RETREG1 (NM_019000) Human Tagged ORF Clone

CAT#: RC209916

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FAM134B (Myc-DDK-tagged)-Human family with sequence similarity 134, member B (FAM134B), transcript variant 2



Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RETREG1
Synonyms FAM134B; JK-1; JK1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209916 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGAAGGTGAAGACTTTGGACCAGGCAAAAGCTGGGAAGTTATCAATTCCAAACCAGATGAAAGAC
CCAGGCTCAGCCACTGTATTGCAGAATCATGGATGAATTTCAGCATATTTCTTCAAGAAATGTCTCTTTT
TAAACAGCAGAGCCCTGGCAAGTTTTGTCTCCTGGTCTGTAGTGTGTGCACATTTTTTACGATCTTGGGA
AGTTACATTCCTGGGGTTATACTCAGCTATCTACTGTTACTGTGTGCATTTTTGTGTCCATTGTTTAAAT
GTAATGATATTGGACAAAAAATTTACAGCAAAATTAAGTCAGTTCTGCTGAAACTGGATTTTGGAATTGG
AGAATATATTAATCAGAAGAAACGTGAGAGATCTGAAGCAGATAAAGAAAAAAGTCACAAAGATGACAGT
GAATTAGACTTTTCAGCTCTTTGTCCTAAGATTAGCCTCACGGTTGCTGCCAAAGAGTTATCTGTGTCTG
ACACAGACGTCTCAGAGGTATCCTGGACTGATAATGGGACCTTCAACCTTTCAGAAGGATACACTCCACA
GACAGACACTTCTGATGATCTTGACCGACCCAGTGAGGAAGTTTTCTCTAGAGATCTTTCAGATTTTCCA
TCTCTAGAAAATGGCATGGGAACAAATGATGAAGATGAATTAAGCCTTGGTTTGCCCACTGAGCTCAAGA
GAAAGAAGGAACAGTTGGACAGTGGTCACAGACCAAGCAAAGAGACGCAATCAGCAGCTGGTCTCACCCT
TCCTCTGAACAGTGACCAAACCTTTCACCTGATGAGCAACCTGGCTGGGGATGTTATCACAGCTGCAGTG
ACTGCAGCTATCAAAGACCAGTTAGAGGGTGTGCAGCAAGCACTTTCTCAGGCTGCCCCCATCCCAGAAG
AGGACACAGACACTGAAGAAGGTGATGACTTTGAACTACTTGACCAGTCAGAGCTGGATCAAATTGAGAG
TGAATTGGGACTTACACAAGACCAGGAAGCAGAAGCACAGCAAAATAAGAAGTCTTCAGGTTTCCTTTCA
AATCTGCTGGGAGGCCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209916 protein sequence
Red=Cloning site Green=Tags(s)

MPEGEDFGPGKSWEVINSKPDERPRLSHCIAESWMNFSIFLQEMSLFKQQSPGKFCLLVCSVCTFFTILG
SYIPGVILSYLLLLCAFLCPLFKCNDIGQKIYSKIKSVLLKLDFGIGEYINQKKRERSEADKEKSHKDDS
ELDFSALCPKISLTVAAKELSVSDTDVSEVSWTDNGTFNLSEGYTPQTDTSDDLDRPSEEVFSRDLSDFP
SLENGMGTNDEDELSLGLPTELKRKKEQLDSGHRPSKETQSAAGLTLPLNSDQTFHLMSNLAGDVITAAV
TAAIKDQLEGVQQALSQAAPIPEEDTDTEEGDDFELLDQSELDQIESELGLTQDQEAEAQQNKKSSGFLS
NLLGGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019000
ORF Size 1068 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019000.4
RefSeq Size 3184 bp
RefSeq ORF 1071 bp
Locus ID 54463
UniProt ID Q9H6L5
Cytogenetics 5p15.1
Protein Families Transmembrane
MW 39.3 kDa
Gene Summary The protein encoded by this gene is a cis-Golgi transmembrane protein that may be necessary for the long-term survival of nociceptive and autonomic ganglion neurons. Mutations in this gene are a cause of hereditary sensory and autonomic neuropathy type IIB (HSAN IIB), and this gene may also play a role in susceptibility to vascular dementia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
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