PLEKHA7 (NM_175058) Human Tagged ORF Clone

CAT#: RC209861

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PLEKHA7 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family A member 7 (PLEKHA7)



  "NM_175058" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 680.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PLEKHA7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209861 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCGACGGTCGGGCGGGACACTTTACCTGAGCATTGGTCCTACGGGGTGTGCCGGGATGGCC
GCGTCTTCTTCATCAATGACCAGCTCCGCTGCACGACCTGGCTGCATCCGCGCACCGGGGAGCCCGTCAA
CTCGGGCCACATGATCCGCTCAGACCTGCCCCGCGGCTGGGAGGAGGGCTTCACGGAGGAGGGCGCCAGC
TACTTCATCGACCATAACCAGCAGACCACAGCATTCAGGCATCCTGTGACGGGACAGTTTTCTCCAGAAA
ATAGTGAATTCATTCTTCAAGAAGAGCCGAATCCACATATGTCGAAGCAAGACAGAAACCAAAGACCGTC
CAGCATGGTCAGTGAAACATCCACGGCTGGGACCGCCTCCACCCTGGAGGCCAAGCCTGGACCCAAGATC
ATAAAGTCCAGCAGTAAAGTCCACAGCTTTGGGAAGAGAGACCAGGCCATTCGGAGGAACCCCAATGTTC
CCGTGGTGGTGAGGGGCTGGCTGCACAAGCAGGACAGTTCTGGGATGAGGCTGTGGAAAAGGAGGTGGTT
TGTGCTTGCTGATTACTGCTTATTTTACTATAAAGACAGCCGAGAAGAAGCGGTCCTCGGGAGCATCCCC
TTGCCCAGCTACGTGATCTCTCCTGTGGCCCCTGAGGATCGCATAAGCCGCAAATATTCCTTTAAGGCTG
TGCACACGGGGATGCGAGCGCTCATCTATAACAGCTCCACAGCGGGCTCTCAGGCCGAGCAGTCAGGCAT
GAGGACCTACTACTTCAGTGCCGACACCCAGGAGGACATGAACGCTTGGGTCAGGGCCATGAACCAGGCT
GCACAGGTGCTGTCTCGATCGTCACTGAAGAGGGATATGGAGAAGGTGGAGCGGCAGGCTGTCCCCCAGG
CCAACCACACAGAGTCCTGTCACGAATGTGGCCGGGTGGGACCCGGACATACGAGAGATTGTCCTCATCG
TGGCCATGATGACATTGTCAACTTCGAGAGGCAGGAGCAGGAGGGAGAGCAGTACCGTTCCCAGAGGGAC
CCACTGGAGGGCAAGCGGGACCGGAGCAAGGCCAGGTCTCCGTACTCGCCAGCCGAGGAGGATGCCTTGT
TTATGGATTTACCCACTGGCCCAAGAGGCCAGCAGGCACAGCCCCAACGGGCAGAGAAGAATGGAATGCT
GCCTGCCTCATATGGCCCAGGAGAACAGAATGGGACTGGTGGGTACCAGCGGGCCTTTCCTCCCAGGACC
AACCCTGAAAAACACAGCCAAAGGAAGAGCAATCTGGCCCAGGTGGAGCACTGGGCAAGGGCCCAGAAAG
GGGATAGCAGGAGTCTTCCCTTGGACCAGACGCTTCCTCGCCAGGGTCCTGGCCAATCCCTGTCCTTCCC
AGAAAACTACCAGACTCTTCCCAAGAGCACCCGACACCCCTCGGGGGGCTCCTCGCCACCTCCCCGAAAC
CTGCCAAGTGACTACAAGTATGCGCAGGACCGAGCCAGCCACCTGAAGATGTCGAGTGAAGAGCGCCGGG
CGCACCGGGATGGCACCGTGTGGCAGCTCTACGAGTGGCAGCAGCGCCAGCAGTTCCGGCACGGCAGCCC
CACAGCGCCCATCTGCCTTGGCTCCCCAGAGTTCACCGACCAGGGCCGGAGCAGGAGCATGCTAGAGGTG
CCCCGCTCCATCTCTGTGCCTCCATCTCCCTCGGACATCCCTCCCCCAGGACCCCCAAGGGTCTTCCCAC
CCCGGCGGCCACACACACCAGCAGAGCGAGTCACAGTGAAGCCACCGGACCAGAGGAGGAGTGTGGACAT
CTCGCTGGGGGATTCTCCAAGGAGGGCACGGGGCCACGCTGTCAAGAACTCATCTCATGTGGACCGACGT
TCCATGCCCTCCATGGGTTACATGACCCACACCGTCAGCGCTCCCAGTTTACATGGAAAATCGGCTGATG
ATACCTACCTCCAGCTGAAGAAAGACCTGGAGTACCTGGATCTAAAGATGACAGGCCGGGACCTTCTCAA
GGATCGAAGTCTGAAGCCTGTGAAGATCGCTGAGAGCGACACTGACGTCAAACTGAGCATCTTCTGTGAA
CAAGACAGGGTCCTCCAGGACTTGGAAGACAAGATACGAGCCCTTAAAGAGAACAAAGACCAGCTAGAAT
CTGTGCTGGAGGTGTTGCACAGACAGATGGAGCAGTACCGAGACCAGCCCCAGCACTTGGAGAAGATTGC
CTACCAGCAGAAGTTGCTGCAGGAGGACCTTGTCCATATCCGAGCTGAGCTCTCCAGAGAGTCCACTGAG
ATGGAAAATGCTTGGAACGAATACCTGAAGTTGGAGAATGATGTGGAACAGCTGAAGCAGACCCTGCAGG
AGCAACACAGAAGAGCCTTTTTTTTCCAGGAGAAATCGCAGATACAGAAAGATCTATGGAGAATTGAAGA
TGTCACTGCAGGCCTGAGTGCAAATAAAGAGAACTTCAGAATTCTAGTGGAGTCAGTAAAAAATCCGGAG
AGAAAAACGGTGCCTTTGTTTCCTCACCCGCCTGTGCCTTCACTCTCAACTTCTGAGAGCAAGCCGCCCC
CACAGCCCAGTCCTCCCACCAGCCCTGTGCGGACCCCTCTGGAGGTTCGACTCTTCCCCCAGCTGCAAAC
CTACGTGCCGTACCGACCTCACCCACCCCAGCTGAGGAAAGTGACATCCCCCCTTCAGTCACCAACTAAG
GCGAAGCCCAAAGTTGAAGATGAAGCACCTCCCAGGCCCCCACTCCCCGAACTCTACAGCCCAGAGGACC
AGCCCCCGGCTGTGCCGCCTCTGCCAAGAGAGGCCACCATCATCCGGCACACATCTGTGCGGGGCCTCAA
GCGGCAGTCAGACGAGAGGAAGCGAGACCGGGAGCTGGGGCAGTGTGTGAATGGGGATTCCAGGGTGGAG
CCGCGGTCGTATGTCAGTGAGCCTGAGCTGGCGACCCTCAGCGGGGACATGGCCCAGCCCTCCCTAGGAC
TTGTGGGCCCTGAGAGCAGGTACCAGACGCTGCCAGGCAGAGGGCTCTCAGGGTCCACGTCAAGGCTCCA
GCAGTCGTCCACCATTGCTCCCTACGTCACACTCCGGAGGGGTCTCAATGCCGAAAGCAGCAAGGCGACC
TTCCCTAGACCTAAGAGTGCCTTGGAGCGCCTGTACTCAGGGGATCACCAGCGAGGCAAGATGAGTGCAG
AGGAGCAGCTGGAGCGCATGAAGCGACACCAGAAGGCCCTGGTCCGAGAGCGCAAGAGGACACTGGGCCA
AGGGGAGAGGACGGGCCTGCCCTCATCTCGCTACCTCAGCCGGCCGCTCCCTGGAGATCTTGGCTCAGTA
TGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209861 protein sequence
Red=Cloning site Green=Tags(s)

