Cullin 5 (CUL5) (NM_003478) Human Tagged ORF Clone

CAT#: RC209273

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CUL5 (Myc-DDK-tagged)-Human cullin 5 (CUL5)



  "NM_003478" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CUL5
Synonyms CUL-5; VACM-1; VACM1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209273 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACGTCTAATCTGTTAAAGAATAAAGGTTCTCTTCAGTTTGAAGACAAATGGGATTTTATGCGCC
CGATTGTTTTGAAGCTTTTACGCCAGGAATCTGTTACAAAACAGCAGTGGTTTGATCTGTTTTCGGATGT
GCATGCAGTCTGTCTTTGGGATGATAAAGGCCCAGCAAAAATTCATCAGGCTTTAAAAGAAGATATTCTT
GAGTTTATTAAGCAGGCACAGGCACGAGTACTGAGCCATCAAGATGATACGGCTTTGCTAAAAGCATATA
TTGTTGAATGGCGAAAGTTCTTTACACAATGTGATATTTTACCAAAACCTTTTTGTCAACTAGAGATTAC
TTTAATGGGTAAACAGGGCAGCAATAAAAAATCAAATGTGGAAGACAGTATTGTTCGAAAGCTTATGCTT
GATACATGGAATGAGTCAATCTTTTCAAACATAAAAAACAGACTCCAAGATAGTGCAATGAAGCTGGTAC
ATGCTGAGAGATTGGGAGAAGCTTTTGATTCTCAGCTGGTTATTGGAGTAAGAGAATCCTATGTTAACCT
TTGTTCTAATCCTGAGGATAAACTTCAAATTTATAGGGACAATTTTGAGAAGGCATACTTGGATTCAACA
GAGAGATTTTATAGAACACAAGCACCCTCGTATTTACAACAAAATGGTGTACAGAATTATATGAAATATG
CAGATGCTAAATTAAAAGAAGAAGAAAAACGAGCACTACGTTATTTAGAAACAAGACGAGAATGTAACTC
CGTTGAAGCACTCATGGAATGCTGTGTAAATGCCCTGGTGACATCATTTAAAGAGACTATCTTAGCTGAG
TGCCAAGGCATGATCAAGAGAAATGAAACTGAAAAATTACATTTAATGTTTTCATTGATGGACAAAGTTC
CTAATGGTATAGAGCCAATGTTGAAAGACTTGGAGGAACATATCATTAGTGCTGGCCTGGCAGATATGGT
AGCAGCTGCTGAAACTATTACTACTGACTCTGAGAAATACGTTGAGCAGTTACTTACACTATTTAATAGA
TTTAGTAAACTCGTCAAAGAAGCTTTTCAAGATGATCCACGATTTCTTACTGCAAGAGATAAGGCGTATA
AAGCAGTTGTTAATGATGCTACCATATTTAAACTTGAATTACCTTTGAAGCAGAAGGGGGTGGGATTAAA
AACTCAGCCTGAATCAAAATGCCCTGAGCTGCTTGCCAATTACTGTGACATGTTGCTAAGAAAAACACCA
TTAAGCAAAAAACTAACCTCTGAAGAGATTGAAGCAAAGCTTAAAGAAGTGCTCTTGGTACTTAAGTATG
TACAGAACAAAGATGTTTTTATGAGGTATCATAAAGCTCATTTGACACGACGTCTTATATTAGACATCTC
TGCCGATAGTGAAATTGAAGAAAACATGGTAGAGTGGCTAAGAGAAGTTGGTATGCCAGCGGATTATGTA
AACAAGCTTGCTAGAATGTTTCAGGACATAAAAGTATCTGAAGATTTGAACCAAGCTTTTAAGGAAATGC
ACAAAAATAATAAATTGGCATTACCAGCTGATTCAGTTAATATAAAAATTCTGAATGCTGGCGCCTGGTC
AAGAAGTTCTGAGAAAGTCTTTGTCTCACTTCCTACTGAACTGGAGGACTTGATACCGGAAGTAGAAGAA
TTCTACAAAAAAAATCATAGTGGTAGAAAATTACATTGGCATCATCTCATGTCAAATGGAATTATAACAT
TTAAGAATGAAGTTGGTCAATATGATTTGGAGGTAACCACGTTTCAGCTCGCTGTATTGTTTGCATGGAA
CCAAAGACCCAGAGAGAAAATCAGCTTTGAAAATCTTAAGCTTGCAACTGAACTCCCTGATGCTGAACTT
AGGAGGACTTTATGGTCTTTAGTAGCTTTCCCAAAACTCAAACGGCAAGTTTTGTTGTATGAACCTCAAG
TCAACTCACCCAAAGACTTTACAGAAGGTACCCTCTTCTCAGTGAACCAGGAGTTCAGTTTAATAAAAAA
TGCAAAGGTTCAGAAAAGGGGTAAAATCAACTTGATTGGACGTTTGCAGCTCACTACAGAAAGGATGAGA
GAAGAAGAGAATGAAGGAATAGTTCAACTACGAATACTAAGAACCCAGGAAGCTATCATACAAATAATGA
AAATGAGAAAGAAAATTAGTAATGCTCAGCTGCAGACTGAATTAGTAGAAATTTTGAAAAACATGTTCTT
GCCACAAAAGAAAATGATAAAAGAGCAAATAGAGTGGCTAATAGAGCACAAATACATCAGAAGAGATGAA
TCTGATATCAACACTTTCATATATATGGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209273 protein sequence
Red=Cloning site Green=Tags(s)

MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDIL
EFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLML
DTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDST
ERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAE
CQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNR
FSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTP
LSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYV
NKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEE
FYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAEL
RRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMR
EEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDE
SDINTFIYMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003478
ORF Size 2340 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003478.2
RefSeq Size 6408 bp
RefSeq ORF 2343 bp
Locus ID 8065
UniProt ID Q93034
Cytogenetics 11q22.3
Domains CULLIN
Protein Families Druggable Genome
Protein Pathways Ubiquitin mediated proteolysis
MW 91 kDa
Gene Summary Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.[UniProtKB/Swiss-Prot Function]

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