TMEM35 (TMEM35A) (NM_021637) Human Tagged ORF Clone

CAT#: RC209790

TMEM35 (Myc-DDK-tagged)-Human transmembrane protein 35 (TMEM35)

Product Images

Western blot validation of overexpression lysate (Cat# LY411954) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209790 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TMEM35A protein (Cat# TP309790). The protein was produced from HEK293T cells transfected with TMEM35A cDNA clone (Cat# RC209790) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM35
• Synonyms: NACHO; TMEM35; TUF-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209790 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCCCCCAGAACCGTAACTATTGTGGCCCTCTCAGTGGCCCTGGGACTCTTCTTTGTTTTCATGG
  GGACTATCAAGCTGACCCCCAGGCTCAGCAAGGATGCCTACAGTGAGATGAAACGTGCTTACAAGAGCTA
  TGTTCGAGCCCTCCCTCTGCTGAAGAAAATGGGGATCAATTCCATTCTCCTCCGAAAAAGCATTGGTGCC
  CTTGAAGTGGCCTGTGGCATCGTCATGACCCTTGTGCCTGGGCGTCCCAAAGATGTGGCCAACTTCTTCC
  TACTGTTGCTGGTGTTGGCTGTGCTCTTCTTCCACCAGCTGGTCGGTGATCCTCTCAAACGCTACGCCCA
  TGCTCTGGTGTTTGGAATCCTGCTCACTTGCCGCCTGCTGATTGCTCGCAAGCCCGAAGACCGGTCTTCT
  GAGAAGAAGCCTTTGCCAGGGAATGCTGAGGAGCAACCCTCCTTATATGAGAAGGCCCCTCAGGGCAAAG
  TGAAGGTGTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209790 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASPRTVTIVALSVALGLFFVFMGTIKLTPRLSKDAYSEMKRAYKSYVRALPLLKKMGINSILLRKSIGA
  LEVACGIVMTLVPGRPKDVANFFLLLLVLAVLFFHQLVGDPLKRYAHALVFGILLTCRLLIARKPEDRSS
  EKKPLPGNAEEQPSLYEKAPQGKVKVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021637
• ORF Size: 501 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021637.3
• RefSeq Size: 2086 bp
• RefSeq ORF: 504 bp
• Locus ID: 59353
• UniProt ID: Q53FP2
• Cytogenetics: Xq22.1
• Protein Families: Transmembrane
• MW: 18.4 kDa
• Gene Summary: A soluble peptide released by shedding may interact with NGFR and modulate neurite outgrowth.[UniProtKB/Swiss-Prot Function]