FAM111A (NM_022074) Human Tagged ORF Clone

CAT#: RC210012

FAM111A (Myc-DDK-tagged)-Human family with sequence similarity 111, member A (FAM111A), transcript variant 1

Product Images

Circular map for RC210012

• 

  Western blot validation of overexpression lysate (Cat# LY411797) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210012 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM111A
• Synonyms: GCLEB; KCS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210012 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGTAAGAAGCAGAGGTCACGGAAGCACTCAGTCAATGAAAAATGTAATATGAAAATCGAGCACT
  ATTTTTCTCCGGTCTCTAAAGAGCAACAGAATAATTGCAGTACTTCTCTAATGAGGATGGAGTCTAGAGG
  AGACCCAAGAGCCACAACTAATACCCAGGCTCAAAGATTCCATTCACCTAAGAAAAATCCAGAAGACCAG
  ACCATGCCCCAAAATAGGACAATATATGTTACCTTGAAGGTAAACCACAGGAGAAACCAAGATATGAAAC
  TTAAGCTCTCACATAGTGAGAATAGTAGCTTATATATGGCTCTCAACACTCTCCAGGCTGTCAGAAAAGA
  GATAGAAACTCACCAAGGCCAAGAAATGCTTGTGCGTGGCACAGAAGGAATCAAAGAGTACATAAACCTT
  GGAATGCCCCTCAGTTGTTTCCCTGAAGGTGGCCAGGTGGTCATTACATTTTCCCAAAGTAAAAGTAAGC
  AGAAGGAAGATAACCACATATTTGGCAGGCAGGACAAAGCATCGACTGAATGTGTCAAATTTTACATTCA
  TGCAATTGGAATTGGGAAGTGTAAAAGAAGGATTGTTAAATGTGGGAAGCTTCACAAAAAGGGGCGCAAA
  CTCTGTGTTTATGCTTTCAAAGGAGAAACCATCAAGGATGCACTGTGCAAGGATGGCAGATTTCTTTCCT
  TTCTGGAGAATGATGATTGGAAACTCATTGAAAACAATGACACCATTTTAGAAAGCACCCAGCCAGTTGA
  TGAATTAGAAGGCAGATACTTTCAGGTTGAGGTTGAGAAAAGAATGGTCCCCAGTGCAGCAGCTTCTCAG
  AATCCTGAGTCAGAGAAAAGAAACACCTGTGTGTTGAGAGAACAAATCGTGGCTCAGTACCCCAGTTTGA
  AAAGAGAAAGTGAAAAAATCATTGAAAACTTCAAGAAAAAAATGAAAGTAAAAAATGGGGAAACATTATT
  TGAATTGCATAGAACAACGTTTGGGAAAGTAACAAAAAATTCTTCTTCGATTAAAGTAGTGAAACTTCTT
  GTACGTCTCAGTGACTCAGTTGGGTACTTATTCTGGGACAGTGCAACTACGGGTTACGCCACCTGCTTTG
  TTTTTAAAGGATTGTTCATTTTAACTTGTCGGCATGTAATAGATAGCATTGTGGGAGACGGAATAGAGCC
  AAGTAAGTGGGCAACCATAATTGGTCAATGTGTAAGGGTGACATTTGGTTATGAAGAGCTAAAAGACAAG
  GAAACAAACTACTTTTTTGTTGAACCTTGGTTTGAGATACATAATGAAGAGCTTGACTATGCTGTCCTGA
  AACTGAAGGAAAATGGACAACAAGTACCTATGGAACTATATAATGGAATTACTCCTGTGCCACTTAGTGG
  GTTGATACATATTATTGGCCATCCATATGGAGAAAAAAAGCAGATTGATGCTTGTGCTGTGATCCCTCAG
  GGTCAGCGAGCAAAGAAATGTCAGGAACGTGTTCAGTCTAAAAAAGCAGAAAGTCCAGAGTATGTCCATA
  TGTATACTCAAAGAAGTTTCCAGAAAATAGTTCACAACCCTGATGTGATTACCTATGACACTGAATTTTT
  CTTTGGGGCTTCCGGCTCCCCTGTGTTTGATTCAAAAGGTTCATTGGTGGCCATGCATGCTGCTGGCTTT
  GCTTATACTTACCAAAATGAGACTCGTAGTATCATTGAGTTTGGCTCTACCATGGAATCCATCCTCCTTG
  ATATTAAGCAAAGACATAAACCATGGTATGAAGAAGTATTTGTAAATCAGCAGGATGTAGAAATGATGAG
  TGATGAGGACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210012 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSCKKQRSRKHSVNEKCNMKIEHYFSPVSKEQQNNCSTSLMRMESRGDPRATTNTQAQRFHSPKKNPEDQ
  TMPQNRTIYVTLKVNHRRNQDMKLKLSHSENSSLYMALNTLQAVRKEIETHQGQEMLVRGTEGIKEYINL
  GMPLSCFPEGGQVVITFSQSKSKQKEDNHIFGRQDKASTECVKFYIHAIGIGKCKRRIVKCGKLHKKGRK
  LCVYAFKGETIKDALCKDGRFLSFLENDDWKLIENNDTILESTQPVDELEGRYFQVEVEKRMVPSAAASQ
  NPESEKRNTCVLREQIVAQYPSLKRESEKIIENFKKKMKVKNGETLFELHRTTFGKVTKNSSSIKVVKLL
  VRLSDSVGYLFWDSATTGYATCFVFKGLFILTCRHVIDSIVGDGIEPSKWATIIGQCVRVTFGYEELKDK
  ETNYFFVEPWFEIHNEELDYAVLKLKENGQQVPMELYNGITPVPLSGLIHIIGHPYGEKKQIDACAVIPQ
  GQRAKKCQERVQSKKAESPEYVHMYTQRSFQKIVHNPDVITYDTEFFFGASGSPVFDSKGSLVAMHAAGF
  AYTYQNETRSIIEFGSTMESILLDIKQRHKPWYEEVFVNQQDVEMMSDEDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022074
• ORF Size: 1833 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022074.4
• RefSeq Size: 3609 bp
• RefSeq ORF: 1836 bp
• Locus ID: 63901
• UniProt ID: Q96PZ2
• Cytogenetics: 11q12.1
• MW: 70.2 kDa
• Gene Summary: The protein encoded by this gene is cell-cycle regulated, and has nuclear localization. The C-terminal half of the protein shares homology with trypsin-like peptidases and it contains a PCNA-interacting peptide (PIP) box, that is necessary for its co-localization with proliferating cell nuclear antigen (PCNA). Reduced expression of this gene resulted in DNA replication defects, consistent with the demonstrated role for this gene in Simian Virus 40 (SV40) viral replication. Mutations in this gene have been associated with Kenny-Caffey syndrome (KCS) type 2 and the more severe osteocraniostenosis (OCS, also known as Gracile Bone Dysplasia), both characterized by short stature, hypoparathyroidism, bone development abnormalities, and hypocalcemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]