ZCCHC8 (NM_017612) Human Tagged ORF Clone

CAT#: RC209528

ZCCHC8 (Myc-DDK-tagged)-Human zinc finger, CCHC domain containing 8 (ZCCHC8)

Product Images

Circular map for RC209528

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  Western blot validation of overexpression lysate (Cat# LY413673) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209528 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZCCHC8 protein (Cat# TP309528). The protein was produced from HEK293T cells transfected with ZCCHC8 cDNA clone (Cat# RC209528) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZCCHC8
• Synonyms: PFBMFT5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209528 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCAGAGGTGTATTTTGGCGATCTAGAGCTCTTCGAGCCGTTCGACCACCCAGAGGAGTCGATTC
  CGAAGCCCGTTCACACTCGCTTCAAGGACGACGACGGCGACGAGGAGGACGAAAATGGGGTCGGCGACGC
  GGAGCTACGGGAGCGGCTTCGGCAGTGCGAGGAGACCATCGAGCAGCTCCGCGCCGAGAATCAAGAACTT
  AAACGAAAATTGAACATTCTGACTCGACCGAGTGGAATATTGGTGAACGATACTAAGTTAGATGGACCTA
  TATTACAGATTCTATTCATGAACAATGCTATTTCAAAGCAATATCATCAAGAAATAGAGGAATTTGTATC
  AAATTTAGTAAAAAGATTTGAGGAACAGCAGAAAAATGATGTGGAAAAGACTTCCTTTAATCTTTTGCCC
  CAGCCATCCAGTATTGTGCTAGAGGAGGACCACAAAGTGGAAGAGTCCTGTGCCATTAAAAACAACAAGG
  AAGCTTTCAGTGTTGTAGGAAGTGTCCTGTATTTTACTAATTTTTGCCTTGATAAATTGGGGCAACCGCT
  TCTAAATGAAAACCCTCAGCTTTCCGAAGGATGGGAAATACCCAAGTACCATCAAGTCTTCAGCCACATT
  GTTTCTCTAGAAGGGCAAGAAATACAAGTAAAGGCAAAAAGGCCAAAGCCTCACTGTTTCAATTGTGGTT
  CTGAAGAACACCAAATGAAAGATTGCCCAATGCCTCGGAATGCTGCTCGAATAAGTGAAAAGAGAAAAGA
  GTATATGGATGCCTGTGGAGAAGCAAACAATCAGAATTTCCAGCAGCGATACCACGCAGAAGAAGTAGAA
  GAAAGATTTGGAAGATTCAAGCCAGGAGTTATTAGTGAGGAACTTCAAGATGCACTAGGTGTGACAGACA
  AGAGTCTTCCACCTTTTATCTATCGGATGCGCCAGCTAGGGTACCCACCAGGGTGGCTCAAAGAGGTTGA
  ATTGGAGAATTCGGGGCTTGCACTCTATGATGGAAAAGATGGCACTGATGGGGAAACAGAAGTTGGAGAA
  ATACAACAGAATAAAAGTGTCACTTACGATCTCTCAAAATTGGTCAACTATCCTGGTTTTAATATATCTA
  CTCCCAGAGGAATTCCAGACGAATGGAGGATCTTTGGTTCCATACCAATGCAGGCATGTCAGCAGAAGGA
  TGTGTTTGCCAATTACCTTACTTCTAACTTCCAAGCGCCAGGTGTGAAGTCTGGCAACAAGAGGTCTTCA
  TCTCACTCTAGCCCAGGTAGTCCAAAGAAGCAGAAGAATGAAAGCAACTCAGCGGGATCTCCCGCCGACA
  TGGAGCTCGATTCAGATATGGAGGTACCACATGGTTCTCAGAGCAGCGAAAGTTTTCAGTTTCAACCACC
  ATTACCTCCTGACACTCCTCCACTCCCCCGGGGAACTCCTCCACCCGTCTTCACCCCTCCACTCCCAAAG
  GGCACCCCGCCGCTGACTCCCAGTGACTCACCCCAGACCAGAACAGCATCTGGAGCTGTGGATGAGGACG
  CACTGACTCTAGAAGAACTTGAAGAACAGCAGAGGCGGATCTGGGCAGCTCTTGAGCAGGCCGAGAGCGT
