Eph receptor A3 (EPHA3) (NM_005233) Human Tagged ORF Clone

CAT#: RC209191

EPHA3 (Myc-DDK-tagged)-Human EPH receptor A3 (EPHA3), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EPHA3 (Cat# RC209191, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHA3 (Cat# TA890116). Positive lysates LY417428 (100ug) and LC417428 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY417428) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209191 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EPHA3 protein (Cat# TP309191). The protein was produced from HEK293T cells transfected with EPHA3 cDNA clone (Cat# RC209191) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EPHA3
• Synonyms: EK4; ETK; ETK1; HEK; HEK4; TYRO4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209191 representing NM_005233
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTGTCAGCTCTCCATCCTCCTCCTTCTCAGCTGCTCTGTTCTCGACAGCTTCGGGGAACTGATTC
  CGCAGCCTTCCAATGAAGTCAATCTACTGGATTCAAAAACAATTCAAGGGGAGCTGGGCTGGATCTCTTA
  TCCATCACATGGGTGGGAAGAGATCAGTGGTGTGGATGAACATTACACACCCATCAGGACTTACCAGGTG
  TGCAATGTCATGGACCACAGTCAAAACAATTGGCTGAGAACAAACTGGGTCCCCAGGAACTCAGCTCAGA
  AGATTTATGTGGAGCTCAAGTTCACTCTACGAGACTGCAATAGCATTCCATTGGTTTTAGGAACTTGCAA
  GGAGACATTCAACCTGTACTACATGGAGTCTGATGATGATCATGGGGTGAAATTTCGAGAGCATCAGTTT
  ACAAAGATTGACACCATTGCAGCTGATGAAAGTTTCACTCAAATGGATCTTGGGGACCGTATTCTGAAGC
  TCAACACTGAGATTAGAGAAGTAGGTCCTGTCAACAAGAAGGGATTTTATTTGGCATTTCAAGATGTTGG
  TGCTTGTGTTGCCTTGGTGTCTGTGAGAGTATACTTCAAAAAGTGCCCATTTACAGTGAAGAATCTGGCT
  ATGTTTCCAGACACGGTACCCATGGACTCCCAGTCCCTGGTGGAGGTTAGAGGGTCTTGTGTCAACAATT
  CTAAGGAGGAAGATCCTCCAAGGATGTACTGCAGTACAGAAGGCGAATGGCTTGTACCCATTGGCAAGTG
  TTCCTGCAATGCTGGCTATGAAGAAAGAGGTTTTATGTGCCAAGCTTGTCGACCAGGTTTCTACAAGGCA
  TTGGATGGTAATATGAAGTGTGCTAAGTGCCCGCCTCACAGTTCTACTCAGGAAGATGGTTCAATGAACT
  GCAGGTGTGAGAATAATTACTTCCGGGCAGACAAAGACCCTCCATCCATGGCTTGTACCCGACCTCCATC
  TTCACCAAGAAATGTTATCTCTAATATAAACGAGACCTCAGTTATCCTGGACTGGAGTTGGCCCCTGGAC
  ACAGGAGGCCGGAAAGATGTTACCTTCAACATCATATGTAAAAAATGTGGGTGGAATATAAAACAGTGTG
  AGCCATGCAGCCCAAATGTCCGCTTCCTCCCTCGACAGTTTGGACTCACCAACACCACGGTGACAGTGAC
  AGACCTTCTGGCACATACTAACTACACCTTTGAGATTGATGCCGTTAATGGGGTGTCAGAGCTGAGCTCC
  CCACCAAGACAGTTTGCTGCGGTCAGCATCACAACTAATCAGGCTGCTCCATCACCTGTCCTGACGATTA
  AGAAAGATCGGACCTCCAGAAATAGCATCTCTTTGTCCTGGCAAGAACCTGAACATCCTAATGGGATCAT
  ATTGGACTACGAGGTCAAATACTATGAAAAGCAGGAACAAGAAACAAGTTATACCATTCTGAGGGCAAGA
  GGCACAAATGTTACCATCAGTAGCCTCAAGCCTGACACTATATACGTATTCCAAATCCGAGCCCGAACAG
  CCGCTGGATATGGGACGAACAGCCGCAAGTTTGAGTTTGAAACTAGTCCAGACTCTTTCTCCATCTCTGG
  TGAAAGTAGCCAAGTGGTCATGATCGCCATTTCAGCGGCAGTAGCAATTATTCTCCTCACTGTTGTCATC
  TATGTTTTGATTGGGAGGTTCTGTGGCTATAAGTCAAAACATGGGGCAGATGAAAAAAGACTTCATTTTG
  GCAATGGGCATTTAAAACTTCCAGGTCTCAGGACTTATGTTGACCCACATACATATGAAGACCCTACCCA
  AACTGTTCATGAGTTTGCCAAGGAATTGGATGCCACCAACATATCCATTGATAAAGTTGTTGGAGCAGGT
  GAATTTGGAGAGGTGTGCAGTGGTCGCTTAAAACTTCCTTCAAAAAAAGAGATTTCAGTGGCCATTAAGA
  CCCTGAAAGTTGGCTACACAGAAAAGCAGAGGAGAGACTTCCTGGGAGAAGCAAGCATTATGGGACAGTT
  TGACCACCCCAATATCATTCGACTGGAAGGAGTTGTTACCAAAAGTAAGCCAGTTATGATTGTCACAGAA
  TACATGGAGAATGGTTCCTTGGATAGTTTCCTACGTAAACACGATGCCCAGTTTACTGTCATTCAGCTAG
  TGGGGATGCTTCGAGGGATAGCATCTGGCATGAAGTACCTGTCAGACATGGGCTATGTTCACCGAGACCT
  CGCTGCTCGGAACATCTTGATCAACAGTAACTTGGTGTGTAAGGTTTCTGATTTCGGACTTTCGCGTGTC
  CTGGAGGATGACCCAGAAGCTGCTTATACAACAAGAGGAGGGAAGATCCCAATCAGGTGGACATCACCAG
  AAGCTATAGCCTACCGCAAGTTCACGTCAGCCAGCGATGTATGGAGTTATGGGATTGTTCTCTGGGAGGT
  GATGTCTTATGGAGAGAGACCATACTGGGAGATGTCCAATCAGGATGTAATTAAAGCTGTAGATGAGGGC
  TATCGACTGCCACCCCCCATGGACTGCCCAGCTGCCTTGTATCAGCTGATGCTGGACTGCTGGCAGAAAG
  ACAGGAACAACAGACCCAAGTTTGAGCAGATTGTTAGTATTCTGGACAAGCTTATCCGGAATCCCGGCAG
  CCTGAAGATCATCACCAGTGCAGCCGCAAGGCCATCAAACCTTCTTCTGGACCAAAGCAATGTGGATATC
  ACTACCTTCCGCACAACAGGTGACTGGCTTAATGGTGTCTGGACAGCACACTGCAAGGAAATCTTCACGG
  GTGTGGAGTACAGTTCTTGTGACACAATAGCCAAGATTTCCACAGATGACATGAAAAAGGTTGGTGTCAC
  CGTGGTTGGGCCACAGAAGAAGATCATCAGTAGCATTAAAGCTCTAGAAACGCAATCAAAGAATGGCCCA
  GTTCCCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209191 representing NM_005233
  Red=Cloning site Green=Tags(s)
  
