ADNP (NM_015339) Human Tagged ORF Clone

CAT#: RC209788

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ADNP (Myc-DDK-tagged)-Human activity-dependent neuroprotector homeobox (ADNP), transcript variant 1



  "NM_015339" in other vectors (6)

USD 600.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Product Name ADNP (NM_015339) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ADNP
Synonyms ADNP1; HVDAS; MRD28
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC209788 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCAACTTCCTGTCAACAATCTTGGCAGTTTAAGAAAAGCCCGGAAAACTGTGAAAAAAATACTTA
GTGACATTGGGTTGGAATACTGTAAAGAACATATAGAAGATTTTAAACAATTTGAACCTAATGACTTTTA
TTTGAAAAACACTACATGGGAGGATGTAGGACTGTGGGACCCATCACTTACGAAAAACCAGGACTATCGG
ACAAAACCTTTCTGCTGCAGCGCTTGTCCATGTTCCTCAAAATTCTTCTCTGCCTACAAAAGTCATTTCC
GCAATGTCCATAGTGAAGACTTTGAAAATAGGATTCTCCTTAATTGCCCCTACTGTACCTTCAATGCAGA
CAAAAAGACTTTGGAAACACACATTAAAATATTTCATGCTCCGAACGCCAGCGCACCAAGTAGCAGCCTC
AGCACTTTCAAAGATAAAAACAAAAATGATGGCCTTAAACCTAAGCAGGCTGACAGTGTAGAGCAAGCTG
TTTATTACTGTAAGAAGTGCACTTACCGAGATCCTCTTTATGAAATAGTTAGGAAGCACATTTACAGGGA
ACATTTTCAGCATGTGGCAGCACCTTACATAGCAAAGGCAGGAGAAAAATCACTCAATGGGGCAGTCCCC
TTAGGCTCGAATGCCCGAGAAGAGAGTAGTATTCACTGCAAACGATGCCTTTTCATGCCAAAGTCCTATG
AAGCTTTGGTACAGCATGTCATCGAAGACCATGAACGTATAGGCTATCAGGTCACTGCCATGATTGGGCA
CACAAATGTAGTGGTTCCCCGATCCAAACCCTTGATGCTAATTGCTCCCAAACCTCAAGACAAGAAGAGC
ATGGGACTCCCACCAAGGATCGGTTCCCTTGCTTCTGGAAATGTCCGGTCTTTACCATCACAGCAGATGG
TGAATCGACTCTCAATACCAAAGCCTAACTTAAATTCTACAGGAGTCAACATGATGTCCAGTGTTCATCT
GCAGCAGAACAACTATGGAGTCAAATCTGTAGGCCAGGGTTACAGTGTTGGTCAGTCAATGAGACTGGGT
CTAGGTGGCAACGCACCAGTTTCCATTCCTCAACAATCTCAGTCTGTAAAGCAGTTACTTCCAAGTGGAA
ACGGAAGGTCTTATGGGCTTGGGTCAGAGCAGAGGTCCCAGGCACCAGCAAGATACTCCCTGCAGTCTGC
TAATGCCTCTTCTCTCTCATCGGGCCAGTTAAAGTCTCCTTCCCTCTCTCAGTCACAGGCATCCAGAGTG
TTAGGTCAGTCCAGTTCCAAACCTGCTGCAGCTGCCACAGGCCCTCCCCCAGGTAACACTTCCTCAACTC
AAAAGTGGAAAATATGTACAATCTGTAATGAGCTTTTTCCTGAAAATGTCTATAGTGTGCACTTCGAAAA
AGGACATAAAGCTGAGAAAGTCCCAGCAGTAGCCAACTACATTATGAAAATACACAATTTTACTAGCAAA
TGCCTCTACTGTAATCGCTATTTACCCACAGATACTCTGCTCAACCATATGTTAATTCATGGTCTGTCTT
GTCCATATTGCCGTTCAACTTTCAATGATGTGGAAAAGATGGCCGCACACATGCGGATGGTTCACATTGA
TGAAGAGATGGGACCTAAAACAGATTCTACTTTGAGTTTTGATTTGACATTGCAGCAGGGTAGTCACACT
AACATCCATCTCCTGGTAACTACATACAATCTGAGGGATGCCCCAGCTGAATCTGTTGCTTACCATGCCC
AAAATAATCCTCCAGTTCCTCCAAAGCCACAGCCAAAGGTTCAGGAAAAGGCAGATATCCCTGTAAAAAG
TTCACCTCAAGCTGCAGTGCCCTATAAAAAAGATGTTGGGAAAACCCTTTGTCCTCTTTGCTTTTCAATC
CTAAAAGGACCCATATCTGATGCACTTGCACATCACTTACGAGAGAGGCACCAAGTTATTCAGACGGTTC
ATCCAGTTGAGAAAAAGCTCACCTACAAATGTATCCATTGCCTTGGTGTGTATACCAGCAACATGACCGC
CTCAACTATCACTCTGCATCTAGTTCACTGCAGGGGCGTTGGAAAGACCCAAAATGGCCAGGATAAGACA
AATGCACCCTCTCGGCTTAATCAGTCTCCAAGTCTGGCACCTGTGAAGCGCACTTACGAGCAAATGGAAT
TTCCCTTACTGAAAAAACGAAAGTTAGATGATGATAGTGATTCACCCAGCTTCTTTGAAGAGAAGCCTGA
AGAGCCTGTTGTTTTAGCTTTAGACCCCAAGGGTCATGAAGATGATTCCTATGAAGCCAGGAAAAGCTTT
CTAACAAAGTATTTCAACAAACAGCCCTATCCCACCAGGAGAGAAATTGAGAAGCTAGCAGCCAGTTTAT
GGTTATGGAAGAGTGACATCGCTTCCCATTTTAGTAACAAAAGGAAGAAGTGTGTCCGTGATTGTGAAAA
GTACAAGCCTGGCGTGTTGCTGGGGTTTAACATGAAAGAATTAAATAAAGTCAAGCATGAGATGGATTTT
GACGCTGAGTGGCTATTTGAAAATCATGATGAGAAGGATTCCAGAGTCAATGCTAGTAAGACTGCTGACA
AAAAGCTCAACCTTGGGAAGGAAGATGACAGTTCCTCAGACAGTTTTGAAAATTTGGAAGAAGAATCCAA
TGAAAGTGGTAGCCCTTTTGACCCTGTTTTTGAAGTTGAACCTAAAATCTCTAACGATAACCCAGAGGAA
CATGTACTGAAGGTAATTCCTGAGGATGCTTCAGAATCTGAGGAGAAGCTAGACCAAAAAGAGGATGGTT
CAAAATACGAAACTATTCATTTGACTGAGGAACCAACCAAACTAATGCACAATGCATCTGATAGTGAGGT
TGACCAAGACGATGTTGTTGAGTGGAAAGACGGTGCTTCTCCATCTGAGAGTGGGCCTGGATCCCAACAA
GTGTCAGACTTTGAGGACAATACCTGCGAAATGAAACCAGGAACCTGGTCTGACGAGTCTTCCCAAAGCG
AAGATGCAAGGAGCAGTAAGCCAGCTGCCAAAAAAAAGGCTACCATGCAAGGTGACAGAGAGCAGTTGAA
ATGGAAGAATAGTTCCTATGGAAAAGTTGAAGGGTTTTGGTCTAAGGACCAGTCACAGTGGAAGAATGCA
TCTGAGAATGATGAGCGCTTATCTAACCCCCAGATTGAGTGGCAGAATAGCACAATTGACAGTGAGGATG
GGGAACAGTTTGACAACATGACTGATGGAGTAGCTGAGCCCATGCATGGCAGCTTAGCCGGAGTTAAACT
GAGCAGCCAACAGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209788 protein sequence
Red=Cloning site Green=Tags(s)

