLELLEAKPKRGRRSWPRKRTATHTCSYAGCG
KTYTKSSHLKAHLRTHTGEKPYHCNWDGCGWKFARSDELTRHYRKHTGHRPFQCHLCDRAFSRSDHLALH
MKRHM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016270
ORF Size 1065 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016270.4
RefSeq Size 1655 bp
RefSeq ORF 1068 bp
Locus ID 10365
UniProt ID Q9Y5W3
Cytogenetics 19p13.11
Domains zf-C2H2
Protein Families Transcription Factors
MW 37.4 kDa
Gene Summary This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is expressed early in mammalian development and is found in many different cell types. The protein acts to bind the CACCC box found in the promoter of target genes to activate their transcription. It plays a role in many processes during development and disease including adipogenesis, embryonic erythropoiesis, epithelial integrity, inflammation and t-cell viability. [provided by RefSeq, Mar 2017]

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