VGF (NM_003378) Human Tagged ORF Clone

CAT#: RC209477

VGF (Myc-DDK-tagged)-Human VGF nerve growth factor inducible (VGF)

Product Images

Circular map for RC209477

• 

  Western blot validation of overexpression lysate (Cat# LY418733) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209477 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified VGF protein (Cat# TP309477). The protein was produced from HEK293T cells transfected with VGF cDNA clone (Cat# RC209477) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VGF
• Synonyms: SCG7; SgVII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209477 representing NM_003378
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCCCTCAGATTGTCGGCTTCCGCCCTCTTCTGCCTTCTGCTGATCAACGGGTTAGGGGCAGCAC
  CCCCTGGTCGCCCTGAGGCGCAGCCTCCTCCTCTCAGCTCTGAGCATAAAGAGCCGGTAGCCGGGGACGC
  AGTGCCCGGGCCAAAGGATGGCAGCGCCCCAGAGGTCCGAGGCGCTCGGAATTCCGAGCCGCAGGACGAG
  GGAGAGCTTTTCCAGGGCGTGGATCCCCGGGCGCTGGCCGCGGTGCTGCTGCAGGCACTCGACCGTCCCG
  CCTCACCCCCGGCACCAAGCGGCTCCCAGCAGGGGCCGGAGGAAGAAGCAGCTGAAGCTCTGCTGACCGA
  GACCGTGCGCAGCCAGACCCACAGCCTCCCGGCGCCGGAGAGCCCGGAGCCCGCGGCTCCGCCTCGCCCT
  CAGACTCCGGAGAATGGGCCCGAGGCGAGCGATCCCTCCGAGGAGCTCGAGGCGCTAGCGTCCCTGCTCC
  AGGAACTGCGAGATTTCAGTCCAAGTAGCGCCAAGCGCCAGCAGGAGACGGCGGCAGCAGAGACGGAAAC
  CCGCACGCACACGCTGACCCGAGTGAATCTGGAGAGCCCGGGGCCAGAGCGCGTATGGCGCGCTTCCTGG
  GGAGAGTTCCAGGCGCGTGTCCCGGAGCGCGCGCCCCTGCCGCCCCCGGCCCCCTCTCAATTCCAGGCGC
  GTATGCCCGACAGCGGGCCCCTTCCCGAAACCCACAAGTTCGGGGAAGGAGTGTCCTCCCCCAAAACACA
  CCTAGGCGAGGCATTGGCACCCCTGTCCAAGGCGTACCAAGGCGTGGCCGCCCCGTTCCCCAAGGCGCGC
  CGGCCGGAGAGCGCACTCCTGGGCGGCTCCGAGGCGGGCGAGCGCCTTCTCCAGCAAGGGCTGGCGCAGG
  TGGAGGCCGGGCGGCGGCAGGCGGAGGCCACGCGGCAGGCCGCGGCGCAGGAAGAGCGGCTGGCCGACCT
  CGCCTCGGACCTGCTGCTCCAGTATTTGCTGCAGGGCGGGGCCCGGCAGCGCGGCCTCGGGGGTCGGGGG
  CTGCAGGAGGCGGCGGAGGAGCGAGAGAGTGCAAGGGAGGAGGAGGAGGCGGAGCAGGAGAGACGCGGCG
  GGGAGGAGAGGGTGGGGGAAGAGGATGAGGAGGCGGCCGAGGCGGAGGCAGAGGCGGAGGAGGCGGAGAG
  GGCGCGGCAGAACGCGCTCCTGTTCGCGGAGGAGGAGGACGGGGAAGCCGGCGCCGAGGACAAGCGCTCC
  CAGGAGGAGACGCCGGGCCACCGGCGGAAGGAGGCCGAGGGGACAGAGGAGGGCGGGGAGGAGGAGGACG
  ACGAGGAGATGGATCCGCAGACGATCGACAGCCTCATTGAGCTGTCCACCAAACTCCACCTGCCAGCGGA
  CGACGTGGTCAGCATCATCGAGGAGGTGGAGGAGAAGCGGAAGCGGAAGAAGAACGCCCCTCCCGAGCCC
  GTGCCGCCCCCCCGTGCCGCCCCCGCCCCCACCCACGTCCGCTCCCCGCAGCCCCCGCCCCCCGCCCCCG
  CTCCCGCACGAGACGAGCTGCCGGACTGGAACGAGGTGCTCCCGCCCTGGGATCGGGAGGAGGACGAGGT
  GTACCCGCCAGGGCCGTACCACCCTTTCCCCAACTACATCCGGCCGCGGACACTGCAGCCGCCCTCGGCC
  TTGCGCCGCCGCCACTACCACCACGCCTTGCCGCCTTCGCGCCACTATCCCGGCCGGGAGGCCCAGGCGC
  GGCGCGCGCAGGAGGAGGCGGAGGCGGAGGAGCGCCGGCTGCAGGAGCAGGAGGAGCTGGAGAATTACAT
  CGAGCACGTGCTGCTCCGGCGCCCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209477 representing NM_003378
  Red=Cloning site Green=Tags(s)
  
  MKALRLSASALFCLLLINGLGAAPPGRPEAQPPPLSSEHKEPVAGDAVPGPKDGSAPEVRGARNSEPQDE
  GELFQGVDPRALAAVLLQALDRPASPPAPSGSQQGPEEEAAEALLTETVRSQTHSLPAPESPEPAAPPRP
  QTPENGPEASDPSEELEALASLLQELRDFSPSSAKRQQETAAAETETRTHTLTRVNLESPGPERVWRASW
  GEFQARVPERAPLPPPAPSQFQARMPDSGPLPETHKFGEGVSSPKTHLGEALAPLSKAYQGVAAPFPKAR
  RPESALLGGSEAGERLLQQGLAQVEAGRRQAEATRQAAAQEERLADLASDLLLQYLLQGGARQRGLGGRG
  LQEAAEERESAREEEEAEQERRGGEERVGEEDEEAAEAEAEAEEAERARQNALLFAEEEDGEAGAEDKRS
  QEETPGHRRKEAEGTEEGGEEEDDEEMDPQTIDSLIELSTKLHLPADDVVSIIEEVEEKRKRKKNAPPEP
  VPPPRAAPAPTHVRSPQPPPPAPAPARDELPDWNEVLPPWDREEDEVYPPGPYHPFPNYIRPRTLQPPSA
  LRRRHYHHALPPSRHYPGREAQARRAQEEAEAEERRLQEQEELENYIEHVLLRRP
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003378
• ORF Size: 1845 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003378.4
• RefSeq Size: 2545 bp
• RefSeq ORF: 1848 bp
• Locus ID: 7425
• UniProt ID: O15240
• Cytogenetics: 7q22.1
• Protein Families: Secreted Protein
• MW: 67.26 kDa
• Gene Summary: This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]