GTF2H2 (NM_001515) Human Tagged ORF Clone

CAT#: RC209543

GTF2H2 (Myc-DDK-tagged)-Human general transcription factor IIH, polypeptide 2, 44kDa (GTF2H2)

Product Images

Circular map for RC209543

• 

  Western blot validation of overexpression lysate (Cat# LY419885) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209543 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTF2H2
• Synonyms: BTF2; BTF2 p44; BTF2P44; p44; T-BTF2P44; TFIIH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209543 representing NM_001515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAAGAACCTGAAAGAACTAAGCGATGGGAAGGAGGCTATGAAAGAACATGGGAGATTCTTAAAG
  AAGATGAATCTGGATCACTTAAAGCTACAATAGAAGACATTCTATTCAAGGCAAAGAGAAAAAGAGTATT
  TGAGCACCATGGACAAGTTCGACTTGGAATGATGCGCCACCTTTATGTGGTAGTAGATGGATCAAGAACA
  ATGGAAGACCAAGATTTAAAGCCTAATAGACTGACGTGTACTTTAAAGTTGTTGGAATACTTTGTAGAGG
  AATATTTTGATCAAAATCCTATTAGTCAGATTGGAATAATTGTAACTAAGAGTAAAAGAGCTGAAAAATT
  GACTGAACTTTCAGGAAACCCAAGAAAACATATAACGTCTTTGAAGGAAGCTGTGGATATGACCTGCCAT
  GGAGAGCCATCTCTTTATAATTCCCTAAGCATGGCTATGCAGACTCTAAAACACATGCCTGGACATACAA
  GTCGAGAAGTACTAATCATCTTTAGCAGCCTTACAACTTGCGATCCATCTAATATTTATGATTTAATCAA
  GACCCTAAAGGCAGCTAAAATTAGAGTATCTGTTATTGGATTGTCTGCAGAAGTTCGCGTTTGCACTGTA
  CTTGCTCGTGAAACTGGTGGCACGTACCATGTTATTTTAGATGAAAGCCATTACAAAGAGTTGCTCACAC
  ATCATCTTAGTCCTCCTCCTGCTAGCTCAAGTTCTGAATGCTCACTTATTCGTATGGGATTTCCTCAGCA
  CACCATTGCTTCTTTATCTGACCAGGATGCAAAACCCTCTTTCAGCATGGCGCATTTGGATGGCAATACT
  GAGCCAGGGCTTACATTAGGAGGCTATTTCTGCCCACAGTGTCGGGCAAAGTACTGTGAGCTACCTGTTG
  AATGTAAAATCTGTGGTCTTACTTTGGTGTCTGCTCCCCACTTGGCACGGTCTTACCATCATTTGTTTCC
  TTTGGATGCTTTTCAAGAAATTCCCCTAGAAGAATATAATGGAGAAAGATTTTGTTATGGATGTCAGGGG
  GAATTGAAAGACCAACATGTTTATGTTTGTGCTGTGTGCCAAAATGTTTTCTGTGTGGACTGTGATGTTT
  TTGTTCATGATTCTCTACACTGTTGCCCTGGCTGTATTCATAAGATTCCAGCTCCTTCAGGTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209543 representing NM_001515
  Red=Cloning site Green=Tags(s)
  
  MDEEPERTKRWEGGYERTWEILKEDESGSLKATIEDILFKAKRKRVFEHHGQVRLGMMRHLYVVVDGSRT
  MEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKRAEKLTELSGNPRKHITSLKEAVDMTCH
  GEPSLYNSLSMAMQTLKHMPGHTSREVLIIFSSLTTCDPSNIYDLIKTLKAAKIRVSVIGLSAEVRVCTV
  LARETGGTYHVILDESHYKELLTHHLSPPPASSSSECSLIRMGFPQHTIASLSDQDAKPSFSMAHLDGNT
  EPGLTLGGYFCPQCRAKYCELPVECKICGLTLVSAPHLARSYHHLFPLDAFQEIPLEEYNGERFCYGCQG
  ELKDQHVYVCAVCQNVFCVDCDVFVHDSLHCCPGCIHKIPAPSGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001515
• ORF Size: 1185 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001515.3, NP_001506.1
• RefSeq Size: 1951 bp
• RefSeq ORF: 1188 bp
• Locus ID: 2966
• UniProt ID: Q13888
• Cytogenetics: 5q13.2
• Domains: VWA, Ssl1
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Basal transcription factors, Nucleotide excision repair
• MW: 44.2 kDa
• Gene Summary: This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however, no sequence data is currently available for the centromeric copy of the gene. [provided by RefSeq, Jul 2008]