HS3ST1 (NM_005114) Human Tagged ORF Clone

CAT#: RC209256

HS3ST1 (Myc-DDK-tagged)-Human heparan sulfate (glucosamine) 3-O-sulfotransferase 1 (HS3ST1)

Product Images

Circular map for RC209256

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  Western blot validation of overexpression lysate (Cat# LY417504) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209256 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HS3ST1 protein (Cat# TP309256). The protein was produced from HEK293T cells transfected with HS3ST1 cDNA clone (Cat# RC209256) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HS3ST1
• Synonyms: 3OST; 3OST1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209256 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGCTGCTCCTGGGCGCGGTGCTGCTGGTGGCCCAGCCCCAGCTAGTGCCTTCCCGCACCGCCG
  AGCTAGGCCAGCAGGAGCTTCTGCGGAAAGCGGGGACCCTCCAGGATGACGTCCGCGATGGCGTGGCCCC
  AAACGGCTCTGCCCAGCAGTTGCCGCAGACCATCATCATCGGCGTGCGCAAGGGCGGCACGCGCGCACTG
  CTGGAGATGCTCAGCCTGCACCCCGACGTGGCGGCCGCGGAGAACGAGGTCCACTTCTTCGACTGGGAGG
  AGCATTACAGCCACGGCTTGGGCTGGTACCTCAGCCAGATGCCCTTCTCCTGGCCACACCAGCTCACAGT
  GGAGAAGACCCCCGCGTATTTCACGTCGCCCAAAGTGCCTGAGCGAGTCTACAGCATGAACCCGTCCATC
  CGGCTGCTGCTCATCCTGCGAGACCCGTCGGAGCGCGTGCTATCTGACTACACCCAAGTGTTCTACAACC
  ACATGCAGAAGCACAAGCCCTACCCGTCCATCGAGGAGTTCCTGGTGCGCGATGGCAGGCTCAATGTGGA
  CTACAAGGCCCTCAACCGCAGCCTCTACCACGTGCACATGCAGAACTGGCTGCGCTTTTTCCCGCTGCGC
  CACATCCACATTGTGGACGGCGACCGCCTCATCAGGGACCCCTTCCCTGAGATCCAAAAGGTCGAGAGGT
  TCCTAAAGCTGTCGCCGCAGATCAATGCTTCGAACTTCTACTTTAACAAAACCAAGGGCTTTTACTGCCT
  GCGGGACAGCGGCCGGGACCGCTGCTTACATGAGTCCAAAGGCCGGGCGCACCCCCAAGTCGATCCCAAA
  CTACTCAATAAACTGCACGAATATTTTCATGAGCCAAATAAGAAGTTCTTCGAGCTTGTTGGCAGAACAT
  TTGACTGGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209256 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALLLGAVLLVAQPQLVPSRTAELGQQELLRKAGTLQDDVRDGVAPNGSAQQLPQTIIIGVRKGGTRAL
  LEMLSLHPDVAAAENEVHFFDWEEHYSHGLGWYLSQMPFSWPHQLTVEKTPAYFTSPKVPERVYSMNPSI
  RLLLILRDPSERVLSDYTQVFYNHMQKHKPYPSIEEFLVRDGRLNVDYKALNRSLYHVHMQNWLRFFPLR
  HIHIVDGDRLIRDPFPEIQKVERFLKLSPQINASNFYFNKTKGFYCLRDSGRDRCLHESKGRAHPQVDPK
  LLNKLHEYFHEPNKKFFELVGRTFDWH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005114
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005114.4
• RefSeq Size: 1965 bp
• RefSeq ORF: 924 bp
• Locus ID: 9957
• UniProt ID: O14792
• Cytogenetics: 4p15.33
• Domains: Sulfotransfer
• Protein Families: Druggable Genome
• Protein Pathways: Heparan sulfate biosynthesis
• MW: 35.8 kDa
• Gene Summary: Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity, anticoagulant heparan sulfate conversion activity, and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein. [provided by RefSeq, Jul 2008]