HOXA13 (NM_000522) Human Tagged ORF Clone

CAT#: RC209698

HOXA13 (Myc-DDK-tagged)-Human homeobox A13 (HOXA13)

Product Images

Circular map for RC209698

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HOXA13
• Synonyms: HOX1; HOX1J
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209698 representing NM_000522
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCCTCCGTGCTCCTCCACCCCCGCTGGATCGAGCCCACCGTCATGTTTCTCTACGACAACGGCG
  GCGGCCTGGTGGCCGACGAGCTCAACAAGAACATGGAAGGGGCGGCGGCGGCTGCAGCAGCGGCTGCAGC
  GGCGGCGGCTGCCGGGGCCGGGGGCGGGGGCTTCCCCCACCCGGCGGCTGCGGCGGCAGGGGGCAACTTC
  TCGGTGGCGGCGGCGGCCGCGGCTGCGGCGGCGGCCGCGGCCAACCAGTGCCGCAACCTGATGGCGCACC
  CGGCGCCCTTGGCGCCAGGAGCCGCGTCCGCCTACAGCAGCGCCCCCGGGGAGGCGCCCCCGTCGGCTGC
  CGCCGCTGCTGCCGCGGCTGCCGCTGCAGCCGCCGCCGCCGCCGCCGCGTCGTCCTCGGGAGGTCCCGGC
  CCGGCGGGCCCGGCGGGCGCAGAGGCCGCCAAGCAATGCAGCCCCTGCTCGGCAGCGGCGCAGAGCTCGT
  CGGGGCCCGCGGCGCTGCCCTATGGCTACTTCGGCAGCGGCTACTACCCGTGCGCCCGCATGGGCCCGCA
  CCCCAACGCCATCAAGTCGTGCGCGCAGCCCGCCTCGGCCGCCGCCGCCGCCGCCTTCGCGGACAAGTAC
  ATGGATACCGCCGGCCCAGCTGCCGAGGAGTTCAGCTCCCGCGCTAAGGAGTTCGCCTTCTACCACCAGG
  GCTACGCAGCCGGGCCTTACCACCACCATCAGCCCATGCCTGGCTACCTGGATATGCCAGTGGTGCCGGG
  CCTCGGGGGCCCCGGCGAGTCGCGCCACGAACCCTTGGGTCTTCCCATGGAAAGCTACCAGCCCTGGGCG
  CTGCCCAACGGCTGGAACGGCCAAATGTACTGCCCCAAAGAGCAGGCGCAGCCTCCCCACCTCTGGAAGT
  CCACTCTGCCCGACGTGGTCTCCCATCCCTCGGATGCCAGCTCCTATAGGAGGGGGAGAAAGAAGCGCGT
  GCCTTATACCAAGGTGCAATTAAAAGAACTTGAACGGGAATACGCCACGAATAAATTCATTACTAAGGAC
  AAACGGAGGCGGATATCAGCCACGACGAATCTCTCTGAGCGGCAGGTCACAATCTGGTTCCAGAACAGGA
  GGGTTAAAGAGAAAAAAGTCATCAACAAACTGAAAACCACTAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209698 representing NM_000522
  Red=Cloning site Green=Tags(s)
  
  MTASVLLHPRWIEPTVMFLYDNGGGLVADELNKNMEGAAAAAAAAAAAAAAGAGGGGFPHPAAAAAGGNF
  SVAAAAAAAAAAAANQCRNLMAHPAPLAPGAASAYSSAPGEAPPSAAAAAAAAAAAAAAAAAASSSGGPG
  PAGPAGAEAAKQCSPCSAAAQSSSGPAALPYGYFGSGYYPCARMGPHPNAIKSCAQPASAAAAAAFADKY
  MDTAGPAAEEFSSRAKEFAFYHQGYAAGPYHHHQPMPGYLDMPVVPGLGGPGESRHEPLGLPMESYQPWA
  LPNGWNGQMYCPKEQAQPPHLWKSTLPDVVSHPSDASSYRRGRKKRVPYTKVQLKELEREYATNKFITKD
  KRRRISATTNLSERQVTIWFQNRRVKEKKVINKLKTTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000522
• ORF Size: 1164 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000522.5
• RefSeq Size: 2514 bp
• RefSeq ORF: 1167 bp
• Locus ID: 3209
• UniProt ID: P31271
• Cytogenetics: 7p15.2
• Domains: homeobox
• Protein Families: Transcription Factors
• MW: 39.7 kDa
• Gene Summary: In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Expansion of a polyalanine tract in the encoded protein can cause hand-foot-uterus syndrome, also known as hand-foot-genital syndrome. [provided by RefSeq, Jul 2008]