HTR2B (NM_000867) Human Tagged ORF Clone

CAT#: RC209364

HTR2B (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 2B (HTR2B)

Product Images

Circular map for RC209364

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HTR2B
• Synonyms: 5-HT(2B); 5-HT-2B; 5-HT2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209364 representing NM_000867
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCTCTTACAGAGTGTCTGAACTTCAAAGCACAATTCCTGAGCACATTTTGCAGAGCACCTTTG
  TTCACGTTATCTCTTCTAACTGGTCTGGATTACAGACAGAATCAATACCAGAGGAAATGAAACAGATTGT
  TGAGGAACAGGGAAATAAACTGCACTGGGCAGCTCTTCTGATACTCATGGTGATAATACCCACAATTGGT
  GGAAATACCCTTGTTATTCTGGCTGTTTCACTGGAGAAGAAGCTGCAGTATGCTACTAATTACTTTCTAA
  TGTCCTTGGCGGTGGCTGATTTGCTGGTTGGATTGTTTGTGATGCCAATTGCCCTCTTGACAATAATGTT
  TGAGGCTATGTGGCCCCTCCCACTTGTTCTATGTCCTGCCTGGTTATTTCTTGACGTTCTCTTTTCAACC
  GCATCCATCATGCATCTCTGTGCCATTTCAGTGGATCGTTACATAGCCATCAAAAAGCCAATCCAGGCCA
  ATCAATATAACTCACGGGCTACAGCATTCATCAAGATTACAGTGGTGTGGTTAATTTCAATAGGCATTGC
  CATTCCAGTCCCTATTAAAGGGATAGAGACTGATGTGGACAACCCAAACAATATCACTTGTGTGCTGACA
  AAGGAACGTTTTGGCGATTTCATGCTCTTTGGCTCACTGGCTGCCTTCTTCACACCTCTTGCAATTATGA
  TTGTCACCTACTTTCTCACTATCCATGCTTTACAGAAGAAGGCTTACTTAGTCAAAAACAAGCCACCTCA
  ACGCCTAACATGGTTGACTGTGTCTACAGTTTTCCAAAGGGATGAAACACCTTGCTCGTCACCGGAAAAG
  GTGGCAATGCTGGATGGTTCTCGAAAGGACAAGGCTCTGCCCAACTCAGGTGATGAAACACTTATGCGAA
  GAACATCCACAATTGGGAAAAAGTCAGTGCAGACCATTTCCAACGAACAGAGAGCCTCAAAGGTCCTAGG
  GATTGTGTTTTTCCTCTTTTTGCTTATGTGGTGTCCCTTCTTTATTACAAATATAACTTTAGTTTTATGT
  GATTCCTGTAACCAAACTACTCTCCAAATGCTCCTGGAGATATTTGTGTGGATAGGCTATGTTTCCTCAG
  GAGTGAATCCTTTGGTCTACACCCTCTTCAATAAGACATTTCGGGATGCATTTGGCCGATATATCACCTG
  CAATTACCGGGCCACAAAGTCAGTAAAAACTCTCAGAAAACGCTCCAGTAAGATCTACTTCCGGAATCCA
  ATGGCAGAGAACTCTAAGTTTTTCAAGAAACATGGAATTCGAAATGGGATTAACCCTGCCATGTACCAGA
  GTCCAATGAGGCTCCGAAGTTCAACCATTCAGTCTTCATCAATCATTCTACTAGATACGCTTCTCCTCAC
  TGAAAATGAAGGTGACAAAACTGAAGAGCGAGTTAGTTATGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209364 representing NM_000867
  Red=Cloning site Green=Tags(s)
  
  MALSYRVSELQSTIPEHILQSTFVHVISSNWSGLQTESIPEEMKQIVEEQGNKLHWAALLILMVIIPTIG
  GNTLVILAVSLEKKLQYATNYFLMSLAVADLLVGLFVMPIALLTIMFEAMWPLPLVLCPAWLFLDVLFST
  ASIMHLCAISVDRYIAIKKPIQANQYNSRATAFIKITVVWLISIGIAIPVPIKGIETDVDNPNNITCVLT
  KERFGDFMLFGSLAAFFTPLAIMIVTYFLTIHALQKKAYLVKNKPPQRLTWLTVSTVFQRDETPCSSPEK
  VAMLDGSRKDKALPNSGDETLMRRTSTIGKKSVQTISNEQRASKVLGIVFFLFLLMWCPFFITNITLVLC
  DSCNQTTLQMLLEIFVWIGYVSSGVNPLVYTLFNKTFRDAFGRYITCNYRATKSVKTLRKRSSKIYFRNP
  MAENSKFFKKHGIRNGINPAMYQSPMRLRSSTIQSSSIILLDTLLLTENEGDKTEERVSYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000867
• ORF Size: 1443 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000867.3
• RefSeq Size: 2260 bp
• RefSeq ORF: 1446 bp
• Locus ID: 3357
• UniProt ID: P41595
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Gap junction, Neuroactive ligand-receptor interaction
• MW: 54.1 kDa
• Gene Summary: This gene encodes one of the several different receptors for 5-hydroxytryptamine (serotonin) that belongs to the G-protein coupled receptor 1 family. Serotonin is a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Serotonin receptors mediate many of the central and peripheral physiologic functions of serotonin, including regulation of cardiovascular functions and impulsive behavior. Population and family-based analyses of a minor allele (glutamine-to-stop substitution, designated Q20*) which blocks expression of this protein, and knockout studies in mice, suggest a role for this gene in impulsivity. However, other factors, such as elevated testosterone levels, may also be involved. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016]