IMP3 (IGF2BP3) (NM_006547) Human Tagged ORF Clone

CAT#: RC209597

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IGF2BP3 (Myc-DDK-tagged)-Human insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3)



  "NM_006547" in other vectors (7)

Reconstitution Protocol

USD 808.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol IGF2BP3
Synonyms CT98; IMP-3; IMP3; KOC; KOC1; VICKZ3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209597 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAACTGTATATCGGAAACCTCAGCGAGAACGCCGCCCCCTCGGACCTAGAAAGTATCTTCAAGG
ACGCCAAGATCCCGGTGTCGGGACCCTTCCTGGTGAAGACTGGCTACGCGTTCGTGGACTGCCCGGACGA
GAGCTGGGCCCTCAAGGCCATCGAGGCGCTTTCAGGTAAAATAGAACTGCACGGGAAACCCATAGAAGTT
GAGCACTCGGTCCCAAAAAGGCAAAGGATTCGGAAACTTCAGATACGAAATATCCCGCCTCATTTACAGT
GGGAGGTGCTGGATAGTTTACTAGTCCAGTATGGAGTGGTGGAGAGCTGTGAGCAAGTGAACACTGACTC
GGAAACTGCAGTTGTAAATGTAACCTATTCCAGTAAGGACCAAGCTAGACAAGCACTAGACAAACTGAAT
GGATTTCAGTTAGAGAATTTCACCTTGAAAGTAGCCTATATCCCTGATGAAATGGCCGCCCAGCAAAACC
CCTTGCAGCAGCCCCGAGGTCGCCGGGGGCTTGGGCAGAGGGGCTCCTCAAGGCAGGGGTCTCCAGGATC
CGTATCCAAGCAGAAACCATGTGATTTGCCTCTGCGCCTGCTGGTTCCCACCCAATTTGTTGGAGCCATC
ATAGGAAAAGAAGGTGCCACCATTCGGAACATCACCAAACAGACCCAGTCTAAAATCGATGTCCACCGTA
AAGAAAATGCGGGGGCTGCTGAGAAGTCGATTACTATCCTCTCTACTCCTGAAGGCACCTCTGCGGCTTG
TAAGTCTATTCTGGAGATTATGCATAAGGAAGCTCAAGATATAAAATTCACAGAAGAGATCCCCTTGAAG
ATTTTAGCTCATAATAACTTTGTTGGACGTCTTATTGGTAAAGAAGGAAGAAATCTTAAAAAAATTGAGC
AAGACACAGACACTAAAATCACGATATCTCCATTGCAGGAATTGACGCTGTATAATCCAGAACGCACTAT
TACAGTTAAAGGCAATGTTGAGACATGTGCCAAAGCTGAGGAGGAGATCATGAAGAAAATCAGGGAGTCT
TATGAAAATGATATTGCTTCTATGAATCTTCAAGCACATTTAATTCCTGGATTAAATCTGAACGCCTTGG
GTCTGTTCCCACCCACTTCAGGGATGCCACCTCCCACCTCAGGGCCCCCTTCAGCCATGACTCCTCCCTA
CCCGCAGTTTGAGCAATCAGAAACGGAGACTGTTCATCTGTTTATCCCAGCTCTATCAGTCGGTGCCATC
ATCGGCAAGCAGGGCCAGCACATCAAGCAGCTTTCTCGCTTTGCTGGAGCTTCAATTAAGATTGCTCCAG
CAGAAGCACCAGATGCTAAAGTGAGGATGGTGATTATCACTGGACCACCAGAGGCTCAGTTCAAGGCTCA
GGGAAGAATTTATGGAAAAATTAAAGAAGAAAACTTTGTTAGTCCTAAAGAAGAGGTGAAACTTGAAGCT
CATATCAGAGTGCCATCCTTTGCTGCTGGCAGAGTTATTGGAAAAGGAGGCAAAACGGTGAATGAACTTC
AGAATTTGTCAAGTGCAGAAGTTGTTGTCCCTCGTGACCAGACACCTGATGAGAATGACCAAGTGGTTGT
CAAAATAACTGGTCACTTCTATGCTTGCCAGGTTGCCCAGAGAAAAATTCAGGAAATTCTGACTCAGGTA
AAGCAGCACCAACAACAGAAGGCTCTGCAAAGTGGACCACCTCAGTCAAGACGGAAG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC209597 protein sequence
Red=Cloning site Green=Tags(s)

MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGKIELHGKPIEV
EHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETAVVNVTYSSKDQARQALDKLN
GFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSSRQGSPGSVSKQKPCDLPLRLLVPTQFVGAI
IGKEGATIRNITKQTQSKIDVHRKENAGAAEKSITILSTPEGTSAACKSILEIMHKEAQDIKFTEEIPLK
ILAHNNFVGRLIGKEGRNLKKIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRES
YENDIASMNLQAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAI
IGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFVSPKEEVKLEA
HIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKITGHFYACQVAQRKIQEILTQV
KQHQQQKALQSGPPQSRRK

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006547
ORF Size 1737 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006547.3
RefSeq Size 4168 bp
RefSeq ORF 1740 bp
Locus ID 10643
UniProt ID O00425
Cytogenetics 7p15.3
Domains RRM, KH
MW 63.7 kDa
Gene Summary The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]

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