PP4R1 (PPP4R1) (NM_005134) Human Tagged ORF Clone

CAT#: RC209255

PPP4R1 (Myc-DDK-tagged)-Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 2

Product Images

Circular map for RC209255

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PPP4R1 (Cat# RC209255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP4R1 rabbit polyclonal antibody (Cat# TA890169).

• 

  Western blot validation of overexpression lysate (Cat# LY417496) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209255 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PPP4R1 protein (Cat# TP309255). The protein was produced from HEK293T cells transfected with PPP4R1 cDNA clone (Cat# RC209255) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PP4R1
• Synonyms: MEG1; PP4(Rmeg); PP4R1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209255 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCTCTCGCTGCTTCAGGAGGACCTGCAGGAGGACGCAGACGGATCCCTGGACTTTGTCTCAC
  AAGATGAAATGTTGACGCCCCTGGGGAGATTGGACAAGTATGCTGCAAGTGAGAACATATTTAACAGACA
  AATGGTGGCCCGGAGTTTGCTCGATACCTTGAGGGAAGTCTGCGATGATGAAAGAGATTGTATTGCTGTT
  TTGGAAAGAATTAGCAGATTGGCCGATGATTCAGAACCAACTGTGAGAGCGGAGCTGATGGAACAGGTGC
  CTCACATCGCACTGTTTTGTCAAGAAAACCGGCCTTCAATACCATATGCTTTTTCAAAATTCTTACTACC
  TATTGTGGTTAGATACCTTGCAGATCAGAATAATCAGGTGAGGAAAACAAGTCAGGCAGCTTTGCTGGCT
  CTGTTGGAGCAGGAGCTCATTGAACGATTTGATGTGGAGACCAAAGTGTGCCCTGTCCTCATAGAGCTGA
  CAGCCCCAGATAGCAATGATGATGTGAAAACAGAAGCTGTGGCTATAATGTGCAAAATGGCTCCCATGGT
  TGGGAAGGATATTACAGAGCGTCTTATCCTCCCTAGGTTTTGTGAGATGTGCTGCGATTGCAGAATGTTT
  CACGTTCGAAAGGTCTGTGCTGCCAATTTTGGAGATATTTGCAGTGTAGTTGGCCAGCAAGCTACTGAAG
  AAATGTTGCTGCCCAGATTTTTCCAGCTTTGTTCTGATAATGTATGGGGAGTCCGAAAGGCTTGTGCTGA
  ATGCTTCATGGCGGTTTCATGTGCAACATGTCAAGAAATCCGACGGACCAAATTATCAGCACTTTTTATT
  AATTTGATCAGTGATCCTTCACGTTGGGTTCGCCAAGCAGCTTTTCAGTCTCTGGGACCTTTCATATCTA
  CTTTTGCTAATCCATCTAGCTCAGGCCAGTATTTTAAAGAAGAAAGCAAAAGTTCAGAAGAGATGTCAGT
  AGAAAACAAAAATAGGACCAGAGATCAAGAAGCCCCAGAGGATGTACAAGTCAGGCCAGAGGATACTCCT
  TCAGATCTCAGTGTTAGTAATTCCAGTGTCATACTGGAAAACACGATGGAAGACCATGCTGCTGAGGCAT
  CCGGGAAGCCTCTAGGTGAAATTAGTGTTCCACTGGACAGCTCTTTACTTTGTACTTTGTCCTCAGAATC
  TCACCAGGAAGCAGCTAGTAATGAGAATGATAAAAAACCTGGTAACTACAAATCTATGTTACGACCAGAG
  GTTGGCACCACTTCACAAGATTCAGCTCTCTTAGATCAGGAATTGTATAACTCCTTCCATTTCTGGAGGA
  CTCCTCTTCCTGAAATAGATCTAGACATAGAGCTTGAACAGAACTCTGGGGGAAAACCCAGCCCAGAGGG
  ACCAGAGGAAGAATCTGAGGGCCCTGTGCCCAGTTCTCCAAACATCACCATGGCCACCAGAAAGGAACTG
  GAAGAAATGATAGAAAATCTAGAGCCCCACATTGATGATCCAGATGTTAAAGCACAAGTGGAAGTGCTGT
  CCGCTGCACTACGTGCTTCCAGCCTGGATGCACATGAAGAGACCATCAGTATAGAAAAGAGAAGTGATTT
  GCAAGATGAACTGGATATAAATGAGCTACCAAATTGTAAAATAAATCAAGAAGATTCTGTGCCTTTAATC
  AGCGATGCTGTTGAGAATATGGACTCCACTCTTCACTATATTCACAGCGATTCAGACTTGAGCAACAATA
