GPR75 (NM_006794) Human Tagged ORF Clone

CAT#: RC209675

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GPR75 (Myc-DDK-tagged)-Human G protein-coupled receptor 75 (GPR75)



  "NM_006794" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GPR75
Synonyms GPRchr2; WI31133
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209675 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTCAACAGGCCACCTTCAGGATGCCCCCAATGCCACCTCGCTCCATGTGCCTCACTCACAGGAAG
GAAACAGCACCTCTCTCCAGGAGGGTCTTCAGGATCTCATCCACACAGCCACCTTGGTGACCTGTACTTT
TCTACTGGCGGTCATCTTCTGCCTGGGTTCCTATGGCAACTTCATTGTCTTCTTGTCCTTCTTCGATCCA
GCCTTCAGGAAATTCAGAACCAACTTTGATTTCATGATCCTGAACCTGTCCTTCTGTGACCTCTTCATTT
GTGGAGTGACAGCCCCCATGTTCACCTTTGTGTTATTCTTCAGCTCAGCCAGTAGTATCCCGGATGCTTT
CTGCTTCACTTTCCATCTCACCAGTTCAGGCTTCATCATCATGTCTCTGAAGACAGTGGCAGTGATCGCC
CTGCACCGGCTCCGGATGGTGTTGGGGAAACAGCCTAATCGCACGGCCTCCTTTCCCTGCACCGTACTCC
TCACCCTGCTTCTCTGGGCCACCAGTTTCACCCCTGCCACCTTGGCTACCTTGAAAACCAGCAAGTCCCA
CCTCTGTCTTCCCATGTCCAGTCTGATTGCTGGAAAAGGGAAAGCCATTTTGTCTCTCTATGTGGTCGAC
TTCACCTTCTGTGTTGCTGTGGTCTCTGTCTCTTACATCATGATTGCTCAGACCCTGCGGAAGAACGCTC
AAGTCAGAAAGTGCCCCCCTGTAATCACAGTCGATGCTTCCAGACCACAGCCTTTCATGGGGGTCCCTGT
GCAGGGAGGTGGAGATCCCATCCAGTGTGCCATGCCGGCTCTGTATAGGAACCAGAATTACGACAAACTG
CAGCACGTTCAGACCCGTGGATATACCAAGAGTCCCAACCAACTGGTCACCCCTGCAGCAAGCCGACTCC
AGCTCGTATCAGCCATCAACCTCTCCACTGCCAAGGATTCCAAAGCCGTGGTCACCTGTGTGATCATTGT
GCTGTCAGTCCTGGTGTGCTGTCTTCCACTGGGGATTTCCTTGGTACAGGTGGTTCTCTCCAGCAATGGG
AGCTTCATTCTTTACCAGTTTGAATTGTTTGGATTTACTCTTATATTTTTCAAGTCAGGATTAAACCCTT
TTATATATTCTCGGAACAGTGCAGGGCTGAGAAGGAAAGTGCTCTGGTGCCTCCAATACATAGGCCTGGG
TTTTTTCTGCTGCAAACAAAAGACTCGACTTCGAGCCATGGGAAAAGGGAACCTCGAAGTCAACAGAAAC
AAATCCTCCCATCATGAAACAAACTCTGCCTACATGTTATCTCCAAAGCCACAGAAGAAATTTGTGGACC
AGGCTTGTGGCCCAAGTCATTCAAAAGAAAGTATGGTGAGTCCCAAGATCTCTGCTGGACATCAACACTG
TGGTCAGAGCAGCTCGACCCCCATCAACACTCGGATTGAACCTTACTACAGCATCTATAACAGCAGCCCT
TCCCAGGAGGAGAGCAGCCCATGTAACTTACAGCCAGTAAACTCTTTTGGATTTGCCAATTCATATATTG
CCATGCATTATCACACCACTAATGACTTAGTGCAGGAATATGACAGCACTTCAGCCAAGCAGATTCCAGT
CCCCTCCGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209675 protein sequence
Red=Cloning site Green=Tags(s)

MNSTGHLQDAPNATSLHVPHSQEGNSTSLQEGLQDLIHTATLVTCTFLLAVIFCLGSYGNFIVFLSFFDP
AFRKFRTNFDFMILNLSFCDLFICGVTAPMFTFVLFFSSASSIPDAFCFTFHLTSSGFIIMSLKTVAVIA
LHRLRMVLGKQPNRTASFPCTVLLTLLLWATSFTPATLATLKTSKSHLCLPMSSLIAGKGKAILSLYVVD
FTFCVAVVSVSYIMIAQTLRKNAQVRKCPPVITVDASRPQPFMGVPVQGGGDPIQCAMPALYRNQNYDKL
QHVQTRGYTKSPNQLVTPAASRLQLVSAINLSTAKDSKAVVTCVIIVLSVLVCCLPLGISLVQVVLSSNG
SFILYQFELFGFTLIFFKSGLNPFIYSRNSAGLRRKVLWCLQYIGLGFFCCKQKTRLRAMGKGNLEVNRN
KSSHHETNSAYMLSPKPQKKFVDQACGPSHSKESMVSPKISAGHQHCGQSSSTPINTRIEPYYSIYNSSP
SQEESSPCNLQPVNSFGFANSYIAMHYHTTNDLVQEYDSTSAKQIPVPSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006794
ORF Size 1620 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006794.4
RefSeq Size 2159 bp
RefSeq ORF 1623 bp
Locus ID 10936
UniProt ID O95800
Cytogenetics 2p16.2
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
MW 59.3 kDa
Gene Summary GPR75 is a member of the G protein-coupled receptor family. GPRs are cell surface receptors that activate guanine-nucleotide binding proteins upon the binding of a ligand.[supplied by OMIM, Jul 2002]

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