PLEKHA7 (NM_175058) Human Tagged ORF Clone

CAT#: RC209861

PLEKHA7 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family A member 7 (PLEKHA7)

Product Images

Circular map for RC209861

• 

  Western blot validation of overexpression lysate (Cat# LY406299) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209861 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLEKHA7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209861 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGACGGTCGGGCGGGACACTTTACCTGAGCATTGGTCCTACGGGGTGTGCCGGGATGGCC
  GCGTCTTCTTCATCAATGACCAGCTCCGCTGCACGACCTGGCTGCATCCGCGCACCGGGGAGCCCGTCAA
  CTCGGGCCACATGATCCGCTCAGACCTGCCCCGCGGCTGGGAGGAGGGCTTCACGGAGGAGGGCGCCAGC
  TACTTCATCGACCATAACCAGCAGACCACAGCATTCAGGCATCCTGTGACGGGACAGTTTTCTCCAGAAA
  ATAGTGAATTCATTCTTCAAGAAGAGCCGAATCCACATATGTCGAAGCAAGACAGAAACCAAAGACCGTC
  CAGCATGGTCAGTGAAACATCCACGGCTGGGACCGCCTCCACCCTGGAGGCCAAGCCTGGACCCAAGATC
  ATAAAGTCCAGCAGTAAAGTCCACAGCTTTGGGAAGAGAGACCAGGCCATTCGGAGGAACCCCAATGTTC
  CCGTGGTGGTGAGGGGCTGGCTGCACAAGCAGGACAGTTCTGGGATGAGGCTGTGGAAAAGGAGGTGGTT
  TGTGCTTGCTGATTACTGCTTATTTTACTATAAAGACAGCCGAGAAGAAGCGGTCCTCGGGAGCATCCCC
  TTGCCCAGCTACGTGATCTCTCCTGTGGCCCCTGAGGATCGCATAAGCCGCAAATATTCCTTTAAGGCTG
  TGCACACGGGGATGCGAGCGCTCATCTATAACAGCTCCACAGCGGGCTCTCAGGCCGAGCAGTCAGGCAT
  GAGGACCTACTACTTCAGTGCCGACACCCAGGAGGACATGAACGCTTGGGTCAGGGCCATGAACCAGGCT
  GCACAGGTGCTGTCTCGATCGTCACTGAAGAGGGATATGGAGAAGGTGGAGCGGCAGGCTGTCCCCCAGG
  CCAACCACACAGAGTCCTGTCACGAATGTGGCCGGGTGGGACCCGGACATACGAGAGATTGTCCTCATCG
  TGGCCATGATGACATTGTCAACTTCGAGAGGCAGGAGCAGGAGGGAGAGCAGTACCGTTCCCAGAGGGAC
  CCACTGGAGGGCAAGCGGGACCGGAGCAAGGCCAGGTCTCCGTACTCGCCAGCCGAGGAGGATGCCTTGT
  TTATGGATTTACCCACTGGCCCAAGAGGCCAGCAGGCACAGCCCCAACGGGCAGAGAAGAATGGAATGCT
  GCCTGCCTCATATGGCCCAGGAGAACAGAATGGGACTGGTGGGTACCAGCGGGCCTTTCCTCCCAGGACC
  AACCCTGAAAAACACAGCCAAAGGAAGAGCAATCTGGCCCAGGTGGAGCACTGGGCAAGGGCCCAGAAAG
  GGGATAGCAGGAGTCTTCCCTTGGACCAGACGCTTCCTCGCCAGGGTCCTGGCCAATCCCTGTCCTTCCC
  AGAAAACTACCAGACTCTTCCCAAGAGCACCCGACACCCCTCGGGGGGCTCCTCGCCACCTCCCCGAAAC
  CTGCCAAGTGACTACAAGTATGCGCAGGACCGAGCCAGCCACCTGAAGATGTCGAGTGAAGAGCGCCGGG
  CGCACCGGGATGGCACCGTGTGGCAGCTCTACGAGTGGCAGCAGCGCCAGCAGTTCCGGCACGGCAGCCC
  CACAGCGCCCATCTGCCTTGGCTCCCCAGAGTTCACCGACCAGGGCCGGAGCAGGAGCATGCTAGAGGTG
  CCCCGCTCCATCTCTGTGCCTCCATCTCCCTCGGACATCCCTCCCCCAGGACCCCCAAGGGTCTTCCCAC
  CCCGGCGGCCACACACACCAGCAGAGCGAGTCACAGTGAAGCCACCGGACCAGAGGAGGAGTGTGGACAT
  CTCGCTGGGGGATTCTCCAAGGAGGGCACGGGGCCACGCTGTCAAGAACTCATCTCATGTGGACCGACGT
  TCCATGCCCTCCATGGGTTACATGACCCACACCGTCAGCGCTCCCAGTTTACATGGAAAATCGGCTGATG
  ATACCTACCTCCAGCTGAAGAAAGACCTGGAGTACCTGGATCTAAAGATGACAGGCCGGGACCTTCTCAA
  GGATCGAAGTCTGAAGCCTGTGAAGATCGCTGAGAGCGACACTGACGTCAAACTGAGCATCTTCTGTGAA
  CAAGACAGGGTCCTCCAGGACTTGGAAGACAAGATACGAGCCCTTAAAGAGAACAAAGACCAGCTAGAAT
  CTGTGCTGGAGGTGTTGCACAGACAGATGGAGCAGTACCGAGACCAGCCCCAGCACTTGGAGAAGATTGC
  CTACCAGCAGAAGTTGCTGCAGGAGGACCTTGTCCATATCCGAGCTGAGCTCTCCAGAGAGTCCACTGAG
