Cullin 5 (CUL5) (NM_003478) Human Tagged ORF Clone

CAT#: RC209273

CUL5 (Myc-DDK-tagged)-Human cullin 5 (CUL5)

Product Images

Circular map for RC209273

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  Western blot validation of overexpression lysate (Cat# LY401176) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209273 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CUL5 protein (Cat# TP309273). The protein was produced from HEK293T cells transfected with CUL5 cDNA clone (Cat# RC209273) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cullin 5
• Synonyms: CUL-5; VACM-1; VACM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209273 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGTCTAATCTGTTAAAGAATAAAGGTTCTCTTCAGTTTGAAGACAAATGGGATTTTATGCGCC
  CGATTGTTTTGAAGCTTTTACGCCAGGAATCTGTTACAAAACAGCAGTGGTTTGATCTGTTTTCGGATGT
  GCATGCAGTCTGTCTTTGGGATGATAAAGGCCCAGCAAAAATTCATCAGGCTTTAAAAGAAGATATTCTT
  GAGTTTATTAAGCAGGCACAGGCACGAGTACTGAGCCATCAAGATGATACGGCTTTGCTAAAAGCATATA
  TTGTTGAATGGCGAAAGTTCTTTACACAATGTGATATTTTACCAAAACCTTTTTGTCAACTAGAGATTAC
  TTTAATGGGTAAACAGGGCAGCAATAAAAAATCAAATGTGGAAGACAGTATTGTTCGAAAGCTTATGCTT
  GATACATGGAATGAGTCAATCTTTTCAAACATAAAAAACAGACTCCAAGATAGTGCAATGAAGCTGGTAC
  ATGCTGAGAGATTGGGAGAAGCTTTTGATTCTCAGCTGGTTATTGGAGTAAGAGAATCCTATGTTAACCT
  TTGTTCTAATCCTGAGGATAAACTTCAAATTTATAGGGACAATTTTGAGAAGGCATACTTGGATTCAACA
  GAGAGATTTTATAGAACACAAGCACCCTCGTATTTACAACAAAATGGTGTACAGAATTATATGAAATATG
  CAGATGCTAAATTAAAAGAAGAAGAAAAACGAGCACTACGTTATTTAGAAACAAGACGAGAATGTAACTC
  CGTTGAAGCACTCATGGAATGCTGTGTAAATGCCCTGGTGACATCATTTAAAGAGACTATCTTAGCTGAG
  TGCCAAGGCATGATCAAGAGAAATGAAACTGAAAAATTACATTTAATGTTTTCATTGATGGACAAAGTTC
  CTAATGGTATAGAGCCAATGTTGAAAGACTTGGAGGAACATATCATTAGTGCTGGCCTGGCAGATATGGT
  AGCAGCTGCTGAAACTATTACTACTGACTCTGAGAAATACGTTGAGCAGTTACTTACACTATTTAATAGA
  TTTAGTAAACTCGTCAAAGAAGCTTTTCAAGATGATCCACGATTTCTTACTGCAAGAGATAAGGCGTATA
  AAGCAGTTGTTAATGATGCTACCATATTTAAACTTGAATTACCTTTGAAGCAGAAGGGGGTGGGATTAAA
  AACTCAGCCTGAATCAAAATGCCCTGAGCTGCTTGCCAATTACTGTGACATGTTGCTAAGAAAAACACCA
  TTAAGCAAAAAACTAACCTCTGAAGAGATTGAAGCAAAGCTTAAAGAAGTGCTCTTGGTACTTAAGTATG
  TACAGAACAAAGATGTTTTTATGAGGTATCATAAAGCTCATTTGACACGACGTCTTATATTAGACATCTC
  TGCCGATAGTGAAATTGAAGAAAACATGGTAGAGTGGCTAAGAGAAGTTGGTATGCCAGCGGATTATGTA
  AACAAGCTTGCTAGAATGTTTCAGGACATAAAAGTATCTGAAGATTTGAACCAAGCTTTTAAGGAAATGC
  ACAAAAATAATAAATTGGCATTACCAGCTGATTCAGTTAATATAAAAATTCTGAATGCTGGCGCCTGGTC
  AAGAAGTTCTGAGAAAGTCTTTGTCTCACTTCCTACTGAACTGGAGGACTTGATACCGGAAGTAGAAGAA
  TTCTACAAAAAAAATCATAGTGGTAGAAAATTACATTGGCATCATCTCATGTCAAATGGAATTATAACAT
  TTAAGAATGAAGTTGGTCAATATGATTTGGAGGTAACCACGTTTCAGCTCGCTGTATTGTTTGCATGGAA
  CCAAAGACCCAGAGAGAAAATCAGCTTTGAAAATCTTAAGCTTGCAACTGAACTCCCTGATGCTGAACTT
  AGGAGGACTTTATGGTCTTTAGTAGCTTTCCCAAAACTCAAACGGCAAGTTTTGTTGTATGAACCTCAAG
  TCAACTCACCCAAAGACTTTACAGAAGGTACCCTCTTCTCAGTGAACCAGGAGTTCAGTTTAATAAAAAA
  TGCAAAGGTTCAGAAAAGGGGTAAAATCAACTTGATTGGACGTTTGCAGCTCACTACAGAAAGGATGAGA
  GAAGAAGAGAATGAAGGAATAGTTCAACTACGAATACTAAGAACCCAGGAAGCTATCATACAAATAATGA
  AAATGAGAAAGAAAATTAGTAATGCTCAGCTGCAGACTGAATTAGTAGAAATTTTGAAAAACATGTTCTT
  GCCACAAAAGAAAATGATAAAAGAGCAAATAGAGTGGCTAATAGAGCACAAATACATCAGAAGAGATGAA
  TCTGATATCAACACTTTCATATATATGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209273 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDIL
  EFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLML
  DTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDST
  ERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAE
  CQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNR
  FSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTP
  LSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYV
  NKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEE
  FYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAEL
  RRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMR
  EEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDE
  SDINTFIYMA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003478
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003478.2
• RefSeq Size: 6408 bp
• RefSeq ORF: 2343 bp
• Locus ID: 8065
• UniProt ID: Q93034
• Cytogenetics: 11q22.3
• Domains: CULLIN
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 91 kDa
• Gene Summary: Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.[UniProtKB/Swiss-Prot Function]