Natriuretic Peptide Receptor A (NPR1) (NM_000906) Human Tagged ORF Clone

CAT#: RC209267

NPR1 (Myc-DDK-tagged)-Human natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) (NPR1)

Product Images

Circular map for RC209267

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NPR1 (Cat# RC209267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPR1(Cat# TA805600). Positive lysates LY424461 (100ug) and LC424461 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY424461) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209267 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NPR1 protein (Cat# TP309267). The protein was produced from HEK293T cells transfected with NPR1 cDNA clone (Cat# RC209267) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Natriuretic Peptide Receptor A
• Synonyms: ANPa; ANPRA; GUC2A; GUCY2A; NPRA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209267 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGGCCCCGGCGCCCCGCTGGCTCCCGCCTGCGCCTGCTCCTGCTCCTGCTGCTGCCGCCGCTGC
  TGCTGCTGCTCCGGGGCAGCCACGCGGGCAACCTGACGGTAGCCGTGGTACTGCCGCTGGCCAATACCTC
  GTACCCCTGGTCGTGGGCGCGCGTGGGACCCGCCGTGGAGCTGGCCCTGGCCCAGGTGAAGGCGCGCCCC
  GACTTGCTGCCGGGCTGGACGGTCCGCACGGTGCTGGGCAGCAGCGAAAACGCGCTGGGCGTCTGCTCCG
  ACACCGCAGCGCCCCTGGCCGCGGTGGACCTCAAGTGGGAGCACAACCCCGCTGTGTTCCTGGGCCCCGG
  CTGCGTGTACGCCGCCGCCCCAGTGGGGCGCTTCACCGCGCACTGGCGGGTCCCGCTGCTGACCGCCGGC
  GCCCCGGCGCTGGGCTTCGGTGTCAAGGACGAGTATGCGCTGACCACCCGCGCGGGGCCCAGCTACGCCA
  AGCTGGGGGACTTCGTGGCGGCGCTGCACCGACGGCTGGGCTGGGAGCGCCAAGCGCTCATGCTCTACGC
  CTACCGGCCGGGTGACGAAGAGCACTGCTTCTTCCTCGTGGAGGGGCTGTTCATGCGGGTCCGCGACCGC
  CTCAATATTACGGTGGACCACCTGGAGTTCGCCGAGGACGACCTCAGCCACTACACCAGGCTGCTGCGGA
  CCATGCCGCGCAAAGGCCGAGTTATCTACATCTGCAGCTCCCCTGATGCCTTCAGAACCCTCATGCTCCT
  GGCCCTGGAAGCTGGCTTGTGTGGGGAGGACTACGTTTTCTTCCACCTGGATATCTTTGGGCAAAGCCTG
  CAAGGTGGACAGGGCCCTGCTCCCCGCAGGCCCTGGGAGAGAGGGGATGGGCAGGATGTCAGTGCCCGCC
  AGGCCTTTCAGGCTGCCAAAATCATTACATATAAAGACCCAGATAATCCCGAGTACTTGGAATTCCTGAA
  GCAGTTAAAACACCTGGCCTATGAGCAGTTCAACTTCACCATGGAGGATGTCCTGGTGAACACCATCCCA
  GCATCCTTCCACGACGGGCTCCTGCTCTATATCCAGGCAGTGACGGAGACTCTGGCACATGGGGGAACTG
  TTACTGATGGGGAGAACATCACTCAGCGGATGTGGAACCGAAGCTTTCAAGGTGTGACAGGATACCTGAA
  AATTGATAGCAGTGGCGATCGGGAAACAGACTTCTCCCTCTGGGATATGGATCCCGAGAATGGTGCCTTC
  AGGGTTGTACTGAACTACAATGGGACTTCCCAAGAGCTGGTGGCTGTGTCGGGGCGCAAACTGAACTGGC
  CCCTGGGGTACCCTCCTCCTGACATCCCCAAATGTGGCTTTGACAACGAAGACCCAGCATGCAACCAAGA
  TCACCTTTCCACCCTGGAGGTGCTGGCTTTGGTGGGCAGCCTCTCCTTGCTCGGCATTCTGATTGTCTCC
  TTCTTCATATACAGGAAGATGCAGCTGGAGAAGGAACTGGCCTCGGAGCTGTGGCGGGTGCGCTGGGAGG
  ACGTTGAGCCCAGTAGCCTTGAGAGGCACCTGCGGAGTGCAGGCAGCCGGCTGACCCTGAGCGGGAGAGG
  CTCCAATTACGGCTCCCTGCTAACCACAGAGGGCCAGTTCCAAGTCTTTGCCAAGACAGCATATTATAAG
  GGCAACCTCGTGGCTGTGAAACGTGTGAACCGTAAACGCATTGAGCTGACACGAAAAGTCCTGTTTGAAC
  TGAAGCATATGCGGGATGTGCAGAATGAACACCTGACCAGGTTTGTGGGAGCCTGCACCGACCCCCCCAA
  TATCTGCATCCTCACAGAGTACTGTCCCCGTGGGAGCCTGCAGGACATTCTGGAGAATGAGAGCATCACC
  CTGGACTGGATGTTCCGGTACTCACTCACCAATGACATCGTCAAGGGCATGCTGTTTCTACACAATGGGG
