HAP40 (F8A1) (NM_012151) Human Tagged ORF Clone

CAT#: RC210039

F8A1 (Myc-DDK-tagged)-Human coagulation factor VIII-associated 1 (F8A1)

Product Images

Circular map for RC210039

• 

  Western blot validation of overexpression lysate (Cat# LY415937) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210039 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HAP40
• Synonyms: DXS522E; F8A; HAP40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210039 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGCGGCTGCAGGCCTGGGCGGCGGCGGCGCCGGCCCGGGACCCGAGGCCGGGGACTTCCTGG
  CCCGCTACCGGCTGGTATCGAACAAGCTGAAGAAGCGGTTCCTGCGGAAGCCGAACGTGGCGGAGGCCGG
  CGAGCAGTTCGGACAGCTGGGCCGGGAGCTGCGCGCCCAGGAGTGTCTGCCCTACGCGGCCTGGTGCCAG
  CTGGCGGTGGCGCGCTGCCAGCAGGCGCTCTTCCACGGGCCCGGGGAGGCGCTGGCCCTCACCGAGGCCG
  CCCGCCTCTTCCTGCGGCAGGAGCGCGACGCGCGCCAGCGCCTGGTCTGCCCCGCCGCCTACGGGGAGCC
  GCTGCAGGCCGCCGCCAGCGCCCTGGGCGCCGCGGTGCGTCTGCACCTCGAGCTGGGCCAGCCGGCCGCC
  GCCGCCGCCCTCTGCCTCGAGCTGGCCGCCGCCCTGCGCGACCTGGGCCAGCCGGCCGCCGCCGCCGGTC
  ACTTCCAGCGCGCCGCCCAGCTCCAGCTGCCCCAGCTGCCCCTGGCCGCGCTGCAGGCGCTTGGCGAGGC
  CGCCTCCTGCCAGCTGCTGGCGCGCGACTACACCGGCGCCCTGGCGGTCTTCACGCGCATGCAGCGCCTG
  GCGCGGGAGCACGGCAGCCACCCGGTGCAGTCACTGCCGCCGCCCCCGCCGCCGGCACCCCAGCCCGGGC
  CCGGGGCGACGCCCGCCCTACCGGCCGCGCTGCTTCCTCCGAACTCCGGCTCGGCGGCGCCCTCTCCCGC
  CGCCCTGGGCGCCTTCTCGGACGTGCTGGTCCGCTGCGAGGTGTCCCGCGTGCTGCTGCTGCTCCTCCTG
  CAACCACCGCCCGCCAAGCTGCTGCCGGAGCACGCCCAGACCCTGGAGAAGTACTCCTGGGAGGCTTTTG
  ACAGCCACGGGCAGGAGAGCAGCGGCCAGCTTCCCGAGGAGCTCTTTCTGCTGCTCCAGTCTTTGGTCAT
  GGCTACCCACGAAAAGGACACGGAAGCCATCAAGTCGCTGCAGGTGGAGATGTGGCCACTGTTGACTGCT
  GAGCAGAACCACCTCCTTCACCTCGTTCTGCAAGAAACCATCTCCCCCTCAGGACAGGGAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210039 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAAAGLGGGGAGPGPEAGDFLARYRLVSNKLKKRFLRKPNVAEAGEQFGQLGRELRAQECLPYAAWCQ
  LAVARCQQALFHGPGEALALTEAARLFLRQERDARQRLVCPAAYGEPLQAAASALGAAVRLHLELGQPAA
  AAALCLELAAALRDLGQPAAAAGHFQRAAQLQLPQLPLAALQALGEAASCQLLARDYTGALAVFTRMQRL
  AREHGSHPVQSLPPPPPPAPQPGPGATPALPAALLPPNSGSAAPSPAALGAFSDVLVRCEVSRVLLLLLL
  QPPPAKLLPEHAQTLEKYSWEAFDSHGQESSGQLPEELFLLLQSLVMATHEKDTEAIKSLQVEMWPLLTA
  EQNHLLHLVLQETISPSGQGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012151
• ORF Size: 1113 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012151.3, NP_036283.2
• RefSeq Size: 1720 bp
• RefSeq ORF: 1116 bp
• Locus ID: 8263
• UniProt ID: P23610
• Cytogenetics: Xq28
• MW: 39.1 kDa
• Gene Summary: This gene is contained entirely within intron 22 of the factor VIII gene; spans less than 2 kb, and is transcribed in the direction opposite of factor VIII. A portion of intron 22 (int22h), containing F8A, is repeated twice extragenically closer to the Xq telomere. Although its function is unknown, the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII, this gene is transcribed abundantly in a wide variety of cell types. [provided by RefSeq, Jul 2008]