PP4R1 (PPP4R1) (NM_005134) Human Tagged ORF Clone

CAT#: RC209255

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PPP4R1 (Myc-DDK-tagged)-Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 2



Reconstitution Protocol

USD 854.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PPP4R1
Synonyms MEG1; PP4(Rmeg); PP4R1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209255 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACCTCTCGCTGCTTCAGGAGGACCTGCAGGAGGACGCAGACGGATCCCTGGACTTTGTCTCAC
AAGATGAAATGTTGACGCCCCTGGGGAGATTGGACAAGTATGCTGCAAGTGAGAACATATTTAACAGACA
AATGGTGGCCCGGAGTTTGCTCGATACCTTGAGGGAAGTCTGCGATGATGAAAGAGATTGTATTGCTGTT
TTGGAAAGAATTAGCAGATTGGCCGATGATTCAGAACCAACTGTGAGAGCGGAGCTGATGGAACAGGTGC
CTCACATCGCACTGTTTTGTCAAGAAAACCGGCCTTCAATACCATATGCTTTTTCAAAATTCTTACTACC
TATTGTGGTTAGATACCTTGCAGATCAGAATAATCAGGTGAGGAAAACAAGTCAGGCAGCTTTGCTGGCT
CTGTTGGAGCAGGAGCTCATTGAACGATTTGATGTGGAGACCAAAGTGTGCCCTGTCCTCATAGAGCTGA
CAGCCCCAGATAGCAATGATGATGTGAAAACAGAAGCTGTGGCTATAATGTGCAAAATGGCTCCCATGGT
TGGGAAGGATATTACAGAGCGTCTTATCCTCCCTAGGTTTTGTGAGATGTGCTGCGATTGCAGAATGTTT
CACGTTCGAAAGGTCTGTGCTGCCAATTTTGGAGATATTTGCAGTGTAGTTGGCCAGCAAGCTACTGAAG
AAATGTTGCTGCCCAGATTTTTCCAGCTTTGTTCTGATAATGTATGGGGAGTCCGAAAGGCTTGTGCTGA
ATGCTTCATGGCGGTTTCATGTGCAACATGTCAAGAAATCCGACGGACCAAATTATCAGCACTTTTTATT
AATTTGATCAGTGATCCTTCACGTTGGGTTCGCCAAGCAGCTTTTCAGTCTCTGGGACCTTTCATATCTA
CTTTTGCTAATCCATCTAGCTCAGGCCAGTATTTTAAAGAAGAAAGCAAAAGTTCAGAAGAGATGTCAGT
AGAAAACAAAAATAGGACCAGAGATCAAGAAGCCCCAGAGGATGTACAAGTCAGGCCAGAGGATACTCCT
TCAGATCTCAGTGTTAGTAATTCCAGTGTCATACTGGAAAACACGATGGAAGACCATGCTGCTGAGGCAT
CCGGGAAGCCTCTAGGTGAAATTAGTGTTCCACTGGACAGCTCTTTACTTTGTACTTTGTCCTCAGAATC
TCACCAGGAAGCAGCTAGTAATGAGAATGATAAAAAACCTGGTAACTACAAATCTATGTTACGACCAGAG
GTTGGCACCACTTCACAAGATTCAGCTCTCTTAGATCAGGAATTGTATAACTCCTTCCATTTCTGGAGGA
CTCCTCTTCCTGAAATAGATCTAGACATAGAGCTTGAACAGAACTCTGGGGGAAAACCCAGCCCAGAGGG
ACCAGAGGAAGAATCTGAGGGCCCTGTGCCCAGTTCTCCAAACATCACCATGGCCACCAGAAAGGAACTG
GAAGAAATGATAGAAAATCTAGAGCCCCACATTGATGATCCAGATGTTAAAGCACAAGTGGAAGTGCTGT
CCGCTGCACTACGTGCTTCCAGCCTGGATGCACATGAAGAGACCATCAGTATAGAAAAGAGAAGTGATTT
GCAAGATGAACTGGATATAAATGAGCTACCAAATTGTAAAATAAATCAAGAAGATTCTGTGCCTTTAATC
AGCGATGCTGTTGAGAATATGGACTCCACTCTTCACTATATTCACAGCGATTCAGACTTGAGCAACAATA
GCAGTTTTAGCCCTGATGAGGAAAGGAGAACTAAAGTACAAGATGTTGTACCTCAGGCGTTGTTAGATCA
GTATTTATCTATGACTGACCCTTCTCGTGCACAGACGGTTGACACTGAAATTGCTAAGCACTGTGCATAT
AGCCTCCCTGGTGTGGCCTTGACACTCGGAAGACAGAATTGGCACTGCCTGAGAGAGACGTATGAGACTC
TGGCCTCAGACATGCAGTGGAAAGTTCGACGAACTCTAGCATTCTCCATCCACGAGCTTGCAGTTATTCT
TGGAGATCAATTGACAGCTGCAGATCTGGTTCCAATTTTTAATGGATTTTTAAAAGACCTCGATGAAGTC
AGGATAGGTGTTCTTAAACACTTGCATGATTTTCTGAAGCTTCTTCATATTGACAAAAGAAGAGAATATC
TTTATCAACTTCAGGAGTTTTTGGTGACAGATAATAGTAGAAATTGGCGGTTTCGAGCTGAACTGGCTGA
ACAGCTGATTTTACTTCTAGAGTTATATAGTCCCAGAGATGTTTATGACTATTTACGTCCCATTGCTCTG
AATCTGTGTGCAGACAAAGTTTCTTCTGTTCGTTGGATTTCCTACAAGTTGGTCAGCGAGATGGTGAAGA
AGCTGCACGCGGCAACACCACCAACGTTCGGAGTGGACCTCATCAATGAGCTTGTGGAGAACTTTGGCAG
ATGTCCCAAGTGGTCTGGTCGGCAAGCCTTTGTCTTTGTCTGCCAGACTGTCATTGAGGATGACTGCCTT
CCCATGGACCAGTTTGCTGTGCATCTCATGCCGCATCTGCTAACCTTAGCAAATGACAGGGTTCCTAACG
TGCGAGTGCTGCTTGCAAAGACATTAAGACAAACTCTACTAGAAAAAGACTATTTCTTGGCCTCTGCCAG
CTGCCACCAGGAGGCTGTGGAGCAGACCATCATGGCTCTTCAGATGGACCGTGACAGCGATGTCAAGTAT
TTTGCAAGCATCCACCCTGCCAGTACCAAAATCTCCGAAGATGCCATGAGCACAGCGTCCTCAACCTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209255 protein sequence
Red=Cloning site Green=Tags(s)

