PNPLA3 (NM_025225) Human Tagged ORF Clone

CAT#: RC209577

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PNPLA3 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 3 (PNPLA3)



  "NM_025225" in other vectors (6)

Reconstitution Protocol

USD 457.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PNPLA3
Synonyms ADPN; C22orf20; iPLA(2)epsilon
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209577 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACGACGCAGAGCGCGGCTGGAGCTTGTCCTTCGCGGGCTGCGGCTTCCTGGGCTTCTACCACGTCG
GGGCGACCCGCTGCCTGAGCGAGCACGCCCCGCACCTCCTCCGCGACGCGCGCATGTTGTTCGGCGCTTC
GGCCGGGGCGTTGCACTGCGTCGGCGTCCTCTCCGGTATCCCGCTGGAGCAGACTCTGCAGGTCCTCTCA
GATCTTGTGCGGAAGGCCAGGAGTCGGAACATTGGCATCTTCCATCCATCCTTCAACTTAAGCAAGTTCC
TCCGACAGGGTCTCGGCAAATGCCTCCCGGCCAATGTCCACCAGCTCATCTCCGGCAAAATAGGCATCTC
TCTTACCAGAGTGTCTGATGGGGAAAACGTTCTGGTGTCTGACTTTCGGTCCAAAGACGAAGTCGTGGAT
GCCTTGGTATGTTCCTGCTTCATGCCTTTCTACAGTGGCCTTATCCCTCCTTCCTTCAGAGGCGTGCGAT
ATGTGGATGGAGGAGTGAGTGACAACGTACCCTTCATTGATGCCAAAACAACCATCACCGTGTCCCCCTT
CTATGGGGAGTACGACATCTGCCCTAAAGTCAAGTCCACGAACTTTCTTCATGTGGACATCACCAAGCTC
AGTCTACGCCTCTGCACAGGGAACCTCTACCTTCTCTCGAGAGCTTTTGTCCCCCCGGATCTCAAGGTGC
TGGGAGAGATATGCCTTCGAGGATATTTGGATGCATTCAGGTTCTTGGAAGAGAAGGGCATCTGCAACAG
GCCCCAGCCAGGCCTGAAGTCATCCTCAGAAGGGATGGATCCTGAGGTCGCCATGCCCAGCTGGGCAAAC
ATGAGTCTGGATTCTTCCCCGGAGTCGGCTGCCTTGGCTGTGAGGCTGGAGGGAGATGAGCTGCTAGACC
ACCTGCGTCTCAGCATCCTGCCCTGGGATGAGAGCATCCTGGACACCCTCTCGCCCAGGCTCGCTACAGC
ACTGAGTGAAGAAATGAAAGACAAAGGTGGATACATGAGCAAGATTTGCAACTTGCTACCCATTAGGATA
ATGTCTTATGTAATGCTGCCCTGTACCCTGCCTGTGGAATCTGCCATTGCGATTGTCCAGAGACTGGTGA
CATGGCTTCCAGATATGCCCGACGATGTCCTGTGGTTGCAGTGGGTGACCTCACAGGTGTTCACTCGAGT
GCTGATGTGTCTGCTCCCCGCCTCCAGGTCCCAAATGCCAGTGAGCAGCCAACAGGCCTCCCCATGCACA
CCTGAGCAGGACTGGCCCTGCTGGACTCCCTGCTCCCCCGAGGGCTGTCCAGCAGAGACCAAAGCAGAGG
CCACCCCGCGGTCCATCCTCAGGTCCAGCCTGAACTTCTTCTTGGGCAATAAAGTACCTGCTGGTGCTGA
GGGGCTCTCCACCTTTCCCAGTTTTTCACTAGAGAAGAGTCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209577 protein sequence
Red=Cloning site Green=Tags(s)

MYDAERGWSLSFAGCGFLGFYHVGATRCLSEHAPHLLRDARMLFGASAGALHCVGVLSGIPLEQTLQVLS
DLVRKARSRNIGIFHPSFNLSKFLRQGLGKCLPANVHQLISGKIGISLTRVSDGENVLVSDFRSKDEVVD
ALVCSCFMPFYSGLIPPSFRGVRYVDGGVSDNVPFIDAKTTITVSPFYGEYDICPKVKSTNFLHVDITKL
SLRLCTGNLYLLSRAFVPPDLKVLGEICLRGYLDAFRFLEEKGICNRPQPGLKSSSEGMDPEVAMPSWAN
MSLDSSPESAALAVRLEGDELLDHLRLSILPWDESILDTLSPRLATALSEEMKDKGGYMSKICNLLPIRI
MSYVMLPCTLPVESAIAIVQRLVTWLPDMPDDVLWLQWVTSQVFTRVLMCLLPASRSQMPVSSQQASPCT
PEQDWPCWTPCSPEGCPAETKAEATPRSILRSSLNFFLGNKVPAGAEGLSTFPSFSLEKSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_025225
ORF Size 1443 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_025225.3
RefSeq Size 2805 bp
RefSeq ORF 1446 bp
Locus ID 80339
UniProt ID Q9NST1
Cytogenetics 22q13.31
Domains Patatin
Protein Pathways Glycerolipid metabolism, Glycerophospholipid metabolism, Limonene and pinene degradation, Metabolic pathways, Phenylalanine metabolism, Tyrosine metabolism
MW 52.8 kDa
Gene Summary The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008]

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