  CTATCTGTTCCCATGGGAACCTCAAGTCATCCAACTGCGTGGTAGATGGGCGCTTTGTGCTCAAGATCAC
  CGACTATGGGCTGGAGAGCTTCAGGGACCTGGACCCAGAGCAAGGACACACCGTTTATGCCAAAAAGCTG
  TGGACGGCCCCTGAGCTCCTGCGAATGGCTTCACCCCCTGTGCGGGGCTCCCAGGCTGGTGACGTATACA
  GCTTTGGGATCATCCTTCAGGAGATTGCCCTGAGGAGTGGGGTCTTCCACGTGGAAGGTTTGGACCTGAG
  CCCCAAAGAGATCATCGAGCGGGTGACTCGGGGTGAGCAGCCCCCCTTCCGGCCCTCCCTGGCCCTGCAG
  AGTCACCTGGAGGAGTTGGGGCTGCTCATGCAGCGGTGCTGGGCTGAGGACCCACAGGAGAGGCCACCAT
  TCCAGCAGATCCGCCTGACGTTGCGCAAATTTAACAGGGAGAACAGCAGCAACATCCTGGACAACCTGCT
  GTCCCGCATGGAGCAGTACGCGAACAATCTGGAGGAACTGGTGGAGGAGCGGACCCAGGCATACCTGGAG
  GAGAAGCGCAAGGCTGAGGCCCTGCTCTACCAGATCCTGCCTCACTCAGTGGCTGAGCAGCTGAAGCGTG
  GGGAGACGGTGCAGGCCGAAGCCTTTGACAGTGTTACCATCTACTTCAGTGACATTGTGGGTTTCACAGC
  GCTGTCGGCGGAGAGCACGCCCATGCAGGTGGTGACCCTGCTCAATGACCTGTACACTTGCTTTGATGCT
  GTCATAGACAACTTTGATGTGTACAAGGTGGAGACAATTGGCGATGCCTACATGGTGGTGTCAGGGCTCC
  CTGTGCGGAACGGGCGGCTACACGCCTGCGAGGTAGCCCGCATGGCCCTGGCACTGCTGGATGCTGTGCG
  CTCCTTCCGAATCCGCCACCGGCCCCAGGAGCAGCTGCGCTTGCGCATTGGCATCCACACAGGACCTGTG
  TGTGCTGGAGTGGTGGGACTGAAGATGCCCCGTTACTGTCTCTTTGGGGATACAGTCAACACAGCCTCAA
  GAATGGAGTCTAATGGGGAAGCCCTGAAGATCCACTTGTCTTCTGAGACCAAGGCTGTCCTGGAGGAGTT
  TGGTGGTTTCGAGCTGGAGCTTCGAGGGGATGTAGAAATGAAGGGCAAAGGCAAGGTTCGGACCTACTGG
  CTCCTTGGGGAGAGGGGGAGTAGCACCCGAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209267 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPGPRRPAGSRLRLLLLLLLPPLLLLLRGSHAGNLTVAVVLPLANTSYPWSWARVGPAVELALAQVKARP
  DLLPGWTVRTVLGSSENALGVCSDTAAPLAAVDLKWEHNPAVFLGPGCVYAAAPVGRFTAHWRVPLLTAG
  APALGFGVKDEYALTTRAGPSYAKLGDFVAALHRRLGWERQALMLYAYRPGDEEHCFFLVEGLFMRVRDR
  LNITVDHLEFAEDDLSHYTRLLRTMPRKGRVIYICSSPDAFRTLMLLALEAGLCGEDYVFFHLDIFGQSL
  QGGQGPAPRRPWERGDGQDVSARQAFQAAKIITYKDPDNPEYLEFLKQLKHLAYEQFNFTMEDVLVNTIP
  ASFHDGLLLYIQAVTETLAHGGTVTDGENITQRMWNRSFQGVTGYLKIDSSGDRETDFSLWDMDPENGAF
  RVVLNYNGTSQELVAVSGRKLNWPLGYPPPDIPKCGFDNEDPACNQDHLSTLEVLALVGSLSLLGILIVS
  FFIYRKMQLEKELASELWRVRWEDVEPSSLERHLRSAGSRLTLSGRGSNYGSLLTTEGQFQVFAKTAYYK
  GNLVAVKRVNRKRIELTRKVLFELKHMRDVQNEHLTRFVGACTDPPNICILTEYCPRGSLQDILENESIT
  LDWMFRYSLTNDIVKGMLFLHNGAICSHGNLKSSNCVVDGRFVLKITDYGLESFRDLDPEQGHTVYAKKL
  WTAPELLRMASPPVRGSQAGDVYSFGIILQEIALRSGVFHVEGLDLSPKEIIERVTRGEQPPFRPSLALQ
  SHLEELGLLMQRCWAEDPQERPPFQQIRLTLRKFNRENSSNILDNLLSRMEQYANNLEELVEERTQAYLE
  EKRKAEALLYQILPHSVAEQLKRGETVQAEAFDSVTIYFSDIVGFTALSAESTPMQVVTLLNDLYTCFDA
  VIDNFDVYKVETIGDAYMVVSGLPVRNGRLHACEVARMALALLDAVRSFRIRHRPQEQLRLRIGIHTGPV
  CAGVVGLKMPRYCLFGDTVNTASRMESNGEALKIHLSSETKAVLEEFGGFELELRGDVEMKGKGKVRTYW
  LLGERGSSTRG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000906
• ORF Size: 3183 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000906.2, NP_000897.2
• RefSeq Size: 4201 bp
• RefSeq ORF: 3186 bp
• Locus ID: 4881
• UniProt ID: P16066
• Cytogenetics: 1q21.3
• Domains: pkinase, CYCc, ANF_receptor
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Purine metabolism, Vascular smooth muscle contraction
• MW: 119 kDa
• Gene Summary: Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM, May 2009]