SFRS3 (SRSF3) (NM_003017) Human Tagged ORF Clone
SRSF3 (Myc-DDK-tagged)-Human serine/arginine-rich splicing factor 3 (SRSF3), transcript variant 1
|Product Name||SFRS3 (SRSF3) (NM_003017) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC209660 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC209660 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||492 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_003017.1, NM_003017.2, NM_003017.3, NM_003017.4, NP_003008.1|
|RefSeq Size||3144 bp|
|RefSeq ORF||495 bp|
|Protein Families||Stem cell - Pluripotency|
|Gene Summary||The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010]|
|cDNA Clone Resources|
|SC118249||SRSF3 (untagged)-Human serine/arginine-rich splicing factor 3 (SRSF3), transcript variant 1||
|RG209660||SRSF3 (GFP-tagged) - Human serine/arginine-rich splicing factor 3 (SRSF3), transcript variant 1||
|RC209660L1||Lenti ORF clone of Human serine/arginine-rich splicing factor 3 (SRSF3), transcript variant 1, Myc-DDK-tagged||
|RC209660L2||Lenti ORF clone of Human serine/arginine-rich splicing factor 3 (SRSF3), transcript variant 1, mGFP tagged||
|RC209660L3||Lenti ORF clone of Human serine/arginine-rich splicing factor 3 (SRSF3), transcript variant 1, Myc-DDK-tagged||
|RC209660L4||Lenti ORF clone of Human serine/arginine-rich splicing factor 3 (SRSF3), transcript variant 1, mGFP tagged||