Hormone sensitive lipase (LIPE) (NM_005357) Human Tagged ORF Clone

CAT#: RC209729

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LIPE (Myc-DDK-tagged)-Human lipase, hormone-sensitive (LIPE)



Reconstitution Protocol

USD 937.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol LIPE
Synonyms AOMS4; FPLD6; HSL; LHS; REH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209729 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCAGGTTCTAAGTCAGTGTCTAGGTCAGACTGGCAACCTGAACCACACCAGAGGCCTATAACCC
CGCTAGAGCCTGGGCCAGAAAAGACACCCATAGCCCAGCCAGAATCGAAGACTCTGCAGGGATCCAATAC
CCAACAGAAGCCTGCTTCAAACCAAAGACCCCTCACCCAGCAGGAGACCCCTGCACAACATGATGCTGAA
TCCCAGAAGGAACCTAGAGCCCAACAAAAATCTGCTTCACAAGAGGAATTTCTTGCCCCACAGAAGCCCG
CACCACAGCAATCACCTTACATCCAAAGGGTGCTGCTCACTCAACAGGAAGCTGCCTCCCAGCAGGGACC
TGGGCTAGGAAAAGAATCTATAACTCAACAGGAGCCAGCATTGAGACAAAGACATGTAGCCCAGCCAGGG
CCTGGGCCAGGAGAGCCACCTCCAGCTCAACAAGAAGCTGAATCAACACCTGCGGCCCAGGCTAAACCTG
GAGCCAAAAGGGAGCCATCTGCCCCGACTGAATCTACGTCCCAAGAGACACCTGAACAGTCAGACAAGCA
AACAACGCCAGTCCAGGGAGCCAAATCCAAGCAGGGATCTTTGACAGAGCTGGGATTTCTAACAAAACTT
CAGGAACTATCCATACAGCGATCAGCCCTAGAGTGGAAGGCACTTTCTGAGTGGGTCACAGATTCTGAGT
CAGAATCAGATGTGGGATCATCTTCAGACACAGATTCTCCAGCCACGATGGGTGGAATGGTGGCCCAGGG
AGTGAAGCTAGGCTTCAAAGGAAAATCTGGTTATAAAGTGATGTCAGGATACAGTGGGACGTCGCCACAT
GAGAAAACCAGTGCTCGGAATCACAGACACTACCAGGATACAGCCTCAAGGCTCATCCACAACATGGACC
TGCGCACAATGACACAGTCGCTGGTGACTCTGGCGGAGGACAACATAGCCTTCTTCTCGAGCCAGGGTCC
TGGGGAAACGGCCCAGCGGCTGTCAGGCGTTTTTGCCGGTGTACGGGAGCAGGCGCTGGGGCTGGAGCCG
GCCCTGGGCCGCCTGCTGGGTGTGGCGCACCTCTTTGACCTGGACCCAGAGACACCGGCCAACGGGTACC
GCAGCCTAGTGCACACAGCCCGCTGCTGCCTGGCGCACCTCCTGCACAAATCCCGCTATGTGGCCTCCAA
CCGCCGCAGCATCTTCTTCCGCACCAGCCACAACCTGGCCGAGCTGGAGGCCTACCTGGCTGCCCTCACC
CAGCTCCGCGCTCTGGTCTACTACGCCCAGCGCCTGCTGGTTACCAATCGGCCGGGGGTACTCTTCTTTG
AGGGCGACGAGGGGCTCACCGCCGACTTCCTCCGGGAGTATGTCACGCTGCATAAGGGATGCTTCTATGG
CCGCTGCCTGGGCTTCCAGTTCACGCCTGCCATCCGGCCATTCCTGCAGACCATCTACATTGGGCTGGTG
TCCTTCGGGGAGCACTACAAACGCAACGAGACAGGCCTCAGTGTGGCCGCCAGCTCTCTCTTCACCAGCG
GCCGCTTTGCCATCGACCCCGAGCTGCGTGGGGCTGAGTTTGAGCGGATCACACAGAACCTGGACGTGCA
CTTCTGGAAAGCCTTCTGGAACATCACCGAGATGGAAGTGCTATCGTCTCTGGCCAACATGGCATCGGCC
ACCGTGAGGGTAAGCCGCCTGCTCAGCCTGCCACCCGAAGCCTTTGAGATGCCACTGACTGCCGACCCCA
CGCTCACGGTCACCATCTCACCCCCACTGGCCCACACAGGCCCTGGGCCCGTCCTCGTCAGGCTCATCTC
CTGTGACCTGCGTGAAGGACAGGACAGTGAGGAGCTCAGCAGCCTGATAAAGTCCAACGGCCAACGGAGC
CTGGAGCTGTGGCCGCGCCCCCAGCAGGCACCCCGCTCGCGGTCCCTGATAGTGCACTTCCACGGCGGTG
GCTTTGTGGCCCAGACCTCCAGATCCCACGAGCCCTACCTCAAGAGCTGGGCCCAGGAGCTGGGCGCCCC
CATCATCTCCATCGACTACTCCCTGGCCCCTGAGGCCCCCTTCCCCCGTGCGCTGGAGGAGTGCTTCTTC
GCCTACTGCTGGGCCATCAAGCACTGCGCCCTCCTTGGCTCAACAGGGGAACGAATCTGCCTTGCGGGGG
ACAGTGCAGGCGGGAACCTCTGCTTCACCGTGGCTCTTCGGGCAGCAGCCTACGGGGTGCGGGTGCCAGA
TGGCATCATGGCAGCCTACCCGGCCACAATGCTGCAGCCTGCCGCCTCTCCCTCCCGCCTGCTGAGCCTC
ATGGACCCCTTGCTGCCCCTCAGTGTGCTCTCCAAGTGTGTCAGCGCCTATGCTGGTGCAAAGACGGAGG
ACCACTCCAACTCAGACCAGAAAGCCCTCGGCATGATGGGGCTGGTGCGGCGGGACACAGCCCTGCTCCT
CCGAGACTTCCGCCTGGGTGCCTCCTCATGGCTCAACTCCTTCCTGGAGTTAAGTGGGCGCAAGTCCCAG
AAGATGTCGGAGCCCATAGCAGAGCCGATGCGCCGCAGTGTGTCTGAAGCAGCACTGGCCCAGCCCCAGG
GCCCACTGGGCACGGATTCCCTCAAGAACCTGACCCTGAGGGACTTGAGCCTGAGGGGAAACTCCGAGAC
GTCGTCGGACACCCCCGAGATGTCGCTGTCAGCTGAGACACTTAGCCCCTCCACACCCTCCGATGTCAAC
TTCTTATTACCACCTGAGGATGCAGGGGAAGAGGCTGAGGCCAAAAATGAGCTGAGCCCCATGGACAGAG
GCCTGGGCGTCCGTGCCGCCTTCCCCGAGGGTTTCCACCCCCGACGCTCCAGCCAGGGTGCCACACAGAT
GCCCCTCTACTCCTCACCCATAGTCAAGAACCCCTTCATGTCGCCGCTGCTGGCACCCGACAGCATGCTC
AAGAGCCTGCCACCTGTGCACATCGTGGCGTGCGCGCTGGACCCCATGCTGGACGACTCGGTCATGCTCG
CGCGGCGACTGCGCAACCTGGGCCAGCCGGTGACGCTGCGCGTGGTGGAGGACCTGCCGCACGGCTTCCT
GACCCTAGCGGCGCTGTGCCGCGAGACGCGCCAGGCCGCAGAGCTGTGCGTGGAGCGCATCCGCCTCGTC
CTCACTCCTCCCGCCGGAGCCGGGCCGAGCGGGGAGACGGGGGCTGCGGGGGTAGACGGGGGCTGCGGGG
GGCGACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209729 protein sequence
Red=Cloning site Green=Tags(s)

