Ribosomal protein L26 (RPL26) (NM_000987) Human Tagged ORF Clone
RPL26 (Myc-DDK-tagged)-Human ribosomal protein L26 (RPL26)
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC209922 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC209922 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||435 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||602 bp|
|RefSeq ORF||438 bp|
|Gene Summary||Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L24P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Mutations in this gene result in Diamond-Blackfan anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]|
|cDNA Clone Resources|
|RC209922L1||Lenti ORF clone of Human ribosomal protein L26 (RPL26), Myc-DDK-tagged||
|RC209922L2||Lenti ORF clone of Human ribosomal protein L26 (RPL26), mGFP tagged||
|RC209922L3||Lenti ORF clone of Human ribosomal protein L26 (RPL26), Myc-DDK-tagged||
|RC209922L4||Lenti ORF clone of Human ribosomal protein L26 (RPL26), mGFP tagged||
|RG209922||RPL26 (GFP-tagged) - Human ribosomal protein L26 (RPL26)||
|SC119493||RPL26 (untagged)-Human ribosomal protein L26 (RPL26)||
|SC321975||RPL26 (untagged)-Human ribosomal protein L26 (RPL26)||