  MDCQLSILLLLSCSVLDSFGELIPQPSNEVNLLDSKTIQGELGWISYPSHGWEEISGVDEHYTPIRTYQV
  CNVMDHSQNNWLRTNWVPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNLYYMESDDDHGVKFREHQF
  TKIDTIAADESFTQMDLGDRILKLNTEIREVGPVNKKGFYLAFQDVGACVALVSVRVYFKKCPFTVKNLA
  MFPDTVPMDSQSLVEVRGSCVNNSKEEDPPRMYCSTEGEWLVPIGKCSCNAGYEERGFMCQACRPGFYKA
  LDGNMKCAKCPPHSSTQEDGSMNCRCENNYFRADKDPPSMACTRPPSSPRNVISNINETSVILDWSWPLD
  TGGRKDVTFNIICKKCGWNIKQCEPCSPNVRFLPRQFGLTNTTVTVTDLLAHTNYTFEIDAVNGVSELSS
  PPRQFAAVSITTNQAAPSPVLTIKKDRTSRNSISLSWQEPEHPNGIILDYEVKYYEKQEQETSYTILRAR
  GTNVTISSLKPDTIYVFQIRARTAAGYGTNSRKFEFETSPDSFSISGESSQVVMIAISAAVAIILLTVVI
  YVLIGRFCGYKSKHGADEKRLHFGNGHLKLPGLRTYVDPHTYEDPTQTVHEFAKELDATNISIDKVVGAG
  EFGEVCSGRLKLPSKKEISVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIRLEGVVTKSKPVMIVTE
  YMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRV
  LEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVLWEVMSYGERPYWEMSNQDVIKAVDEG
  YRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSILDKLIRNPGSLKIITSAAARPSNLLLDQSNVDI
  TTFRTTGDWLNGVWTAHCKEIFTGVEYSSCDTIAKISTDDMKKVGVTVVGPQKKIISSIKALETQSKNGP
  VPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005233
• ORF Size: 2949 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005233.6
• RefSeq Size: 5826 bp
• RefSeq ORF: 2952 bp
• Locus ID: 2042
• UniProt ID: P29320
• Cytogenetics: 3p11.1
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Secreted Protein, Transmembrane
• Protein Pathways: Axon guidance
• MW: 110 kDa
• Gene Summary: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]