MFQLPVNNLGSLRKARKTVKKILSDIGLEYCKEHIEDFKQFEPNDFYLKNTTWEDVGLWDPSLTKNQDYR
TKPFCCSACPCSSKFFSAYKSHFRNVHSEDFENRILLNCPYCTFNADKKTLETHIKIFHAPNASAPSSSL
STFKDKNKNDGLKPKQADSVEQAVYYCKKCTYRDPLYEIVRKHIYREHFQHVAAPYIAKAGEKSLNGAVP
LGSNAREESSIHCKRCLFMPKSYEALVQHVIEDHERIGYQVTAMIGHTNVVVPRSKPLMLIAPKPQDKKS
MGLPPRIGSLASGNVRSLPSQQMVNRLSIPKPNLNSTGVNMMSSVHLQQNNYGVKSVGQGYSVGQSMRLG
LGGNAPVSIPQQSQSVKQLLPSGNGRSYGLGSEQRSQAPARYSLQSANASSLSSGQLKSPSLSQSQASRV
LGQSSSKPAAAATGPPPGNTSSTQKWKICTICNELFPENVYSVHFEKGHKAEKVPAVANYIMKIHNFTSK
CLYCNRYLPTDTLLNHMLIHGLSCPYCRSTFNDVEKMAAHMRMVHIDEEMGPKTDSTLSFDLTLQQGSHT
NIHLLVTTYNLRDAPAESVAYHAQNNPPVPPKPQPKVQEKADIPVKSSPQAAVPYKKDVGKTLCPLCFSI
LKGPISDALAHHLRERHQVIQTVHPVEKKLTYKCIHCLGVYTSNMTASTITLHLVHCRGVGKTQNGQDKT
NAPSRLNQSPSLAPVKRTYEQMEFPLLKKRKLDDDSDSPSFFEEKPEEPVVLALDPKGHEDDSYEARKSF
LTKYFNKQPYPTRREIEKLAASLWLWKSDIASHFSNKRKKCVRDCEKYKPGVLLGFNMKELNKVKHEMDF
DAEWLFENHDEKDSRVNASKTADKKLNLGKEDDSSSDSFENLEEESNESGSPFDPVFEVEPKISNDNPEE
HVLKVIPEDASESEEKLDQKEDGSKYETIHLTEEPTKLMHNASDSEVDQDDVVEWKDGASPSESGPGSQQ
VSDFEDNTCEMKPGTWSDESSQSEDARSSKPAAKKKATMQGDREQLKWKNSSYGKVEGFWSKDQSQWKNA
SENDERLSNPQIEWQNSTIDSEDGEQFDNMTDGVAEPMHGSLAGVKLSSQQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_015339
ORF Size 3306 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015339.1, NM_015339.2, NM_015339.3, NM_015339.4, NP_056154.1
RefSeq Size 6158 bp
RefSeq ORF 3309 bp
Locus ID 23394
Cytogenetics 20q13.13
Domains homeobox, zf-C2H2
Protein Families Transcription Factors
MW 123.4 kDa
Gene Summary Vasoactive intestinal peptide is a neuroprotective factor that has a stimulatory effect on the growth of some tumor cells and an inhibitory effect on others. This gene encodes a protein that is upregulated by vasoactive intestinal peptide and may be involved in its stimulatory effect on certain tumor cells. The encoded protein contains one homeobox and nine zinc finger domains, suggesting that it functions as a transcription factor. This gene is also upregulated in normal proliferative tissues. Finally, the encoded protein may increase the viability of certain cell types through modulation of p53 activity. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

Citations (1)

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