MEPGSKSVSRSDWQPEPHQRPITPLEPGPEKTPIAQPESKTLQGSNTQQKPASNQRPLTQQETPAQHDAE
SQKEPRAQQKSASQEEFLAPQKPAPQQSPYIQRVLLTQQEAASQQGPGLGKESITQQEPALRQRHVAQPG
PGPGEPPPAQQEAESTPAAQAKPGAKREPSAPTESTSQETPEQSDKQTTPVQGAKSKQGSLTELGFLTKL
QELSIQRSALEWKALSEWVTDSESESDVGSSSDTDSPATMGGMVAQGVKLGFKGKSGYKVMSGYSGTSPH
EKTSARNHRHYQDTASRLIHNMDLRTMTQSLVTLAEDNIAFFSSQGPGETAQRLSGVFAGVREQALGLEP
ALGRLLGVAHLFDLDPETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALT
QLRALVYYAQRLLVTNRPGVLFFEGDEGLTADFLREYVTLHKGCFYGRCLGFQFTPAIRPFLQTIYIGLV
SFGEHYKRNETGLSVAASSLFTSGRFAIDPELRGAEFERITQNLDVHFWKAFWNITEMEVLSSLANMASA
TVRVSRLLSLPPEAFEMPLTADPTLTVTISPPLAHTGPGPVLVRLISCDLREGQDSEELSSLIKSNGQRS
LELWPRPQQAPRSRSLIVHFHGGGFVAQTSRSHEPYLKSWAQELGAPIISIDYSLAPEAPFPRALEECFF
AYCWAIKHCALLGSTGERICLAGDSAGGNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSL
MDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQ
KMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVN
FLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSML
KSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLV
LTPPAGAGPSGETGAAGVDGGCGGRH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005357
ORF Size 3228 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005357.4
RefSeq Size 3829 bp
RefSeq ORF 3231 bp
Locus ID 3991
UniProt ID Q05469
Cytogenetics 19q13.2
Protein Pathways Insulin signaling pathway
MW 116.6 kDa
Gene Summary The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]
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