MADLSLLQEDLQEDADGSLDFVSQDEMLTPLGRLDKYAASENIFNRQMVARSLLDTLREVCDDERDCIAV
LERISRLADDSEPTVRAELMEQVPHIALFCQENRPSIPYAFSKFLLPIVVRYLADQNNQVRKTSQAALLA
LLEQELIERFDVETKVCPVLIELTAPDSNDDVKTEAVAIMCKMAPMVGKDITERLILPRFCEMCCDCRMF
HVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVSCATCQEIRRTKLSALFI
NLISDPSRWVRQAAFQSLGPFISTFANPSSSGQYFKEESKSSEEMSVENKNRTRDQEAPEDVQVRPEDTP
SDLSVSNSSVILENTMEDHAAEASGKPLGEISVPLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPE
VGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESEGPVPSSPNITMATRKEL
EEMIENLEPHIDDPDVKAQVEVLSAALRASSLDAHEETISIEKRSDLQDELDINELPNCKINQEDSVPLI
SDAVENMDSTLHYIHSDSDLSNNSSFSPDEERRTKVQDVVPQALLDQYLSMTDPSRAQTVDTEIAKHCAY
SLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLTAADLVPIFNGFLKDLDEV
RIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILLLELYSPRDVYDYLRPIAL
NLCADKVSSVRWISYKLVSEMVKKLHAATPPTFGVDLINELVENFGRCPKWSGRQAFVFVCQTVIEDDCL
PMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKDYFLASASCHQEAVEQTIMALQMDRDSDVKY
FASIHPASTKISEDAMSTASSTY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005134
ORF Size 2799 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005134.4
RefSeq Size 3929 bp
RefSeq ORF 2802 bp
Locus ID 9989
UniProt ID Q8TF05
Cytogenetics 18p11.22
Domains HEAT
Protein Families Druggable Genome, Phosphatase
MW 105.2 kDa
Gene Summary This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
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