DDX6 (NM_004397) Human Tagged ORF Clone

CAT#: RC209431

DDX6 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DDX6 (Cat# RC209431, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX6(Cat# TA804323). Positive lysates LY401399 (100ug) and LC401399 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401399) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209431 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DDX6 protein (Cat# TP309431). The protein was produced from HEK293T cells transfected with DDX6 cDNA clone (Cat# RC209431) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DDX6
• Synonyms: HLR2; IDDILF; P54; RCK; Rck/p54
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209431 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACGGCCAGAACAGAGAACCCTGTTATAATGGGTCTGTCCAGTCAAAATGGTCAGCTGAGAGGCC
  CTGTGAAACCCACTGGTGGCCCTGGAGGAGGGGGCACACAGACACAGCAACAGATGAACCAGCTGAAAAA
  CACCAACACAATCAATAATGGCACTCAGCAGCAAGCACAGAGTATGACCACCACTATTAAACCTGGTGAT
  GACTGGAAAAAGACTTTAAAACTCCCTCCAAAGGATCTAAGAATCAAAACTTCGGATGTGACCTCCACAA
  AAGGAAATGAGTTTGAAGATTACTGTTTGAAACGGGAGTTACTGATGGGAATTTTTGAAATGGGCTGGGA
  AAAGCCATCTCCTATTCAGGAGGAGAGCATTCCCATTGCTTTATCTGGTAGGGATATCTTAGCTAGAGCA
  AAAAATGGAACAGGCAAGAGCGGTGCCTACCTCATTCCCTTACTTGAACGGCTAGACCTGAAGAAGGACA
  ATATACAAGCAATGGTGATTGTTCCCACTAGAGAACTTGCTCTACAGGTCAGTCAAATTTGCATCCAGGT
  CAGCAAACACATGGGAGGGGCCAAAGTGATGGCAACCACAGGAGGAACCAATTTACGAGATGACATAATG
  AGGCTTGATGATACAGTGCACGTGGTGATTGCTACCCCTGGGAGAATCCTGGATCTTATTAAGAAAGGAG
  TAGCAAAGGTTGATCATGTCCAGATGATAGTATTGGATGAGGCAGATAAGTTGCTGTCACAGGATTTTGT
  GCAGATAATGGAGGATATTATTCTCACGCTACCTAAAAACAGGCAGATTTTACTATATTCCGCTACTTTC
  CCTCTTAGTGTACAGAAGTTCATGAATTCCCATTTGCAGAAACCCTATGAGATTAACCTGATGGAGGAAC
  TAACTCTGAAGGGAGTAACCCAGTACTACGCATATGTAACTGAGCGCCAAAAAGTACACTGCCTCAACAC
  ACTTTTCTCCAGGCTTCAGATAAACCAGTCGATCATTTTCTGTAACTCCTCTCAGCGAGTTGAATTGCTA
  GCCAAGAAGATTTCTCAACTGGGTTATTCTTGCTTCTATATTCATGCTAAAATGAGGCAGGAACATCGAA
  ATCGTGTATTTCATGATTTCCGAAATGGCTTATGCCGCAATCTTGTTTGCACTGATCTGTTTACCCGAGG
  TATTGATATACAAGCTGTGAATGTGGTAATAAACTTTGATTTCCCAAAGCTGGCAGAGACCTATCTCCAT
  CGTATTGGAAGATCAGGTCGCTTTGGTCATCTTGGCTTAGCCATCAACTTGATCACATATGATGATCGCT
  TCAACCTGAAAAGTATTGAGGAGCAGCTGGGAACAGAAATTAAACCTATTCCGAGCAACATTGATAAGAG
  CCTGTATGTGGCAGAATACCACAGCGAGCCTGTAGAAGATGAGAAACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209431 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGD
  DWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARA
  KNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIM
  RLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATF
  PLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELL
  AKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLH
  RIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004397
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004397.6
• RefSeq Size: 6246 bp
• RefSeq ORF: 1452 bp
• Locus ID: 1656
• UniProt ID: P26196
• Cytogenetics: 11q23.3
• Domains: DEAD, helicase_C
• Protein Pathways: RNA degradation
• MW: 54.4 kDa
• Gene Summary: This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012]