Galectin 2 (LGALS2) (NM_006498) Human Tagged ORF Clone

CAT#: RC209552

LGALS2 (Myc-DDK-tagged)-Human lectin, galactoside-binding, soluble, 2 (LGALS2)

Product Images

Circular map for RC209552

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LGALS2 (Cat# RC209552, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS2(Cat# TA803291). Positive lysates LY416599 (100ug) and LC416599 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416599) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209552 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LGALS2 protein (Cat# TP309552). The protein was produced from HEK293T cells transfected with LGALS2 cDNA clone (Cat# RC209552) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Galectin 2
• Synonyms: HL14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209552 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGGGGAACTTGAGGTTAAGAACATGGACATGAAGCCGGGGTCAACCCTGAAGATCACAGGCAGCA
  TCGCCGATGGCACTGATGGCTTTGTAATTAATCTGGGCCAGGGGACAGACAAGCTGAACCTGCATTTCAA
  CCCTCGCTTCAGCGAATCCACCATTGTCTGCAACTCATTGGACGGCAGCAACTGGGGGCAAGAACAACGG
  GAAGATCACCTGTGCTTCAGCCCAGGGTCAGAGGTCAAGTTCACAGTGACCTTTGAGAGTGACAAATTCA
  AGGTGAAGCTGCCAGATGGGCACGAGCTGACTTTTCCCAACAGGCTGGGTCACAGCCACCTGAGCTACCT
  GAGCGTAAGGGGCGGGTTCAACATGTCCTCTTTCAAGTTAAAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209552 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQR
  EDHLCFSPGSEVKFTVTFESDKFKVKLPDGHELTFPNRLGHSHLSYLSVRGGFNMSSFKLKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006498
• ORF Size: 396 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006498.3
• RefSeq Size: 543 bp
• RefSeq ORF: 399 bp
• Locus ID: 3957
• UniProt ID: P05162
• Cytogenetics: 22q13.1
• Domains: Gal-bind_lectin
• MW: 14.6 kDa
• Gene Summary: The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. A single nucleotide polymorphism in an intron of this gene can alter the transcriptional level of the protein, with a resultant increased risk of myocardial infarction. [provided by RefSeq, Jul 2008]