RETREG1 (NM_019000) Human Tagged ORF Clone

CAT#: RC209916

FAM134B (Myc-DDK-tagged)-Human family with sequence similarity 134, member B (FAM134B), transcript variant 2

Product Images

Circular map for RC209916

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  Western blot validation of overexpression lysate (Cat# LY412838) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209916 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RETREG1 protein (Cat# TP309916). The protein was produced from HEK293T cells transfected with RETREG1 cDNA clone (Cat# RC209916) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RETREG1
• Synonyms: FAM134B; JK-1; JK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209916 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAAGGTGAAGACTTTGGACCAGGCAAAAGCTGGGAAGTTATCAATTCCAAACCAGATGAAAGAC
  CCAGGCTCAGCCACTGTATTGCAGAATCATGGATGAATTTCAGCATATTTCTTCAAGAAATGTCTCTTTT
  TAAACAGCAGAGCCCTGGCAAGTTTTGTCTCCTGGTCTGTAGTGTGTGCACATTTTTTACGATCTTGGGA
  AGTTACATTCCTGGGGTTATACTCAGCTATCTACTGTTACTGTGTGCATTTTTGTGTCCATTGTTTAAAT
  GTAATGATATTGGACAAAAAATTTACAGCAAAATTAAGTCAGTTCTGCTGAAACTGGATTTTGGAATTGG
  AGAATATATTAATCAGAAGAAACGTGAGAGATCTGAAGCAGATAAAGAAAAAAGTCACAAAGATGACAGT
  GAATTAGACTTTTCAGCTCTTTGTCCTAAGATTAGCCTCACGGTTGCTGCCAAAGAGTTATCTGTGTCTG
  ACACAGACGTCTCAGAGGTATCCTGGACTGATAATGGGACCTTCAACCTTTCAGAAGGATACACTCCACA
  GACAGACACTTCTGATGATCTTGACCGACCCAGTGAGGAAGTTTTCTCTAGAGATCTTTCAGATTTTCCA
  TCTCTAGAAAATGGCATGGGAACAAATGATGAAGATGAATTAAGCCTTGGTTTGCCCACTGAGCTCAAGA
  GAAAGAAGGAACAGTTGGACAGTGGTCACAGACCAAGCAAAGAGACGCAATCAGCAGCTGGTCTCACCCT
  TCCTCTGAACAGTGACCAAACCTTTCACCTGATGAGCAACCTGGCTGGGGATGTTATCACAGCTGCAGTG
  ACTGCAGCTATCAAAGACCAGTTAGAGGGTGTGCAGCAAGCACTTTCTCAGGCTGCCCCCATCCCAGAAG
  AGGACACAGACACTGAAGAAGGTGATGACTTTGAACTACTTGACCAGTCAGAGCTGGATCAAATTGAGAG
  TGAATTGGGACTTACACAAGACCAGGAAGCAGAAGCACAGCAAAATAAGAAGTCTTCAGGTTTCCTTTCA
  AATCTGCTGGGAGGCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209916 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPEGEDFGPGKSWEVINSKPDERPRLSHCIAESWMNFSIFLQEMSLFKQQSPGKFCLLVCSVCTFFTILG
  SYIPGVILSYLLLLCAFLCPLFKCNDIGQKIYSKIKSVLLKLDFGIGEYINQKKRERSEADKEKSHKDDS
  ELDFSALCPKISLTVAAKELSVSDTDVSEVSWTDNGTFNLSEGYTPQTDTSDDLDRPSEEVFSRDLSDFP
  SLENGMGTNDEDELSLGLPTELKRKKEQLDSGHRPSKETQSAAGLTLPLNSDQTFHLMSNLAGDVITAAV
  TAAIKDQLEGVQQALSQAAPIPEEDTDTEEGDDFELLDQSELDQIESELGLTQDQEAEAQQNKKSSGFLS
  NLLGGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019000
• ORF Size: 1068 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019000.4
• RefSeq Size: 3184 bp
• RefSeq ORF: 1071 bp
• Locus ID: 54463
• UniProt ID: Q9H6L5
• Cytogenetics: 5p15.1
• Protein Families: Transmembrane
• MW: 39.3 kDa
• Gene Summary: The protein encoded by this gene is a cis-Golgi transmembrane protein that may be necessary for the long-term survival of nociceptive and autonomic ganglion neurons. Mutations in this gene are a cause of hereditary sensory and autonomic neuropathy type IIB (HSAN IIB), and this gene may also play a role in susceptibility to vascular dementia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]