  ATGGAAAATGCTTGGAACGAATACCTGAAGTTGGAGAATGATGTGGAACAGCTGAAGCAGACCCTGCAGG
  AGCAACACAGAAGAGCCTTTTTTTTCCAGGAGAAATCGCAGATACAGAAAGATCTATGGAGAATTGAAGA
  TGTCACTGCAGGCCTGAGTGCAAATAAAGAGAACTTCAGAATTCTAGTGGAGTCAGTAAAAAATCCGGAG
  AGAAAAACGGTGCCTTTGTTTCCTCACCCGCCTGTGCCTTCACTCTCAACTTCTGAGAGCAAGCCGCCCC
  CACAGCCCAGTCCTCCCACCAGCCCTGTGCGGACCCCTCTGGAGGTTCGACTCTTCCCCCAGCTGCAAAC
  CTACGTGCCGTACCGACCTCACCCACCCCAGCTGAGGAAAGTGACATCCCCCCTTCAGTCACCAACTAAG
  GCGAAGCCCAAAGTTGAAGATGAAGCACCTCCCAGGCCCCCACTCCCCGAACTCTACAGCCCAGAGGACC
  AGCCCCCGGCTGTGCCGCCTCTGCCAAGAGAGGCCACCATCATCCGGCACACATCTGTGCGGGGCCTCAA
  GCGGCAGTCAGACGAGAGGAAGCGAGACCGGGAGCTGGGGCAGTGTGTGAATGGGGATTCCAGGGTGGAG
  CCGCGGTCGTATGTCAGTGAGCCTGAGCTGGCGACCCTCAGCGGGGACATGGCCCAGCCCTCCCTAGGAC
  TTGTGGGCCCTGAGAGCAGGTACCAGACGCTGCCAGGCAGAGGGCTCTCAGGGTCCACGTCAAGGCTCCA
  GCAGTCGTCCACCATTGCTCCCTACGTCACACTCCGGAGGGGTCTCAATGCCGAAAGCAGCAAGGCGACC
  TTCCCTAGACCTAAGAGTGCCTTGGAGCGCCTGTACTCAGGGGATCACCAGCGAGGCAAGATGAGTGCAG
  AGGAGCAGCTGGAGCGCATGAAGCGACACCAGAAGGCCCTGGTCCGAGAGCGCAAGAGGACACTGGGCCA
  AGGGGAGAGGACGGGCCTGCCCTCATCTCGCTACCTCAGCCGGCCGCTCCCTGGAGATCTTGGCTCAGTA
  TGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209861 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAATVGRDTLPEHWSYGVCRDGRVFFINDQLRCTTWLHPRTGEPVNSGHMIRSDLPRGWEEGFTEEGAS
  YFIDHNQQTTAFRHPVTGQFSPENSEFILQEEPNPHMSKQDRNQRPSSMVSETSTAGTASTLEAKPGPKI
  IKSSSKVHSFGKRDQAIRRNPNVPVVVRGWLHKQDSSGMRLWKRRWFVLADYCLFYYKDSREEAVLGSIP
  LPSYVISPVAPEDRISRKYSFKAVHTGMRALIYNSSTAGSQAEQSGMRTYYFSADTQEDMNAWVRAMNQA
  AQVLSRSSLKRDMEKVERQAVPQANHTESCHECGRVGPGHTRDCPHRGHDDIVNFERQEQEGEQYRSQRD
  PLEGKRDRSKARSPYSPAEEDALFMDLPTGPRGQQAQPQRAEKNGMLPASYGPGEQNGTGGYQRAFPPRT
  NPEKHSQRKSNLAQVEHWARAQKGDSRSLPLDQTLPRQGPGQSLSFPENYQTLPKSTRHPSGGSSPPPRN
  LPSDYKYAQDRASHLKMSSEERRAHRDGTVWQLYEWQQRQQFRHGSPTAPICLGSPEFTDQGRSRSMLEV
  PRSISVPPSPSDIPPPGPPRVFPPRRPHTPAERVTVKPPDQRRSVDISLGDSPRRARGHAVKNSSHVDRR
  SMPSMGYMTHTVSAPSLHGKSADDTYLQLKKDLEYLDLKMTGRDLLKDRSLKPVKIAESDTDVKLSIFCE
  QDRVLQDLEDKIRALKENKDQLESVLEVLHRQMEQYRDQPQHLEKIAYQQKLLQEDLVHIRAELSRESTE
  MENAWNEYLKLENDVEQLKQTLQEQHRRAFFFQEKSQIQKDLWRIEDVTAGLSANKENFRILVESVKNPE
  RKTVPLFPHPPVPSLSTSESKPPPQPSPPTSPVRTPLEVRLFPQLQTYVPYRPHPPQLRKVTSPLQSPTK
  AKPKVEDEAPPRPPLPELYSPEDQPPAVPPLPREATIIRHTSVRGLKRQSDERKRDRELGQCVNGDSRVE
  PRSYVSEPELATLSGDMAQPSLGLVGPESRYQTLPGRGLSGSTSRLQQSSTIAPYVTLRRGLNAESSKAT
  FPRPKSALERLYSGDHQRGKMSAEEQLERMKRHQKALVRERKRTLGQGERTGLPSSRYLSRPLPGDLGSV
  C
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175058
• ORF Size: 3363 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_175058.3, NP_778228.2
• RefSeq Size: 4820 bp
• RefSeq ORF: 3366 bp
• Locus ID: 144100
• UniProt ID: Q6IQ23
• Cytogenetics: 11p15.2-p15.1
• MW: 127.1 kDa
• Gene Summary: Required for zonula adherens biogenesis and maintenance. Acts via its interaction with KIAA1543/Nezha, which anchors microtubules at their minus-ends to zonula adherens, leading to the recruitment of KIFC3 kinesin to the junctional site.[UniProtKB/Swiss-Prot Function]