Cytochrome C (CYCS) (NM_018947) Human Tagged ORF Clone
CYCS (Myc-DDK-tagged)-Human cytochrome c, somatic (CYCS), nuclear gene encoding mitochondrial protein
|Product Name||Cytochrome C (CYCS) (NM_018947) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||CYC; HCS; THC4|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC209724 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC209724 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||315 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_018947.1, NM_018947.2, NM_018947.3, NM_018947.4, NM_018947.5, NP_061820.1|
|Protein Families||Druggable Genome|
|Protein Pathways||Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Huntington's disease, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Small cell lung cancer, Viral myocarditis|
|Gene Summary||This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome. [provided by RefSeq, Jul 2010]|
|cDNA Clone Resources|
|SC113344||CYCS (untagged)-Human cytochrome c, somatic (CYCS), nuclear gene encoding mitochondrial protein||
|RG209724||CYCS (GFP-tagged) - Human cytochrome c, somatic (CYCS), nuclear gene encoding mitochondrial protein||
|RC209724L1||Lenti ORF clone of Human cytochrome c, somatic (CYCS), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged||
|RC209724L2||Lenti ORF clone of Human cytochrome c, somatic (CYCS), nuclear gene encoding mitochondrial protein, mGFP tagged||
|RC209724L3||Lenti ORF clone of Human cytochrome c, somatic (CYCS), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged||
|RC209724L4||Lenti ORF clone of Human cytochrome c, somatic (CYCS), nuclear gene encoding mitochondrial protein, mGFP tagged||