VGF (NM_003378) Human Tagged ORF Clone

CAT#: RC209477

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VGF (Myc-DDK-tagged)-Human VGF nerve growth factor inducible (VGF)



  "NM_003378" in other vectors (6)

Reconstitution Protocol

USD 572.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol VGF
Synonyms SCG7; SgVII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209477 representing NM_003378
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGCCCTCAGATTGTCGGCTTCCGCCCTCTTCTGCCTTCTGCTGATCAACGGGTTAGGGGCAGCAC
CCCCTGGTCGCCCTGAGGCGCAGCCTCCTCCTCTCAGCTCTGAGCATAAAGAGCCGGTAGCCGGGGACGC
AGTGCCCGGGCCAAAGGATGGCAGCGCCCCAGAGGTCCGAGGCGCTCGGAATTCCGAGCCGCAGGACGAG
GGAGAGCTTTTCCAGGGCGTGGATCCCCGGGCGCTGGCCGCGGTGCTGCTGCAGGCACTCGACCGTCCCG
CCTCACCCCCGGCACCAAGCGGCTCCCAGCAGGGGCCGGAGGAAGAAGCAGCTGAAGCTCTGCTGACCGA
GACCGTGCGCAGCCAGACCCACAGCCTCCCGGCGCCGGAGAGCCCGGAGCCCGCGGCTCCGCCTCGCCCT
CAGACTCCGGAGAATGGGCCCGAGGCGAGCGATCCCTCCGAGGAGCTCGAGGCGCTAGCGTCCCTGCTCC
AGGAACTGCGAGATTTCAGTCCAAGTAGCGCCAAGCGCCAGCAGGAGACGGCGGCAGCAGAGACGGAAAC
CCGCACGCACACGCTGACCCGAGTGAATCTGGAGAGCCCGGGGCCAGAGCGCGTATGGCGCGCTTCCTGG
GGAGAGTTCCAGGCGCGTGTCCCGGAGCGCGCGCCCCTGCCGCCCCCGGCCCCCTCTCAATTCCAGGCGC
GTATGCCCGACAGCGGGCCCCTTCCCGAAACCCACAAGTTCGGGGAAGGAGTGTCCTCCCCCAAAACACA
CCTAGGCGAGGCATTGGCACCCCTGTCCAAGGCGTACCAAGGCGTGGCCGCCCCGTTCCCCAAGGCGCGC
CGGCCGGAGAGCGCACTCCTGGGCGGCTCCGAGGCGGGCGAGCGCCTTCTCCAGCAAGGGCTGGCGCAGG
TGGAGGCCGGGCGGCGGCAGGCGGAGGCCACGCGGCAGGCCGCGGCGCAGGAAGAGCGGCTGGCCGACCT
CGCCTCGGACCTGCTGCTCCAGTATTTGCTGCAGGGCGGGGCCCGGCAGCGCGGCCTCGGGGGTCGGGGG
CTGCAGGAGGCGGCGGAGGAGCGAGAGAGTGCAAGGGAGGAGGAGGAGGCGGAGCAGGAGAGACGCGGCG
GGGAGGAGAGGGTGGGGGAAGAGGATGAGGAGGCGGCCGAGGCGGAGGCAGAGGCGGAGGAGGCGGAGAG
GGCGCGGCAGAACGCGCTCCTGTTCGCGGAGGAGGAGGACGGGGAAGCCGGCGCCGAGGACAAGCGCTCC
CAGGAGGAGACGCCGGGCCACCGGCGGAAGGAGGCCGAGGGGACAGAGGAGGGCGGGGAGGAGGAGGACG
ACGAGGAGATGGATCCGCAGACGATCGACAGCCTCATTGAGCTGTCCACCAAACTCCACCTGCCAGCGGA
CGACGTGGTCAGCATCATCGAGGAGGTGGAGGAGAAGCGGAAGCGGAAGAAGAACGCCCCTCCCGAGCCC
GTGCCGCCCCCCCGTGCCGCCCCCGCCCCCACCCACGTCCGCTCCCCGCAGCCCCCGCCCCCCGCCCCCG
CTCCCGCACGAGACGAGCTGCCGGACTGGAACGAGGTGCTCCCGCCCTGGGATCGGGAGGAGGACGAGGT
GTACCCGCCAGGGCCGTACCACCCTTTCCCCAACTACATCCGGCCGCGGACACTGCAGCCGCCCTCGGCC
TTGCGCCGCCGCCACTACCACCACGCCTTGCCGCCTTCGCGCCACTATCCCGGCCGGGAGGCCCAGGCGC
GGCGCGCGCAGGAGGAGGCGGAGGCGGAGGAGCGCCGGCTGCAGGAGCAGGAGGAGCTGGAGAATTACAT
CGAGCACGTGCTGCTCCGGCGCCCG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC209477 representing NM_003378
Red=Cloning site Green=Tags(s)

MKALRLSASALFCLLLINGLGAAPPGRPEAQPPPLSSEHKEPVAGDAVPGPKDGSAPEVRGARNSEPQDE
GELFQGVDPRALAAVLLQALDRPASPPAPSGSQQGPEEEAAEALLTETVRSQTHSLPAPESPEPAAPPRP
QTPENGPEASDPSEELEALASLLQELRDFSPSSAKRQQETAAAETETRTHTLTRVNLESPGPERVWRASW
GEFQARVPERAPLPPPAPSQFQARMPDSGPLPETHKFGEGVSSPKTHLGEALAPLSKAYQGVAAPFPKAR
RPESALLGGSEAGERLLQQGLAQVEAGRRQAEATRQAAAQEERLADLASDLLLQYLLQGGARQRGLGGRG
LQEAAEERESAREEEEAEQERRGGEERVGEEDEEAAEAEAEAEEAERARQNALLFAEEEDGEAGAEDKRS
QEETPGHRRKEAEGTEEGGEEEDDEEMDPQTIDSLIELSTKLHLPADDVVSIIEEVEEKRKRKKNAPPEP
VPPPRAAPAPTHVRSPQPPPPAPAPARDELPDWNEVLPPWDREEDEVYPPGPYHPFPNYIRPRTLQPPSA
LRRRHYHHALPPSRHYPGREAQARRAQEEAEAEERRLQEQEELENYIEHVLLRRP

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003378
ORF Size 1845 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003378.4
RefSeq Size 2545 bp
RefSeq ORF 1848 bp
Locus ID 7425
UniProt ID O15240
Cytogenetics 7q22.1
Protein Families Secreted Protein
MW 67.26 kDa
Gene Summary This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

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