HIBCH (NM_014362) Human Tagged ORF Clone

CAT#: RC209814

HIBCH (Myc-DDK-tagged)-Human 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RC209814

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HIBCH (Cat# RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH(Cat# TA501317). Positive lysates LY402324 (100ug) and LC402324 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402324) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209814 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HIBCH protein (Cat# TP309814). The protein was produced from HEK293T cells transfected with HIBCH cDNA clone (Cat# RC209814) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HIBCH
• Synonyms: HIBYLCOAH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209814 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAGCGCGAGATGTGGAGGCTCATGTCGAGGTTTAATGCATTCAAAAGGACTAATACCATACTGC
  ACCATTTGAGAATGTCCAAGCACACAGATGCAGCAGAAGAGGTGCTATTGGGAAAAAAAGGTTGCACGGG
  AGTCATAACACTAAACAGACCAAAGTTCCTCAATGCACTGACTCTTAATATGATTCGGCAGATTTATCCA
  CAGCTAAAGAAGTGGGAACAAGATCCTGAAACTTTCCTGATCATTATAAAGGGAGCAGGAGGAAAGGCTT
  TCTGTGCCGGGGGTGATATCAGAGTGATCTCGGAAGCTGAAAAGGCAAAACAGAAGATAGCTCCAGTTTT
  CTTCAGAGAAGAATATATGCTGAATAATGCTGTTGGTTCTTGCCAGAAACCTTATGTTGCACTTATTCAT
  GGAATTACAATGGGTGGGGGAGTTGGTCTCTCAGTCCATGGGCAATTTCGAGTGGCTACAGAAAAGTGTC
  TTTTTGCTATGCCAGAAACTGCAATAGGACTGTTCCCTGATGTGGGTGGAGGTTATTTCTTGCCACGACT
  CCAAGGAAAACTTGGTTACTTCCTTGCATTAACAGGATTCAGACTAAAAGGAAGAGATGTGTACAGAGCA
  GGAATTGCTACACACTTTGTAGATTCTGAAAAGTTGGCCATGTTAGAGGAAGATTTGTTAGCCTTGAAAT
  CTCCTTCAAAAGAAAATATTGCATCTGTCTTAGAAAATTACCATACAGAGTCTAAGATTGATCGAGACAA
  GTCTTTTATACTTGAGGAACACATGGACAAAATAAACAGTTGTTTTTCAGCCAATACTGTGGAAGAAATT
  ATTGAAAACTTACAGCAAGATGGTTCATCTTTTGCCCTAGAGCAATTGAAGGTAATTAATAAAATGTCTC
  CAACATCTCTAAAGATCACACTAAGGCAACTCATGGAGGGGTCTTCAAAGACCTTGCAAGAAGTACTAAC
  TATGGAGTATCGGCTAAGTCAAGCTTGTATGAGAGGTCATGACTTTCATGAAGGCGTTAGAGCTGTTTTA
  ATTGATAAAGACCAGAGTCCAAAATGGAAACCAGCTGATCTAAAAGAAGTTACTGAGGAAGATTTGAATA
  ATCACTTTAAGTCTTTGGGAAGCAGTGATTTGAAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209814 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLGKKGCTGVITLNRPKFLNALTLNMIRQIYP
  QLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIH
  GITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRA
  GIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEI
  IENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVL
  IDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014362
• ORF Size: 1158 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014362.4
• RefSeq Size: 1958 bp
• RefSeq ORF: 1161 bp
• Locus ID: 26275
• UniProt ID: Q6NVY1
• Cytogenetics: 2q32.2
• Domains: ECH
• Protein Pathways: beta-Alanine metabolism, Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• MW: 43.4 kDa
• Gene Summary: This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]