ST6GALNAC3 (NM_152996) Human Tagged ORF Clone

CAT#: RC209177

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ST6GALNAC3 (Myc-DDK-tagged)-Human ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 3 (ST6GALNAC3), transcript variant 1



  "NM_152996" in other vectors (4)

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USD 420.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name ST6GALNAC3 (NM_152996) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ST6GALNAC3
Synonyms PRO7177; SIAT7C; ST6GALNACIII; STY
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC209177 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTGCATCCTGAAGAGAAAGTCTGTGATTGCTGTGAGCTTCATAGCAGCGTTCCTTTTCCTGCTGG
TTGTGCGTCTTGTAAATGAAGTGAATTTCCCATTGCTACTAAACTGCTTTGGACAACCTGGTACAAAGTG
GATACCATTCTCCTACACATACAGGCGGCCCCTTCGAACTCACTATGGATACATAAATGTGAAGACACAA
GAGCCTTTGCAACTGGACTGTGACCTTTGTGCCATAGTGTCAAACTCAGGTCAGATGGTTGGCCAGAAGG
TGGGAAATGAGATAGATCGATCCTCCTGCATTTGGAGAATGAACAATGCCCCCACCAAAGGTTATGAAGA
AGATGTCGGCCGCATGACCATGATTCGAGTTGTGTCCCATACCAGCGTTCCTCTTTTGCTAAAAAACCCT
GATTATTTTTTCAAGGAAGCGAATACTACTATTTATGTTATTTGGGGACCTTTCCGCAATATGAGGAAAG
ATGGCAATGGCATCGTTTACAACATGTTGAAAAAGACAGTTGGTATCTATCCGAATGCCCAAATATACGT
GACCACAGAGAAGCGCATGAGTTACTGTGATGGAGTTTTTAAGAAGGAAACTGGGAAGGACAGAGTCCAG
TCTGGCTCATATCTCAGCACAGGGTGGTTTACCTTCATTCTGGCCATGGACGCCTGTTATGGCATTCACG
TCTACGGGATGATAAATGACACCTACTGCAAGACAGAAGGGTATAGAAAAGTCCCCTACCATTATTATGA
ACAAGGAAGAGATGAGTGTGATGAATATTTTCTTCATGAACATGCCCCATATGGGGGTCATAGGTTTATC
ACTGAAAAGAAAGTGTTTGCTAAATGGGCCAAGAAGCACAGGATAATATTTACACATCCAAACTGGACAT
TGTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209177 protein sequence
Red=Cloning site Green=Tags(s)

MACILKRKSVIAVSFIAAFLFLLVVRLVNEVNFPLLLNCFGQPGTKWIPFSYTYRRPLRTHYGYINVKTQ
EPLQLDCDLCAIVSNSGQMVGQKVGNEIDRSSCIWRMNNAPTKGYEEDVGRMTMIRVVSHTSVPLLLKNP
DYFFKEANTTIYVIWGPFRNMRKDGNGIVYNMLKKTVGIYPNAQIYVTTEKRMSYCDGVFKKETGKDRVQ
SGSYLSTGWFTFILAMDACYGIHVYGMINDTYCKTEGYRKVPYHYYEQGRDECDEYFLHEHAPYGGHRFI
TEKKVFAKWAKKHRIIFTHPNWTLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_152996
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_152996.1, NM_152996.2, NM_152996.3, NP_694541.1
RefSeq Size 3259 bp
RefSeq ORF 918 bp
Locus ID 256435
Cytogenetics 1p31.1
Domains Glyco_transf_29
Protein Families Transmembrane
Protein Pathways Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
MW 35.4 kDa
Gene Summary ST6GALNAC3 belongs to a family of sialyltransferases that transfer sialic acids from CMP-sialic acid to terminal positions of carbohydrate groups in glycoproteins and glycolipids (Lee et al., 1999 [PubMed 10207017]). [supplied by OMIM, Mar 2008]

Other Versions

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