  AAACAGCGACTCCGACGTTCCTGTGGACACACCTTTAACTGGCAATTCCGTTGCCTCATCACCTTGTCCA
  AATGAGCTAGACCTCCCTGTCCCGGAGGGAAAAACATCTGAAAAGCAGACGCTGGATGAGCCTGAGGTAC
  CAGAGATTTTTACAAAGAAATCAGAAGCTGGACATGCCTCCAGTCCAGACTCTGAGGTGACATCACTTTG
  TCAGAAGGAAAAAGCAGAGTTGGCTCCGGTAAACACTGAAGGTGCCCTTCTTGATAATGGCAGTGTCGTA
  CCAAACTGTGACATCAGCAATGGGGGCAGCCAGAAGCTCTTTCCTGCAGACACCAGTCCTTCAACGGCCA
  CTAAAATTCATAGCCCTATACCTGACATGAGCAAATTTGCAACTGGAATCACGCCATTTGAATTTGAGAA
  TATGGCAGAATCTACTGGAATGTACCTCAGGATAAGAAGCTTGTTAAAGAACTCACCCCGAAACCAGCAG
  AAAAACAAAAAGGCCTCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209528 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAEVYFGDLELFEPFDHPEESIPKPVHTRFKDDDGDEEDENGVGDAELRERLRQCEETIEQLRAENQEL
  KRKLNILTRPSGILVNDTKLDGPILQILFMNNAISKQYHQEIEEFVSNLVKRFEEQQKNDVEKTSFNLLP
  QPSSIVLEEDHKVEESCAIKNNKEAFSVVGSVLYFTNFCLDKLGQPLLNENPQLSEGWEIPKYHQVFSHI
  VSLEGQEIQVKAKRPKPHCFNCGSEEHQMKDCPMPRNAARISEKRKEYMDACGEANNQNFQQRYHAEEVE
  ERFGRFKPGVISEELQDALGVTDKSLPPFIYRMRQLGYPPGWLKEVELENSGLALYDGKDGTDGETEVGE
  IQQNKSVTYDLSKLVNYPGFNISTPRGIPDEWRIFGSIPMQACQQKDVFANYLTSNFQAPGVKSGNKRSS
  SHSSPGSPKKQKNESNSAGSPADMELDSDMEVPHGSQSSESFQFQPPLPPDTPPLPRGTPPPVFTPPLPK
  GTPPLTPSDSPQTRTASGAVDEDALTLEELEEQQRRIWAALEQAESVNSDSDVPVDTPLTGNSVASSPCP
  NELDLPVPEGKTSEKQTLDEPEVPEIFTKKSEAGHASSPDSEVTSLCQKEKAELAPVNTEGALLDNGSVV
  PNCDISNGGSQKLFPADTSPSTATKIHSPIPDMSKFATGITPFEFENMAESTGMYLRIRSLLKNSPRNQQ
  KNKKASE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017612
• ORF Size: 2121 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017612.5
• RefSeq Size: 4260 bp
• RefSeq ORF: 2124 bp
• Locus ID: 55596
• UniProt ID: Q6NZY4
• Cytogenetics: 12q24.31
• Domains: PSP
• MW: 78.6 kDa
• Gene Summary: This gene encodes a scaffold protein which serves as an assessory factor to the nuclear RNA exosome complex. The encoded protein forms a trimeric human nuclear exosome targeting (NEXT) complex, together with hMTR4 and the RNA-binding factor RBM7 which promotes the exosomal degradation of non-coding promoter-upstream transcripts, enhancer RNAs and 3'-extended products of histone- and small nuclear RNA transcription. This complex is also thought to recruit the exosome to degrade intronic RNAs via its interaction with both the exosome and the spliceosome. It contains both an N-terminal zinc-knuckle domain and a C-terminal proline-rich domain. [provided by RefSeq, Apr 2017]