SRPR beta (SRPRB) (NM_021203) Human Tagged ORF Clone

CAT#: RC209486

SRPRB (Myc-DDK-tagged)-Human signal recognition particle receptor, B subunit (SRPRB)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SRPRB (Cat# RC209486, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRPRB(Cat# TA504712). Positive lysates LY402853 (100ug) and LC402853 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY402853) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209486 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SRPRB protein (Cat# TP309486). The protein was produced from HEK293T cells transfected with SRPRB cDNA clone (Cat# RC209486) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRPR beta
• Synonyms: APMCF1; SR-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209486 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCGCGGACTCGCGCCGGCTGGCAGATGGCGGCGGTGCCGGGGGCACCTTCCAGCCCTACCTAG
  ACACCTTGCGGCAGGAGCTGCAGCAGACGGACCCAACGCTGTTGTCAGTAGTGGTGGCGGTTCTTGCGGT
  GCTGCTGACGCTAGTCTTCTGGAAGTTAATCCGGAGCAGAAGGAGCAGTCAGAGAGCTGTTCTTCTTGTT
  GGCCTTTGTGATTCCGGGAAAACGTTGCTCTTTGTCAGGTTGTTAACAGGCCTTTATAGAGACACTCAGA
  CGTCCATTACTGACAGCTGTGCTGTATACAGAGTCAACAATAACAGGGGCAATAGTCTGACCTTGATTGA
  CCTTCCCGGCCATGAGAGTTTGAGGCTTCAGTTCTTAGAGCGGTTTAAGTCTTCAGCCAGGGCTATTGTG
  TTTGTTGTGGATAGTGCAGCATTCCAGCGAGAGGTGAAAGATGTGGCTGAGTTTCTGTATCAAGTCCTCA
  TTGACAGTATGGGTCTGAAGAATACACCATCATTCTTAATAGCCTGCAATAAGCAAGATATTGCAATGGC
  AAAATCAGCAAAGTTAATTCAACAGCAGCTGGAGAAAGAACTCAACACCTTACGAGTTACCCGTTCTGCT
  GCCCCCAGCACACTGGACAGTTCCAGCACTGCCCCTGCTCAGCTGGGGAAGAAAGGCAAAGAGTTTGAAT
  TCTCACAGTTGCCCCTCAAAGTGGAGTTCCTGGAGTGCAGTGCCAAGGGTGGAAGAGGGGACGTGGGCTC
  TGCTGACATCCAGGACTTGGAGAAATGGCTGGCTAAAATTGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209486 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASADSRRLADGGGAGGTFQPYLDTLRQELQQTDPTLLSVVVAVLAVLLTLVFWKLIRSRRSSQRAVLLV
  GLCDSGKTLLFVRLLTGLYRDTQTSITDSCAVYRVNNNRGNSLTLIDLPGHESLRLQFLERFKSSARAIV
  FVVDSAAFQREVKDVAEFLYQVLIDSMGLKNTPSFLIACNKQDIAMAKSAKLIQQQLEKELNTLRVTRSA
  APSTLDSSSTAPAQLGKKGKEFEFSQLPLKVEFLECSAKGGRGDVGSADIQDLEKWLAKIA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021203
• ORF Size: 813 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021203.4
• RefSeq Size: 2880 bp
• RefSeq ORF: 816 bp
• Locus ID: 58477
• UniProt ID: Q9Y5M8
• Cytogenetics: 3q22.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 29.7 kDa
• Gene Summary: The protein encoded by this gene has similarity to mouse protein which is a subunit of the signal recognition particle receptor (SR). This subunit is a transmembrane GTPase belonging to the GTPase superfamily. It anchors alpha subunit, a peripheral membrane GTPase, to the ER membrane. SR is required for the cotranslational targeting of both secretory and membrane proteins to the ER membrane. [provided by RefSeq, Jul 2008]