KLF2 (NM_016270) Human Tagged ORF Clone

CAT#: RC210042

KLF2 (Myc-DDK-tagged)-Human Kruppel-like factor 2 (lung) (KLF2)

Product Images

Circular map for RC210042

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KLF2 (Cat# RC210042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLF2(Cat# TA807009). Positive lysates LY414082 (100ug) and LC414082 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY414082) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210042 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KLF2 protein (Cat# TP310042). The protein was produced from HEK293T cells transfected with KLF2 cDNA clone (Cat# RC210042) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLF2
• Synonyms: LKLF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210042 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGAGTGAACCCATCCTGCCGTCCTTCTCCACTTTCGCCAGCCCGTGCCGCGAGCGCGGCCTGC
  AGGAGCGCTGGCCGCGCGCCGAACCCGAGTCCGGCGGCACCGACGACGACCTCAACAGCGTGCTGGACTT
  CATCCTGTCCATGGGGCTGGATGGCCTGGGCGCCGAGGCCGCCCCGGAGCCGCCGCCGCCGCCCCCGCCG
  CCTGCGTTCTATTACCCCGAACCCGGCGCGCCCCCGCCCTACAGCGCCCCCGCGGGTGGCCTGGTGTCTG
  AGCTGCTGCGACCCGAGCTGGATGCGCCGCCGGGGCCCGCACTGCACGGCCGCTTTCTGCTGGCGCCGCC
  CGGCCGCCTGGTCAAGGCCGAGCCCCCTGAAGCGGACGGCGGCGGCGGCTACGGCTGCGCCCCCGGGCTG
  ACCCGTGGACCGCGCGGCCTCAAGCGCGAGGGCGCCCCGGGCCCGGCGGCTTCGTGCATGCGAGGTCCCG
  GGGGCCGCCCCCCGCCGCCGCCCGACACACCGCCGCTCAGCCCCGACGGCCCCGCGCGCCTGCCCGCGCC
  CGGTCCGCGCGCCTCCTTCCCGCCGCCTTTCGGTGGCCCTGGTTTCGGCGCGCCCGGGCCCGGCCTGCAT
  TACGCGCCGCCTGCGCCCCCAGCCTTCGGTCTCTTCGACGACGCGGCCGCCGCCGCGGCAGCCCTGGGCC
  TGGCGCCCCCCGCCGCCCGCGGTCTCCTCACGCCGCCTGCGTCCCCGCTGGAGCTGCTGGAGGCCAAGCC
  AAAGCGCGGCCGCCGCTCTTGGCCCCGCAAACGCACCGCCACTCACACCTGCAGCTACGCGGGCTGCGGC
  AAGACCTACACCAAGAGTTCGCATCTGAAGGCGCATCTGCGCACGCACACAGGTGAGAAGCCCTACCACT
  GCAACTGGGACGGCTGCGGCTGGAAGTTTGCGCGCTCAGACGAGCTCACGCGCCACTACCGAAAGCACAC
  GGGCCACCGGCCATTCCAGTGCCATCTGTGCGATCGTGCCTTCTCGCGCTCCGATCACCTGGCGCTGCAC
  ATGAAACGGCACATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210042 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALSEPILPSFSTFASPCRERGLQERWPRAEPESGGTDDDLNSVLDFILSMGLDGLGAEAAPEPPPPPPP
  PAFYYPEPGAPPPYSAPAGGLVSELLRPELDAPPGPALHGRFLLAPPGRLVKAEPPEADGGGGYGCAPGL
  TRGPRGLKREGAPGPAASCMRGPGGRPPPPPDTPPLSPDGPARLPAPGPRASFPPPFGGPGFGAPGPGLH
  YAPPAPPAFGLFDDAAAAAAALGLAPPAARGLLTPPASPLELLEAKPKRGRRSWPRKRTATHTCSYAGCG
  KTYTKSSHLKAHLRTHTGEKPYHCNWDGCGWKFARSDELTRHYRKHTGHRPFQCHLCDRAFSRSDHLALH
  MKRHM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016270
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016270.4
• RefSeq Size: 1655 bp
• RefSeq ORF: 1068 bp
• Locus ID: 10365
• UniProt ID: Q9Y5W3
• Cytogenetics: 19p13.11
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• MW: 37.4 kDa
• Gene Summary: This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is expressed early in mammalian development and is found in many different cell types. The protein acts to bind the CACCC box found in the promoter of target genes to activate their transcription. It plays a role in many processes during development and disease including adipogenesis, embryonic erythropoiesis, epithelial integrity, inflammation and t-cell viability. [provided by RefSeq, Mar 2017]