MAAATVGRDTLPEHWSYGVCRDGRVFFINDQLRCTTWLHPRTGEPVNSGHMIRSDLPRGWEEGFTEEGAS
YFIDHNQQTTAFRHPVTGQFSPENSEFILQEEPNPHMSKQDRNQRPSSMVSETSTAGTASTLEAKPGPKI
IKSSSKVHSFGKRDQAIRRNPNVPVVVRGWLHKQDSSGMRLWKRRWFVLADYCLFYYKDSREEAVLGSIP
LPSYVISPVAPEDRISRKYSFKAVHTGMRALIYNSSTAGSQAEQSGMRTYYFSADTQEDMNAWVRAMNQA
AQVLSRSSLKRDMEKVERQAVPQANHTESCHECGRVGPGHTRDCPHRGHDDIVNFERQEQEGEQYRSQRD
PLEGKRDRSKARSPYSPAEEDALFMDLPTGPRGQQAQPQRAEKNGMLPASYGPGEQNGTGGYQRAFPPRT
NPEKHSQRKSNLAQVEHWARAQKGDSRSLPLDQTLPRQGPGQSLSFPENYQTLPKSTRHPSGGSSPPPRN
LPSDYKYAQDRASHLKMSSEERRAHRDGTVWQLYEWQQRQQFRHGSPTAPICLGSPEFTDQGRSRSMLEV
PRSISVPPSPSDIPPPGPPRVFPPRRPHTPAERVTVKPPDQRRSVDISLGDSPRRARGHAVKNSSHVDRR
SMPSMGYMTHTVSAPSLHGKSADDTYLQLKKDLEYLDLKMTGRDLLKDRSLKPVKIAESDTDVKLSIFCE
QDRVLQDLEDKIRALKENKDQLESVLEVLHRQMEQYRDQPQHLEKIAYQQKLLQEDLVHIRAELSRESTE
MENAWNEYLKLENDVEQLKQTLQEQHRRAFFFQEKSQIQKDLWRIEDVTAGLSANKENFRILVESVKNPE
RKTVPLFPHPPVPSLSTSESKPPPQPSPPTSPVRTPLEVRLFPQLQTYVPYRPHPPQLRKVTSPLQSPTK
AKPKVEDEAPPRPPLPELYSPEDQPPAVPPLPREATIIRHTSVRGLKRQSDERKRDRELGQCVNGDSRVE
PRSYVSEPELATLSGDMAQPSLGLVGPESRYQTLPGRGLSGSTSRLQQSSTIAPYVTLRRGLNAESSKAT
FPRPKSALERLYSGDHQRGKMSAEEQLERMKRHQKALVRERKRTLGQGERTGLPSSRYLSRPLPGDLGSV
C

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_175058
ORF Size 3363 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_175058.3, NP_778228.2
RefSeq Size 4820 bp
RefSeq ORF 3366 bp
Locus ID 144100
UniProt ID Q6IQ23
Cytogenetics 11p15.2-p15.1
MW 127.1 kDa
Gene Summary Required for zonula adherens biogenesis and maintenance. Acts via its interaction with KIAA1543/Nezha, which anchors microtubules at their minus-ends to zonula adherens, leading to the recruitment of KIFC3 kinesin to the junctional site.[UniProtKB/Swiss-Prot Function]

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