  GCAGTTTTAGCCCTGATGAGGAAAGGAGAACTAAAGTACAAGATGTTGTACCTCAGGCGTTGTTAGATCA
  GTATTTATCTATGACTGACCCTTCTCGTGCACAGACGGTTGACACTGAAATTGCTAAGCACTGTGCATAT
  AGCCTCCCTGGTGTGGCCTTGACACTCGGAAGACAGAATTGGCACTGCCTGAGAGAGACGTATGAGACTC
  TGGCCTCAGACATGCAGTGGAAAGTTCGACGAACTCTAGCATTCTCCATCCACGAGCTTGCAGTTATTCT
  TGGAGATCAATTGACAGCTGCAGATCTGGTTCCAATTTTTAATGGATTTTTAAAAGACCTCGATGAAGTC
  AGGATAGGTGTTCTTAAACACTTGCATGATTTTCTGAAGCTTCTTCATATTGACAAAAGAAGAGAATATC
  TTTATCAACTTCAGGAGTTTTTGGTGACAGATAATAGTAGAAATTGGCGGTTTCGAGCTGAACTGGCTGA
  ACAGCTGATTTTACTTCTAGAGTTATATAGTCCCAGAGATGTTTATGACTATTTACGTCCCATTGCTCTG
  AATCTGTGTGCAGACAAAGTTTCTTCTGTTCGTTGGATTTCCTACAAGTTGGTCAGCGAGATGGTGAAGA
  AGCTGCACGCGGCAACACCACCAACGTTCGGAGTGGACCTCATCAATGAGCTTGTGGAGAACTTTGGCAG
  ATGTCCCAAGTGGTCTGGTCGGCAAGCCTTTGTCTTTGTCTGCCAGACTGTCATTGAGGATGACTGCCTT
  CCCATGGACCAGTTTGCTGTGCATCTCATGCCGCATCTGCTAACCTTAGCAAATGACAGGGTTCCTAACG
  TGCGAGTGCTGCTTGCAAAGACATTAAGACAAACTCTACTAGAAAAAGACTATTTCTTGGCCTCTGCCAG
  CTGCCACCAGGAGGCTGTGGAGCAGACCATCATGGCTCTTCAGATGGACCGTGACAGCGATGTCAAGTAT
  TTTGCAAGCATCCACCCTGCCAGTACCAAAATCTCCGAAGATGCCATGAGCACAGCGTCCTCAACCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209255 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADLSLLQEDLQEDADGSLDFVSQDEMLTPLGRLDKYAASENIFNRQMVARSLLDTLREVCDDERDCIAV
  LERISRLADDSEPTVRAELMEQVPHIALFCQENRPSIPYAFSKFLLPIVVRYLADQNNQVRKTSQAALLA
  LLEQELIERFDVETKVCPVLIELTAPDSNDDVKTEAVAIMCKMAPMVGKDITERLILPRFCEMCCDCRMF
  HVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVSCATCQEIRRTKLSALFI
  NLISDPSRWVRQAAFQSLGPFISTFANPSSSGQYFKEESKSSEEMSVENKNRTRDQEAPEDVQVRPEDTP
  SDLSVSNSSVILENTMEDHAAEASGKPLGEISVPLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPE
  VGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESEGPVPSSPNITMATRKEL
  EEMIENLEPHIDDPDVKAQVEVLSAALRASSLDAHEETISIEKRSDLQDELDINELPNCKINQEDSVPLI
  SDAVENMDSTLHYIHSDSDLSNNSSFSPDEERRTKVQDVVPQALLDQYLSMTDPSRAQTVDTEIAKHCAY
  SLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLTAADLVPIFNGFLKDLDEV
  RIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILLLELYSPRDVYDYLRPIAL
  NLCADKVSSVRWISYKLVSEMVKKLHAATPPTFGVDLINELVENFGRCPKWSGRQAFVFVCQTVIEDDCL
  PMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKDYFLASASCHQEAVEQTIMALQMDRDSDVKY
  FASIHPASTKISEDAMSTASSTY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005134
• ORF Size: 2799 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005134.4
• RefSeq Size: 3929 bp
• RefSeq ORF: 2802 bp
• Locus ID: 9989
• UniProt ID: Q8TF05
• Cytogenetics: 18p11.22
• Domains: HEAT
• Protein Families: Druggable Genome, Phosphatase
• MW: 105.2 kDa
• Gene Summary: This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]