PRMT3 (NM_005788) Human Tagged ORF Clone

CAT#: RC209382

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PRMT3 (Myc-DDK-tagged)-Human protein arginine methyltransferase 3 (PRMT3), transcript variant 1



  "NM_005788" in other vectors (7)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 544.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PRMT3
Synonyms HRMT1L3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209382 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCTCGTTAGCGTCAGGCGCTACCGGCGGCCGGGGCGCTGTGGAGAATGAGGAGGACCTGCCAGAAC
TGTCGGACAGCGGGGACGAGGCCGCCTGGGAGGATGAGGACGATGCAGATCTCCCCCACGGCAAGCAGCA
GACCCCCTGCCTGTTCTGTAACAGGTTATTCACATCTGCTGAAGAAACATTTTCACACTGTAAGTCTGAG
CATCAGTTTAATATTGACAGCATGGTTCATAAACATGGACTTGAATTTTATGGATACATTAAGCTAATAA
ATTTTATTAGACTTAAGAATCCTACAGTTGAGTACATGAATTCCATATACAACCCAGTGCCTTGGGAGAA
AGAAGAGTATTTGAAGCCAGTATTAGAAGATGACCTTTTACTTCAATTTGATGTAGAAGATCTTTATGAA
CCGGTGTCAGTACCCTTCTCATACCCCAATGGACTCAGTGAAAATACATCTGTTGTTGAAAAATTGAAAC
ATATGGAAGCCAGGGCACTGTCTGCTGAAGCCGCATTGGCCAGAGCACGTGAGGATCTGCAAAAAATGAA
ACAATTTGCTCAGGATTTTGTGATGCACACAGATGTCAGAACCTGCTCGTCATCTACTAGTGTCATTGCG
GACCTCCAGGAGGATGAGGATGGTGTTTATTTCAGCTCATACGGGCATTATGGGATACATGAAGAAATGC
TAAAGGACAAAATACGAACAGAAAGCTACCGAGATTTCATATACCAAAATCCACATATCTTCAAAGACAA
GGTAGTTTTGGATGTTGGGTGTGGAACTGGAATTCTCTCTATGTTTGCTGCTAAAGCTGGGGCGAAGAAG
GTTCTTGGAGTTGATCAATCTGAAATACTTTACCAGGCAATGGATATTATAAGACTAAATAAACTTGAAG
ATACTATTACACTAATTAAAGGAAAGATTGAAGAAGTTCATCTTCCTGTAGAAAAAGTAGATGTTATCAT
ATCTGAGTGGATGGGCTATTTTCTTCTGTTTGAGTCTATGTTAGATTCTGTCCTTTATGCAAAGAACAAA
TACTTGGCAAAAGGAGGCTCGGTCTACCCTGACATTTGCACTATCAGCCTTGTAGCAGTGAGTGATGTGA
ATAAACATGCTGATAGAATTGCTTTTTGGGATGATGTCTATGGCTTCAAGATGTCCTGCATGAAGAAAGC
AGTTATTCCAGAAGCTGTTGTGGAAGTTTTAGATCCGAAGACTCTTATTTCAGAACCTTGTGGTATTAAG
CATATAGATTGCCATACGACGTCTATCTCAGATTTGGAATTTTCATCAGATTTTACCCTGAAAATCACAA
GGACATCCATGTGCACGGCAATTGCTGGCTACTTTGATATATATTTTGAGAAGAATTGCCACAACAGGGT
CGTGTTCTCTACGGGCCCTCAGAGCACCAAAACACACTGGAAACAAACAGTATTTCTACTGGAAAAACCA
TTTTCAGTTAAAGCAGGTGAAGCCTTGAAAGGAAAGGTCACAGTTCACAAGAATAAGAAAGATCCACGTT
CTCTCACCGTGACCCTCACGTTGAATAATTCAACTCAAACTTATGGTCTCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209382 protein sequence
Red=Cloning site Green=Tags(s)

MCSLASGATGGRGAVENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTPCLFCNRLFTSAEETFSHCKSE
HQFNIDSMVHKHGLEFYGYIKLINFIRLKNPTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYE
PVSVPFSYPNGLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVRTCSSSTSVIA
DLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKAGAKK
VLGVDQSEILYQAMDIIRLNKLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAKNK
YLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAVIPEAVVEVLDPKTLISEPCGIK
HIDCHTTSISDLEFSSDFTLKITRTSMCTAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKP
FSVKAGEALKGKVTVHKNKKDPRSLTVTLTLNNSTQTYGLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005788
ORF Size 1593 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005788.4
RefSeq Size 2743 bp
RefSeq ORF 1596 bp
Locus ID 10196
UniProt ID O60678
Cytogenetics 11p15.1
Protein Families Druggable Genome
MW 59.9 kDa
Gene Summary This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

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